Cytology of high-grade squamous intraepithelial lesion in Japanese-Brazilian women with HIV infection with polymerase chain reaction-assisted human papilloma virus detection

2002 ◽  
Vol 26 (4) ◽  
pp. 268-271
Author(s):  
Tadao K. Kobayashi ◽  
Masami Ueda ◽  
Toshihiro Nishino ◽  
Suzuko Moritani ◽  
Yoshiro Hanada ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hiv Infection ◽  
Human Papilloma Virus ◽  
Virus Detection ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Intraepithelial Lesion ◽  
Polymerase Chain

Human papilloma virus detection in liquid cytology, in situ hybridization and polymerase chain reaction

2005 ◽  
Vol 446 (2) ◽  
pp. 202-203 ◽  
Author(s):  
F. Alameda ◽  
L. Pijuan ◽  
L. Ferrer ◽  
M. L. Mari�oso ◽  
M. Muset ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Human Papilloma Virus ◽  
Virus Detection ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain

p16 and Ki-67 Immunostaining in Atypical Immature Squamous Metaplasia of the Uterine Cervix: Correlation With Human Papillomavirus Detection

2007 ◽  
Vol 131 (9) ◽  
pp. 1343-1349 ◽  
Author(s):  
Lori Iaconis ◽  
Elizabeth Hyjek ◽  
Lora H. Ellenson ◽  
Edyta C. Pirog
Keyword(s):  
Polymerase Chain Reaction ◽  
Squamous Metaplasia ◽  
Human Papillomaviruses ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Chain Reaction ◽  
Ki 67 ◽  
Intraepithelial Lesion ◽  
Polymerase Chain ◽  
Cervical Mucosa

Abstract Context.—Atypical immature squamous metaplasia (AIM) of the cervix is a loosely defined entity characterized by immature metaplastic cells with mild cytologic atypia. Objective.—To examine whether a combination of immunostaining for p16 and Ki-67 could be used to stratify AIM cases into 3 categories: benign, cases with nondiagnostic atypia, and high-grade squamous intraepithelial lesion (HSIL). Design.—The study consisted of 37 cases of AIM, 23 cases of benign cervical mucosa (NEG), and 36 cases of HSIL. All cases were tested for high-risk human papillomaviruses using SPF 10 polymerase chain reaction and immunostained for p16 and Ki-67. Results.—All cases of HSIL were positive for both p16 and Ki-67. All but 2 benign control cases were negative for both p16 and Ki-67. Seven cases of AIM (19%) displayed a pattern of immunostaining identical to HSIL, and these most likely represent a spectrum of HSIL. A total of 54% of cases of AIM were negative for both p16 and Ki-67, consistent with benign reactive atypia. Two AIM cases (5%) were negative for p16 and positive for Ki-67 in the area adjacent to an ulcer, representing regeneration. Finally, 22% of AIM cases were positive for p16 and negative for Ki-67; such cases may represent a precursor of HSIL or, alternatively, a regressing HSIL. Conclusion.—The combination of immunostaining for p16 and Ki-67 is helpful in limiting of the number of cases with nondiagnostic atypia of the cervix.

scholarly journals Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia

2014 ◽  
Vol 66 (1) ◽  
pp. 429-436
Author(s):  
Biserka Vukomanovic-Djurdjevic ◽  
Gordana Basta-Jovanovic ◽  
N. Baletic ◽  
Milica Berisavac ◽  
D. Nenadic ◽  
...  
Keyword(s):  
High Risk ◽  
Human Papilloma Virus ◽  
Morphometric Analysis ◽  
Cervical Dysplasia ◽  
Epithelial Dysplasia ◽  
Control Group ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Papilloma Virus ◽  
Intraepithelial Lesion

Genomic integration of high-risk human papilloma virus in the nucleus of cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of this study was to determine the relevance of correlation between epithelial survivin expression and the degree of human papilloma virus (HPV)-induced cervical epithelial dysplasia, and to establish the significance of morphometric analysis of the nuclear area in the assessment of the degree of cervical dysplasia. This retrospective study included 99 women with primary, previously untreated lesions, and colposcopic findings indicating dysplasia, in whom a cytological test by Papanicolaou method was interpreted according to the Bethesda criteria as lowgrade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and atypical squamous cells of undetermined significance (ASCUS). We performed human papilloma virus (HPV) typing by PCR for evidence of viruse types 16, 18, 31, 33. After biopsy of the cervical mucosa, we performed hematoxylin-eosin (H-E) and Periodic Acid Schiff (PAS) staining, and immunohistochemical and morphometric analysis of tissue samples. The control group consisted of 12 women without dysplasia and without a verified infection of cervical high-risk HPV. A high statistical correlation between the degree of dysplasia and expression of survivin was found in patients with different types of cervical dysplasia (p = 0.003). We observed a high statistical difference between the area of nuclei at different degrees of cervical dysplasias (p = 0.000). The high-grade cervical dysplasia had a more than 2-fold higher level of ranking in comparison to low-grade dysplasia, and a more than 10-fold higher ranking than the control group without cervical dysplasia.

