Flow cytometric quantitation of natural killer cells and T lymphocytes expressing T-cell receptors alpha/beta and gamma/delta is not helpful in distinguishing benign from malignant body cavity effusions

Abstract We show further differences between two clinically related entities, T- cell acute lymphoblastic leukemia (T-ALL) and lymphoblastic lymphoma (T- LL), by using several monoclonal antibodies (MoAbs) reacting with either constant or variable regions of T-cell receptors (TcR) alpha beta and gamma delta or with various CD molecules. We analyzed a panel of 15 T-ALL and 15 T-LL selected for their cell surface expression of the CD3 molecules. The results indicated that TcR gamma delta is more frequently used than TcR alpha beta in T-ALL (10 of the 15 patients tested). This is in contrast to the results obtained with T-LL where the vast majority expressed TcR alpha beta (13 of the 15 patients). These findings suggest that the leukemic cells could have a different origin in these two diseases. In addition, analysis of TcR variable regions expressed by the leukemic blasts showed that, in most cases, they had rearranged functional V delta 1 gene segments (8 of 11 patients), whereas in a unique case V delta 2 gene segment was used. Together, these results and those indicating that T-ALL cells coexpress the CD1a, b, and c molecules strengthen the possibility that although these leukemic cells express the CD3-TcR complex at their cell surface, their normal counterparts are not found in peripheral blood.

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Control of self-reactive cytotoxic T lymphocytes expressing γδ T cell receptors by natural killer inhibitory receptors

European Journal of Immunology ◽

10.1002/eji.1830271111 ◽

1997 ◽

Vol 27 (11) ◽

pp. 2812-2821 ◽

Cited By ~ 112

Author(s):

Franck Halary ◽

Marie-Alix Peyrat ◽

Eric Champagne ◽

Miguel Lopez-Botet ◽

Alessandro Moretta ◽

...

Keyword(s):

T Cell ◽

T Lymphocytes ◽

Natural Killer ◽

Cytotoxic T Lymphocytes ◽

T Cell Receptors ◽

Γδ T Cell ◽

Inhibitory Receptors ◽

Cell Receptors ◽

Killer Inhibitory Receptors

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Small nucleolar RNAs are concomitantly down-regulated during murine gamma delta T-cell development.

10.31219/osf.io/b3sa5 ◽

2019 ◽

Author(s):

Shahan Mamoor

Keyword(s):

T Cells ◽

T Cell ◽

T Cell Receptors ◽

T Cell Development ◽

Cell Development ◽

Gamma Delta T Cells ◽

Gamma Delta ◽

Cell Receptors ◽

Alpha Beta ◽

Gamma Delta T Cell

Gamma delta T-cells are a lymphocyte subset that display gamma delta T-cell receptors rather than the alpha beta T-cell receptors that alpha beta T cells like CD4 helper and CD8 cytotoxic T-cells display, and whose function straddles the intersection of innate and adaptive immune cells (1). To understand the transcriptional behavior of gamma delta T-cells during mammalian development, we performed global differential gene expression of datasets encompassing transcriptome data from embryonic and adult gamma delta T-cells from mice (2). These analyses revealed a species of non-coding RNA termed small nucleolar RNA, or snoRNA were among the most differentially expressed genes when comparing embryonic and adult gamma delta T-cells. Moreover, these snoRNA were uniformly down-regulated over the course of gamma delta T-cell development. These data demonstrate unprecedented developmental repression of snoRNA in lymphocytes and suggest that stage-specific repression of snoRNAs may serve some vital developmental purpose in the function of gamma delta T-cells.

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Study of the CD3-associated T-cell receptors reveals further differences between T-cell acute lymphoblastic lymphoma and leukemia

Blood ◽

10.1182/blood.v75.4.931.bloodjournal754931 ◽

1990 ◽

Vol 75 (4) ◽

pp. 931-934

Author(s):

C Gouttefangeas ◽

A Bensussan ◽

L Boumsell

Keyword(s):

T Cell ◽

Cell Surface ◽

T Cell Receptors ◽

Lymphoblastic Lymphoma ◽

Leukemic Cells ◽

Cell Surface Expression ◽

Gamma Delta ◽

Variable Regions ◽

Cell Receptors ◽

Alpha Beta

We show further differences between two clinically related entities, T- cell acute lymphoblastic leukemia (T-ALL) and lymphoblastic lymphoma (T- LL), by using several monoclonal antibodies (MoAbs) reacting with either constant or variable regions of T-cell receptors (TcR) alpha beta and gamma delta or with various CD molecules. We analyzed a panel of 15 T-ALL and 15 T-LL selected for their cell surface expression of the CD3 molecules. The results indicated that TcR gamma delta is more frequently used than TcR alpha beta in T-ALL (10 of the 15 patients tested). This is in contrast to the results obtained with T-LL where the vast majority expressed TcR alpha beta (13 of the 15 patients). These findings suggest that the leukemic cells could have a different origin in these two diseases. In addition, analysis of TcR variable regions expressed by the leukemic blasts showed that, in most cases, they had rearranged functional V delta 1 gene segments (8 of 11 patients), whereas in a unique case V delta 2 gene segment was used. Together, these results and those indicating that T-ALL cells coexpress the CD1a, b, and c molecules strengthen the possibility that although these leukemic cells express the CD3-TcR complex at their cell surface, their normal counterparts are not found in peripheral blood.

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