scholarly journals Embryo ecology: Developmental synchrony and asynchrony in the embryonic development of wild annual fish populations

2021 ◽  
Author(s):  
Matej Polačik ◽  
Milan Vrtílek ◽  
Martin Reichard ◽  
Jakub Žák ◽  
Radim Blažek ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Fish Populations ◽  
Annual Fish

Rapid growth and large body size in annual fish populations are compromised by density‐dependent regulation

2019 ◽  
Vol 95 (2) ◽  
pp. 673-678 ◽  
Author(s):  
Milan Vrtílek ◽  
Jakub Žák ◽  
Matej Polačik ◽  
Radim Blažek ◽  
Martin Reichard
Keyword(s):  
Body Size ◽  
Rapid Growth ◽  
Large Body ◽  
Fish Populations ◽  
Large Body Size ◽  
Annual Fish ◽  
Density Dependent ◽  
Density Dependent Regulation

scholarly journals The influence of temperature on the embryonic development of the annual fish Cynopoecilus melanotaenia (Cyprinodontiformes, Rivulidae)

2002 ◽  
Vol 62 (4b) ◽  
pp. 743-747 ◽  
Author(s):  
A. ARENZON ◽  
C. A. LEMOS ◽  
M. B. C. BOHRER
Keyword(s):  
Embryonic Development ◽  
Developmental Stages ◽  
Test Organism ◽  
Developmental Rate ◽  
Developmental Time ◽  
Laboratory Culture ◽  
Toxicity Tests ◽  
Hatching Rate ◽  
Annual Fish ◽  
Significant Difference

The present study aims to provide data about the time required for Cynopoecilus melanotaenia kept at different temperatures to complete embryonic development. This information can be valuable for optimizing laboratory culture and facilitating future use of this species as a test organism in toxicity tests. Temperature effects on hatching rate are presented as well as information related to embryonic development stages. Eggs were observed daily, from start to finish of embryonic development. Thirteen developmental stages were described. Eggs were kept at two constant temperatures (20°C and 25°C) and at a variable ambient temperature (16-25°C - mean = 21°C, sd = 1.95), to determine developmental rate (velocity) at each temperature. A shorter incubation period was necessary to complete development at 25° ± 1°C. However, all embryos kept at this temperature hatched with morphological defects, which prevented their survival. No significant difference in developmental time period (p = 0.05) was observed at the 20°C and 16°-25°C (mean = 21°C, sd = 1.95) temperatures.

scholarly journals Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology

EvoDevo ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Luca Dolfi ◽  
Roberto Ripa ◽  
Adam Antebi ◽  
Dario Riccardo Valenzano ◽  
Alessandro Cellerino
Keyword(s):  
Cell Cycle ◽  
Embryonic Development ◽  
Mitotic Activity ◽  
Developmental Trajectories ◽  
Dispersed Phase ◽  
Quiescent State ◽  
Entry And Exit ◽  
Annual Fish ◽  
Cell Cycle Dynamics ◽  
Lateral Axis

Abstract Background Annual killifishes are adapted to surviving and reproducing over alternating dry and wet seasons. During the dry season, all adults die and desiccation-resistant embryos remain encased in dry mud for months or years in a state of diapause where their development is halted in anticipation of the months that have to elapse before their habitats are flooded again. Embryonic development of annual killifishes deviates from canonical teleost development. Epiblast cells disperse during epiboly, and a “dispersed phase” precedes gastrulation. In addition, annual fish have the ability to enter diapause and block embryonic development at the dispersed phase (diapause I), mid-somitogenesis (diapause II) and the final phase of development (diapause III). Developmental transitions associated with diapause entry and exit can be linked with cell cycle events. Here we set to image this transition in living embryos. Results To visibly explore cell cycle dynamics during killifish development in depth, we created a stable transgenic line in Nothobranchius furzeri that expresses two fluorescent reporters, one for the G1 phase and one for the S/G2 phases of the cell cycle, respectively (Fluorescent Ubiquitination-based Cell Cycle Indicator, FUCCI). Using this tool, we observed that, during epiboly, epiblast cells progressively become quiescent and exit the cell cycle. All embryos transit through a phase where dispersed cells migrate, without showing any mitotic activity, possibly blocked in the G1 phase (diapause I). Thereafter, exit from diapause I is synchronous and cells enter directly into the S phase without transiting through G1. The developmental trajectories of embryos entering diapause and of those that continue to develop are different. In particular, embryos entering diapause have reduced growth along the medio-lateral axis. Finally, exit from diapause II is synchronous for all cells and is characterized by a burst of mitotic activity and growth along the medio-lateral axis such that, by the end of this phase, the morphology of the embryos is identical to that of direct-developing embryos. Conclusions Our study reveals surprising levels of coordination of cellular dynamics during diapause and provides a reference framework for further developmental analyses of this remarkable developmental quiescent state.

The diet of otters (Lutra lutra L.) in Danish freshwater habitats: comparisons of prey fish populations

1999 ◽  
Vol 248 (1) ◽  
pp. 1-13 ◽  
Author(s):  
H.-M. Taastrøm ◽  
L. Jacobsen
Keyword(s):  
Fish Populations ◽  
Lutra Lutra ◽  
Prey Fish ◽  
Freshwater Habitats

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

ADHD therapy with methylphenidate in mothers – a danger for embryonic development?

2014 ◽  
Vol 47 (06) ◽  
Author(s):  
N Bergemann ◽  
K Boyle ◽  
WE Paulus
Keyword(s):  
Embryonic Development

A Laboratory Study of Fish Populations

1947 ◽  
Vol 74 (1) ◽  
pp. 350-359 ◽  
Author(s):  
Hurst H. Shoemaker
Keyword(s):  
Laboratory Study ◽  
Fish Populations
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