Surface markers of cloned human T cells with helper or suppressor activity on pokeweed mitogen-driven B cell differentiation

Abstract Interleukin-11 (IL-11) is a recently described stromal-derived cytokine that supports the growth of an IL-6-dependent murine plasmacytoma line in the presence of antibody to IL-6 and appears to act in a manner similar to IL-6 on hematopoietic stem cells. Because IL-6 is known to promote differentiation of normal human B cells, the role of IL-11 on B- cell differentiation in vitro was characterized. IL-11 does not result in significantly increased DNA synthesis or Ig secretion by purified B cells alone or B cells cultured with Staphylococcus Cowan I, a T-cell- independent B-cell mitogen. In contrast, purified B cells cultured in the presence of pokeweed mitogen (PWM), irradiated T cells, and monocytes show increased DNA synthesis at day 3 and increased IgG and IgM secretion at day 7 of culture; addition of IL-11 further augments Ig secretion without change in DNA synthesis, an effect that can only be partially blocked by monoclonal antibody to IL-6. Similar experiments confirmed that increased IgG secretion was demonstrable when either IL-11 or IL-6 was added to B cells + CD4+/45RA- T cells + monocytes + PWM; in contrast, Ig secretion was low and equivalent when CD4+/45RA+ T cells were cultured with B cells+monocytes+PWM with or without IL-6 or IL-11. Neither IL-6 nor IL-11 could significantly increase phytohemagglutinin (PHA)-induced DNA synthesis by CD4+/45RA- or CD4+/45RA+ T cells. Although PWM or IL-11 induced IL-6 mRNA expression in both CD4+/45RA- T cells and monocytes, in neither cell did IL-11 increase IL-6 mRNA expression over that noted to PWM alone. These observations support the view that IL-11 promotes differentiation of human B lymphocytes only in the presence of accessory T cells and monocytes and that a minor component of this effect may be through stimulation of IL-6 production by CD4+/45RA- T cells and monocytes.

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Humoral helper activity for B-cell differentiation released from non- Hodgkin's lymphoma cells having both SRBC and complement receptors in the pokeweed mitogen system

Blood ◽

10.1182/blood.v57.6.1074.1074 ◽

1981 ◽

Vol 57 (6) ◽

pp. 1074-1080

Author(s):

N Moriya ◽

T Miyawaki ◽

Y Ueno ◽

S Koizumi ◽

N Taniguchi

Keyword(s):

T Cells ◽

Cell Differentiation ◽

B Cells ◽

B Cell ◽

Plasma Cells ◽

Lymphoid Cells ◽

Pokeweed Mitogen ◽

Complement Receptors ◽

B Cell Differentiation ◽

Cell Leukemia

Abstract The majority of lymphoid cells from a patient with non-Hodgkin's lymphoma with leukemic transformation were demonstrated to carry receptors for both sheep erythrocytes and complements by the combined rosette assay using neuraminidase-treated sheep erythrocytes and complement-coated zymosan beads. Most of them were considered morphologically lymphoblasts and were positive for acid phosphatase staining. Terminal deoxynucleotidyl transferase activity was not detected in these cells. Lymphoid cells from this patient did not respond to the stimulation with phytohemagglutinin-P, concanavalin-A, and pokeweed mitogen (PWM). When these cells were cultured with PWM for 7 days, no plasma cell was generated. Although only a few plasma cells were generated in the PWM-stimulated culture of normal purified B cells alone, the addition of the patient's cells to purified normal B cells resulted in a markedly enhanced generation of plasma cells in response to PWM, as was the case with normal T cells. But leukemic cells either from a patient with T-cell leukemia not having complement receptors or from a patient with null-cell leukemia showed no enhancing ability in B- cell differentiation. In addition, the culture supernates of the patient's cells obtained after 24-hr PWM stimulation had an ability to promote B-cell differentiation comparable in activity to those from the PWM-stimulated normal T cells.

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Induction of Inhibitory Activity for B Cell Differentiation in Human CD8 T Cells with Pokeweed Mitogen, Dimaprit, and cAMP Upregulating Agents: Countersuppressive Effect of Platelet Factor 4

Cellular Immunology ◽

10.1006/cimm.1996.0234 ◽

1996 ◽

Vol 172 (2) ◽

pp. 205-216 ◽

Cited By ~ 10

Author(s):

Giovanna M. Crisi ◽

Irene R. Katz ◽

Marjorie B. Zucker ◽

G.Jeanette Thorbecke

Keyword(s):

