Karakteristik dan Stabilitas Fisik NLC-Koenzim Q10 dalam Sleeping Mask dengan Minyak Nilam

Pendahuluan: Minyak nilam memiliki efek antioksidan dan peningkat penetrasi. Minyak tersebut berpotensi meningkatkan efektivitas produk sleeping mask dengan Koenzim Q10 (KoQ10) yang dimuat dalam Nanostructured Lipid Carriers (NLC) sebagai kosmetik anti-penuaan. Tujuan: Membandingkan karakteristik dan stabilitas fisik dari NLC-KoQ10 yang dimuat dalam sleeping mask dengan dan tanpa minyak nilam. Metode: Preparasi NLC-KoQ10 menggunakan metode High Shear Homogenization. NLC-KoQ10 dicampur dengan hydrogel dan minyak nilam, untuk F2. Sedangkan untuk F1 tanpa minyak nilam. Setelah itu diamati karakteristik dan stabilitas fisiknya yang meliputi organoleptik, pH, dan viskositas. Uji stabilitas fisik diamati pada suhu ruang selama 90 hari. Hasil: Uji karakteristik fisik menunjukkan bahwa F1 memiliki bau seperti oleum cacao, sedangkan F2 memiliki bau khas minyak nilam dan sedikit bau seperti oleum cacao. F1 memiliki nilai pH 6,036 ± 0,011, sedangkan F2 memiliki nilai pH 6,062 ± 0,020. Tidak ada perbedaan yang signifikan. Namun, F1 dan F2 memiliki nilai viskositas yang berbeda. F1 memiliki nilai viskositas 199,2 ± 0,7 cp, sedangkan F2 memiliki nilai viskositas 175,6 ± 7,9 cp. Uji stabilitas fisik menunjukkan bahwa F1 dan F2 memiliki skala nilai pH berkisar 6,055 - 6,336 dan viskositas 175,6 - 239,7 cp. Nilai viskositas F1 mengalami peningkatan setelah hari ke-60, sedangkan F2 pada hari ke-90. Kesimpulan: Berdasarkan uji karakteristik dan stabilitas fisik dapat disimpulkan bahwa F1 dan F2 memiliki bau dan viskositas yang berbeda, dan F2 lebih stabil daripada F1.

Licochalcone A-loaded solid lipid nanoparticles improve antischistosomal activity in vitro and in vivo

Aim: To isolate licochalcone A (LicoA) from licorice, prepare LicoA-loaded solid lipid nanoparticles (L-SLNs) and evaluate the L-SLNs in vitro and in vivo against Schistosoma mansoni. Materials & methods: LicoA was obtained by chromatographic fractionation and encapsulated in SLNs by a modified high shear homogenization method. Results: L-SLNs showed high encapsulation efficiency, with satisfactory particle size, polydispersity index and Zeta potential. Transmission electron microscopy revealed that L-SLNs were rounded and homogenously distributed. Toxicity studies revealed that SLNs decreased the hemolytic and cytotoxic properties of LicoA. Treatment with L-SLNs showed in vivo efficacy against S. mansoni. Conclusion: L-SLNs are efficient in reducing worm burden and SLNs may be a promising delivery system for LicoA to treat S. mansoni infections.

Olanzapine Loaded Nanostructured Lipid Carriers via High Shear Homogenization and Ultrasonication

The aim of this study was to understand the effect of high shear homogenization (HSH) and ultrasonication (US) on the physicochemical properties of blank and olanzapine loaded nanostructured lipid carriers (NLCs) along with their drug loading potential and drug release profiles from formulated particles. NLCs were prepared with different ratios of Compritol and Miglyol as the solid and liquid lipids, respectively, under changing HSH and US times between 0 to 15 minutes. The surfactants (Poloxamer 188 (P188) and tween 80) and the drug content was kept constant in all formulations. The prepared NLCs were evaluated for particle size, polydispersity index, zeta potential, drug crystallinity and chemical interactions between lipids and OLZ. The in-vitro drug release was performed using dialysis tube method in phosphate buffer solution (PBS) at pH 7.4. The formulated NLCs were negatively charged, spherically shaped and monodisperse, with particle sizes ranging from 112 to 191 nm. There was a significant influence of US time on the preparation of NLCs in comparison to HSH, where a significant reduction in the mean particle diameter was seen after 5 min of sonication. An increase of Miglyol content in NLCs led to an increase in particle size. In general, application of US led to decrease in particle size after HSH but an increase in particle diameter of low Miglyol containing preparation was also observed with longer sonication time. OLZ was successfully encapsulated in the NLCs and a total release of 89% was achieved in 24 hours in PBS at pH 7.4.

Types of Solid Lipids on Physical Stability of Resveratrol-Loaded Nanostructured Lipid Carriers

Resveratrol (Res) loaded nanostructured lipid carriers (NLCs) were prepared by high shear homogenization and ultrasonication technique. Caprylic/capric glyceride (IM) was used as a liquid lipid, while glyceryl monostearate (GMS), beeswax (BW), palmitic acid (PA) and myristic acid (MA) at various concentrations (5, 10, 15, and 20 %w/w) were solid lipids. Polyoxyethylene 20 sorbitan monooleate or polysorbate 80 (Tween® 80) was use as a surfactant. The results showed that all concentration of PA and MA can be prepared the smaller particle NLC than that using GMS and BW according to the small molecule of MA and PA. The zeta potential of Res loaded NLCs were negative charge (-24.30 to -37.80 mV), which could be considered as a stable system. The stability studies revealed that NLCs with MA and PA showed a - high stability in particle size and zeta potential after storage at 40 ± 2°C /75 %RH for 3 months. These results suggested the MA and PA were suitable solid lipids for the NLCs preparation which have potential to be a nanoparticulate carrier for Res delivery.