Uptake of exogenous human papilloma virus L1 DNA by oocytes and detection by the polymerase chain reaction

1992 ◽  
Vol 9 (6) ◽  
pp. 531-533 ◽  
Author(s):  
Philip J. Chan ◽  
Brian C. Su ◽  
Donald R. Tredway ◽  
Majid Seraj ◽  
Ibrahim M. Seraj ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain

scholarly journals Presencia de lesiones preinvasoras e invasoras de cérvix, relación con el virus papiloma humano y factores epidemiológicos en Mérida, Venezuela

2018 ◽  
Vol 2 (2) ◽  
pp. 92
Author(s):  
Luis Eduardo Téllez Gil ◽  
Elvia María Michelli Viña ◽  
Diana Estela Callejas Monsalve ◽  
Mike Contreras Colmenares ◽  
María Eugenia Cavazza Porro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Viral Infection ◽  
Smoking Habit ◽  
Multiple Infections ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Collection Device ◽  
Dna Collection ◽  
Polymerase Chain

  Las lesiones de cérvix se han asociado a infección por Virus Papiloma Humano (VPH). 300 mujeres mayores de quince años que acudieron al Hospital Universitario de Los Andes (HULA), fueron estudiadas para identificar lesiones, detectar y tipificar VPH, y determinar factores asociados. Se realizó citología, colposcopia, cepillados cervicales utilizando (DNA collection device Digene®) y biopsias en los casos pertinentes. Se aisló el ADN mediante (QIAamp DNA Mini Kit QIAGEN®), siendo cuantificado y almacenado a -20 ºC. Se detectó VPH por Reacción en Cadena de la Polimerasa (PCR) de regiones L1 y E6/E7. La genotipificación por PCR anidada múltiple E6/E7, C. trachomatis se detectó por PCR. El VPH se detectó en 35 % (105) muestras, 88,46 % (92/105) fueron positivas para al menos uno de los genotipos evaluados. VPHAR se encontraron en 97,82 %, (90/92), VPH18 en 82 % (74/90), VPH16 en 44 % (40/90). 56,52 % (52/92) correspondieron a infecciones múltiples, VPH18/16 (20/52) fue la más frecuente. C. trachomatis se detectó en 9 % (27/300) pacientes. La citología mostró cambios sugestivos de infección en solo 16,35 % de las pacientes VPH positivas. 17/18 biopsias sugirieron infección viral y fueron positivas para VPH AR por biología molecular (94,44 %). La colposcopia sugirió infección viral en 46,15 %. El 66,34 % de pacientes fueron menores de 35 años. Se encontró relación estadísticamente no significativa entre infección por VPH, número de parejas sexuales, coinfección con C. trachomatis y hábito tabáquico. Estos resultados muestran elevada frecuencia de infección por VPH AR, asociada a factores epidemiológicos, cuyo diagnóstico certero y tratamiento oportuno son claves en la prevención de su transmisión y del desarrollo de lesiones en cérvix.   Palabras clave: Cáncer cervical, virus papiloma humano, reacción en cadena de la polimerasa.   Abstract   Cervical lesions have been associated with infection by Human Papilloma Virus (HPV). Three hundred women older than 15 years old who attended at the Hospital Universidad de Los Andes (HULA), were studied to identify lesions, detect and typify HPV, and determine associated factors. Cytology, colposcopy, cervical brushing using (DNA collection device Digene®) and biopsies were performed in the pertinent cases. DNA was isolated by (QIAamp DNA Mini Kit QIAGEN®), being quantified and stored at -20 ° C. HPV was detected by Polymerase Chain Reaction (PCR) of regions L1 and E6 / E7. The genotyping by multiple nested PCR E6 / E7, C. trachomatis was detected by PCR. HPV was detected in 35% (105) samples, 88.46% (92/105) were positive for at least one of the genotypes evaluated. VPHAR were found in 97.82% (90/92), HPV18 in 82% (74/90), HPV16 in 44% (40/90). 56.52% (52/92) corresponded to multiple infections, HPV18 / 16 (20/52) was the most frequent. C. trachomatis was detected in 9% (27/300) patients. The cytology showed changes suggestive of infection in only 16.35% of the HPV positive patients. 17/18 biopsies suggested viral infection and were positive for ARV HPV by molecular biology (94.44%). Colposcopy suggested viral infection in 46.15%. 66.34% of patients were under 35 years old. A statistically non-significant relationship was found between HPV infection, number of sexual partners, coinfection with C. trachomatis and smoking habit. These results show high frequency of infection by HPV AR, associated with epidemiological factors, whose accurate diagnosis and timely treatment are key in the prevention of its transmission and the development of lesions in the cervix.   Keywords: Cervical cancer, human papilloma virus, polymerase chain reaction.

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