T Cells ◽

Cell Differentiation ◽

B Cell ◽

Cd8 T Cells ◽

Inhibitory Activity ◽

Platelet Factor 4 ◽

Pokeweed Mitogen ◽

B Cell Differentiation ◽

Platelet Factor

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Humoral helper activity for B-cell differentiation released from non- Hodgkin's lymphoma cells having both SRBC and complement receptors in the pokeweed mitogen system

Blood ◽

10.1182/blood.v57.6.1074.bloodjournal5761074 ◽

1981 ◽

Vol 57 (6) ◽

pp. 1074-1080 ◽

Cited By ~ 6

Author(s):

N Moriya ◽

T Miyawaki ◽

Y Ueno ◽

S Koizumi ◽

N Taniguchi

Keyword(s):

T Cells ◽

Cell Differentiation ◽

B Cells ◽

B Cell ◽

Plasma Cells ◽

Lymphoid Cells ◽

Pokeweed Mitogen ◽

Complement Receptors ◽

B Cell Differentiation ◽

Cell Leukemia

The majority of lymphoid cells from a patient with non-Hodgkin's lymphoma with leukemic transformation were demonstrated to carry receptors for both sheep erythrocytes and complements by the combined rosette assay using neuraminidase-treated sheep erythrocytes and complement-coated zymosan beads. Most of them were considered morphologically lymphoblasts and were positive for acid phosphatase staining. Terminal deoxynucleotidyl transferase activity was not detected in these cells. Lymphoid cells from this patient did not respond to the stimulation with phytohemagglutinin-P, concanavalin-A, and pokeweed mitogen (PWM). When these cells were cultured with PWM for 7 days, no plasma cell was generated. Although only a few plasma cells were generated in the PWM-stimulated culture of normal purified B cells alone, the addition of the patient's cells to purified normal B cells resulted in a markedly enhanced generation of plasma cells in response to PWM, as was the case with normal T cells. But leukemic cells either from a patient with T-cell leukemia not having complement receptors or from a patient with null-cell leukemia showed no enhancing ability in B- cell differentiation. In addition, the culture supernates of the patient's cells obtained after 24-hr PWM stimulation had an ability to promote B-cell differentiation comparable in activity to those from the PWM-stimulated normal T cells.

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Interleukin-11 promotes accessory cell-dependent B-cell differentiation in humans

Blood ◽

10.1182/blood.v80.11.2797.bloodjournal80112797 ◽

1992 ◽

Vol 80 (11) ◽

pp. 2797-2804 ◽

Cited By ~ 42

Author(s):

KC Anderson ◽

C Morimoto ◽

SR Paul ◽

D Chauhan ◽

D Williams ◽

...

Keyword(s):

T Cells ◽

Cell Differentiation ◽

B Cells ◽

Dna Synthesis ◽

B Cell ◽

Mrna Expression ◽

Pokeweed Mitogen ◽

B Cell Differentiation ◽

Interleukin 11 ◽

Cells Cultured

Interleukin-11 (IL-11) is a recently described stromal-derived cytokine that supports the growth of an IL-6-dependent murine plasmacytoma line in the presence of antibody to IL-6 and appears to act in a manner similar to IL-6 on hematopoietic stem cells. Because IL-6 is known to promote differentiation of normal human B cells, the role of IL-11 on B- cell differentiation in vitro was characterized. IL-11 does not result in significantly increased DNA synthesis or Ig secretion by purified B cells alone or B cells cultured with Staphylococcus Cowan I, a T-cell- independent B-cell mitogen. In contrast, purified B cells cultured in the presence of pokeweed mitogen (PWM), irradiated T cells, and monocytes show increased DNA synthesis at day 3 and increased IgG and IgM secretion at day 7 of culture; addition of IL-11 further augments Ig secretion without change in DNA synthesis, an effect that can only be partially blocked by monoclonal antibody to IL-6. Similar experiments confirmed that increased IgG secretion was demonstrable when either IL-11 or IL-6 was added to B cells + CD4+/45RA- T cells + monocytes + PWM; in contrast, Ig secretion was low and equivalent when CD4+/45RA+ T cells were cultured with B cells+monocytes+PWM with or without IL-6 or IL-11. Neither IL-6 nor IL-11 could significantly increase phytohemagglutinin (PHA)-induced DNA synthesis by CD4+/45RA- or CD4+/45RA+ T cells. Although PWM or IL-11 induced IL-6 mRNA expression in both CD4+/45RA- T cells and monocytes, in neither cell did IL-11 increase IL-6 mRNA expression over that noted to PWM alone. These observations support the view that IL-11 promotes differentiation of human B lymphocytes only in the presence of accessory T cells and monocytes and that a minor component of this effect may be through stimulation of IL-6 production by CD4+/45RA- T cells and monocytes.

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