Advanced glycation end products-induced apoptosis attenuated by PPARδ activation and epigallocatechin gallate through NF-κB pathway in human embryonic kidney cells and human mesangial cells

2010 ◽  
Vol 26 (5) ◽  
pp. 406-416 ◽  
Author(s):  
Yao-Jen Liang ◽  
Jhin-Hao Jian ◽  
Yuan-Chun Liu ◽  
Shiow-Jen Juang ◽  
Kou-Gi Shyu ◽  
...  
Keyword(s):  
Mesangial Cells ◽  
Epigallocatechin Gallate ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Advanced Glycation ◽  
Human Mesangial Cells ◽  
Human Embryonic Kidney Cells ◽  
Glycation End Products ◽  
End Products ◽  
Induced Apoptosis

scholarly journals DJC Suppresses Advanced Glycation End Products-Induced JAK-STAT Signaling and ROS in Mesangial Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Min Sun ◽  
Yan Li ◽  
Wenjie Bu ◽  
Jindong Zhao ◽  
Jianliang Zhu ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Advanced Glycation End Products ◽  
Oxidase Activity ◽  
Mesangial Cells ◽  
Dependent Manner ◽  
Advanced Glycation ◽  
Nadph Oxidase Activity ◽  
Glycation End Products ◽  
End Products ◽  
Anti Inflammatory

The antidiabetic properties and anti-inflammatory effects of Danzhi Jiangtang Capsules (DJC) have been demonstrated in clinical and laboratory experiments. In this study, we explored whether DJC can ameliorate advanced glycation end products- (AGEs-) mediated cell injury and the precise mechanisms of DJC in treating diabetic nephropathy (DN). Western blot analysis was employed to assess the expressions of iNOS, COX2, and SOCS and the phosphorylation of JAK2, STAT1, and STAT3 in glomerular mesangial cells (GMCs) after treatment with DJC. TNF-α, IL-6, and MCP-1 were determined using double-antibody sandwich ELISA. ROS and NADPH oxidase activity were measured by DCFH-DA assay and lucigenin-enhanced chemiluminescence, respectively. DJC significantly reversed the AGEs-induced expression of COX2 and iNOS. Moreover, DJC inhibited the AGEs-induced JAK2-STAT1/STAT3 activation, resulting in the inhibition of inflammatory cytokines such as IL-6, MCP-1, and TNF-αin a concentration-dependent manner. The ability of DJC to suppress STAT activation was also verified by the observation that DJC significantly increased the SOCS3 protein level. DJC reversed the AGEs-induced accumulation of ROS and NADPH oxidase activity, thus confirming that DJC possesses antioxidant activity. The results suggest that the anti-inflammatory effects of DJC in GMCs may be due to its ability to suppress the JAK2-STAT1/STAT3 cascades and reduce ROS production.

Effects of Advanced Glycation End Products on the mRNA Expression of Urotensin II and G Protein-Coupled Receptor in Rat Mesangial Cells

2013 ◽  
Vol 781-784 ◽  
pp. 746-751
Author(s):  
Yan Zhao ◽  
Feng Wu Lin ◽  
Cai Li ◽  
Xiang Jun Li ◽  
Yan Shi ◽  
...  
Keyword(s):  
Mesangial Cells ◽  
Urotensin Ii ◽  
Rt Pcr ◽  
Advanced Glycation ◽  
Control Groups ◽  
Glycation End Products ◽  
End Products ◽  
The Impact ◽  
G Protein Coupled

To investigate the impact of AGEs with different concentrations and different times on the expression of UⅡ and GPR14 mRNA in MC. Methods In vitro rat mesangial cells, by adding different concentrations of AGEs(final concentration0、25、50、100和200 mg/L)，37°C incubated for 24 h,AGE100 mg/L groups were cultured 0、2、8、16 and 24 h separately，established the control groups respectively, collecting MC,extracted total cellular RNA for RT-PCR reaction and detected the expression of UⅡ and GPR14 mRNA in MC. Results The expression of UⅡ and GPR14 mRNA in MC were increased with the change of AGEs concentrations from low to high.Compared with AGEs 0 mg / L group, the expression of UⅡ and GPR14 mRNA in AGEs50, 100, and 200 mg / L groups were significantly higher (P<0.05); In AGEs100mg / L groups, the expression of UⅡ and GPR14 mRNA in MC were increased with the time extended, compared with AGEs 0h group, the expression of UⅡ and GPR14 mRNA in AGEs8, 16, and 24h groups were significantly higher (P<0.05); There were no change in control groups（P＞0.05）. Conclusion AGEs can significantly increase the expression of UⅡ and GPR14 mRNA in MC.

scholarly journals Transcriptomic and Proteomic Analysis Reveals Mechanisms of Patulin-Induced Cell Toxicity in Human Embryonic Kidney Cells

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 681
Author(s):  
Nianfa Han ◽  
Ruilin Luo ◽  
Jiayu Liu ◽  
Tianmin Guo ◽  
Jiayu Feng ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Digital Gene Expression ◽  
Hek293 Cells ◽  
Kidney Cells ◽  
Oxygen Species ◽  
Human Embryonic Kidney ◽  
Absolute Quantitation ◽  
Human Embryonic Kidney Cells ◽  
Isobaric Tags ◽  
Induced Apoptosis

Patulin (PAT) is a natural mycotoxin that commonly contaminates fruits and fruit-based products. Previous work indicated that PAT-induced apoptosis in which reactive oxygen species (ROS) are involved in human embryonic kidney (HEK293) cells. To uncover novel aspects of the possible mechanism of PAT nephrotoxicity, the transcriptome and proteome profiles were investigated using the digital gene expression (DGE) and isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approaches. A total of 127 genes and 85 proteins were found to express differentially in response to 5 μM PAT for 10 h in HEK293 cells. The most dramatic changes of expression were noticed with genes or proteins related to apoptosis, oxidative phosphorylation ribosome and cell cycle. Especially, the activation of caspase 3, UQCR11, active transport form and endocytosis appeared to be crucial in PAT kidney cytotoxicity. PAT also seemed to be associated with cancer and neuropathic disease as pathways associated with carcinogenesis, Alzheimer’s disease and Parkinson’s disease were induced. Overall, this study served to uncover overall insights associated with signaling pathway that modulated the PAT toxicity mechanism.

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