scholarly journals Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach

2008 ◽  
Vol 73A (5) ◽  
pp. 400-410 ◽  
Author(s):  
Bridget E. McLaughlin ◽  
Nicole Baumgarth ◽  
Martin Bigos ◽  
Mario Roederer ◽  
Stephen C. De Rosa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
T Cell Subsets ◽  
Color Flow ◽  
Cytokine Responses ◽  
Panel Design

scholarly journals Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part II: Panel performance across different instrument platforms

2008 ◽  
Vol 73A (5) ◽  
pp. 411-420 ◽  
Author(s):  
Bridget E. McLaughlin ◽  
Nicole Baumgarth ◽  
Martin Bigos ◽  
Mario Roederer ◽  
Stephen C. De Rosa ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
T Cell Subsets ◽  
Color Flow ◽  
Cytokine Responses

Heterogeneous calcium flux in peripheral T cell subsets revealed by five-color flow cytometry using log-ratio circuitry

Cytometry ◽  
1995 ◽  
Vol 21 (2) ◽  
pp. 187-196 ◽  
Author(s):  
M. Roederer ◽  
M. Bigos ◽  
T. Nozaki ◽  
R. T. Stovel ◽  
D. R. Parks ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
T Cell Subsets ◽  
Calcium Flux ◽  
Color Flow ◽  
Log Ratio

scholarly journals Characterization of CD4-Positive Lymphocytes in the Antiviral Response of Olive Flounder (Paralichthys oliveceus) to Nervous Necrosis Virus

2020 ◽  
Vol 21 (11) ◽  
pp. 4180
Author(s):  
Jae Wook Jung ◽  
Jin Hong Chun ◽  
Jung Seok Lee ◽  
Si Won Kim ◽  
Ae Rin Lee ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Immune Response ◽  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Viral Infection ◽  
T Cell Subsets ◽  
Olive Flounder ◽  
Flow Cytometry Analysis ◽  
Color Flow

The presence of CD4 T lymphocytes has been described for several teleost species, while many of the main T cell subsets have not been characterized at a cellular level, because of a lack of suitable tools for their identification, e.g., monoclonal antibodies (mAbs) against cell markers. We previously described the tissue distribution and immune response related to CD3ε and CD4-1 T cells in olive flounder (Paralichthys oliveceus) in response to a viral infection. In the present study, we successfully produce an mAb against CD4-2 T lymphocytes from olive flounder and confirmed its specificity using immuno-blotting, immunofluorescence staining, flow cytometry analysis and reverse transcription polymerase chain reaction (RT-PCR). Using these mAbs, we were able to demonstrate that the CD3ε T cell populations contain both types of CD4+ cells, with the majority of the CD4 T cell subpopulations being CD4-1+/CD4-2+ cells, determined using two-color flow cytometry analysis. We also examined the functional activity of the CD4-1 and CD4-2 cells in vivo in response to a viral infection, with the numbers of both types of CD4 T cells increasing significantly during the virus infection. Collectively, these findings suggest that the CD4 T lymphocytes in olive flounder are equivalent to the helper T cells in mammals in terms of their properties and function, and it is the CD4-2 T lymphocytes rather than the CD4-1 T cells that play an important role in the Th1 immune response against viral infections in olive flounder.

scholarly journals Development of a Flow Cytometry Assay to Predict Immune Checkpoint Blockade-Related Complications

2021 ◽  
Vol 12 ◽  
Author(s):  
Hannah-Lou Schilling ◽  
Gunther Glehr ◽  
Michael Kapinsky ◽  
Norbert Ahrens ◽  
Paloma Riquelme ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
T Cell ◽  
Immune Checkpoint ◽  
Predictive Value ◽  
Memory T Cells ◽  
T Cell Subsets ◽  
Effector Memory ◽  
Color Flow ◽  
Skin Reactions

Treatment of advanced melanoma with combined immune checkpoint inhibitor (ICI) therapy is complicated in up to 50% of cases by immune-related adverse events (irAE) that commonly include hepatitis, colitis and skin reactions. We previously reported that pre-therapy expansion of cytomegalovirus (CMV)-reactive CD4+ effector memory T cells (TEM) predicts ICI-related hepatitis in a subset of patients with Stage IV melanoma given αPD-1 and αCTLA-4. Here, we develop and validate a 10-color flow cytometry panel for reliably quantifying CD4+ TEM cells and other biomarkers of irAE risk in peripheral blood samples. Compared to previous methods, our new panel performs equally well in measuring CD4+ TEM cells (agreement = 98%) and is superior in resolving CD4+ CD197+ CD45RA- central memory T cells (TCM) from CD4+ CD197+ CD45RA+ naive T cells (Tnaive). It also enables us to precisely quantify CD14+ monocytes (CV = 6.6%). Our new “monocyte and T cell” (MoT) assay predicts immune-related hepatitis with a positive predictive value (PPV) of 83% and negative predictive value (NPV) of 80%. Our essential improvements open the possibility of sharing our predictive methods with other clinical centers. Furthermore, condensing measurements of monocyte and memory T cell subsets into a single assay simplifies our workflows and facilitates computational analyses.

Characterization of T-cell subsets and T-cell receptor subgroups in pigtailed macaques using two- and three-color flow cytometry

1991 ◽  
Vol 11 (4) ◽  
pp. 193-204 ◽  
Author(s):  
Inger Axberg ◽  
Michael J. Gale ◽  
Bijan Afar ◽  
Edward A. Clark
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
Color Flow

Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes

Cytometry ◽  
2002 ◽  
Vol 50 (2) ◽  
pp. 92-101 ◽  
Author(s):  
Jan W. Gratama ◽  
Jaco Kraan ◽  
Mike Keeney ◽  
Viv Granger ◽  
David Barnett
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Cell Subset ◽  
Color Flow ◽  
T Cell Subset

scholarly journals Modulation of CD4+ and CD8+ T Cell Function and Cytokine Responses in Strongyloides stercoralis Infection by Interleukin-27 (IL-27) and IL-37

2017 ◽  
Vol 85 (11) ◽  
Author(s):  
Rajamanickam Anuradha ◽  
Saravanan Munisankar ◽  
Yukthi Bhootra ◽  
Chandrakumar Dolla ◽  
Paul Kumaran ◽  
...  
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
Strongyloides Stercoralis ◽  
T Cell Response ◽  
T Cell Subsets ◽  
Content Type ◽  
Antigen Stimulation ◽  
Parasite Antigen ◽  
Cytokine Responses ◽  
Interleukin 27

ABSTRACT Strongyloides stercoralis infection is associated with diminished antigen-specific Th1- and Th17-associated responses and enhanced Th2-associated responses. Interleukin-27 (IL-27) and IL-37 are two known anti-inflammatory cytokines that are highly expressed in S. stercoralis infection. We therefore wanted to examine the role of IL-27 and IL-37 in regulating CD4+ and CD8+ T cell responses in S. stercoralis infection. To this end, we examined the frequency of Th1/Tc1, Th2/Tc2, Th9/Tc9, Th17/Tc17, and Th22/Tc22 cells in 15 S. stercoralis-infected individuals and 10 uninfected individuals stimulated with parasite antigen following IL-27 or IL-37 neutralization. We also examined the production of prototypical type 1, type 2, type 9, type 17, and type 22 cytokines in the whole-blood supernatants. Our data reveal that IL-27 or IL-37 neutralization resulted in significantly enhanced frequencies of Th1/Tc1, Th2/Tc2, Th17/Tc17, Th9, and Th22 cells with parasite antigen stimulation. There was no induction of any T cell response in uninfected individuals following parasite antigen stimulation and IL-27 or IL-37 neutralization. Moreover, we also observed increased production of gamma interferon (IFN-γ), IL-5, IL-9, IL-17, and IL-22 and decreased production of IL-10 following IL-27 and IL-37 neutralization and parasite antigen stimulation in whole-blood cultures. Thus, we demonstrate that IL-27 and IL-37 limit the induction of particular T cell subsets along with cytokine responses in S. stercoralis infections, which suggest the importance of IL-27 and IL-37 in immune modulation in a chronic helminth infection.

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