Improved sulfoalkylbetaine-based organic-silica hybrid monolith for high efficient hydrophilic interaction liquid chromatography of polar compounds

2013 ◽  
Vol 34 (13) ◽  
pp. 1877-1885 ◽  
Author(s):  
Yingzhuang Chen ◽  
Keyi Wang ◽  
Yixuan Liu ◽  
Huihui Yang ◽  
Shouzhuo Yao ◽  
...  
Separations ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 6 ◽  
Author(s):  
Irene Panderi ◽  
Eugenia Taxiarchi ◽  
Constantinos Pistos ◽  
Eleni Kalogria ◽  
Ariadni Vonaparti

Bisphosphonates are used to treat various skeletal disorders, as they modulate bone metabolism by inhibition of the osteoclast-mediated bone resorption. These compounds are both polar and ionic, and therefore, by using reversed phase liquid chromatography are eluted rapidly. Hydrophilic interaction liquid chromatography (HILIC) is an advantageous technique for the separation and analysis of polar molecules. As the elution order in HILIC is reversed to reversed phase liquid chromatography, a reasonable retention and selectivity for polar compounds is expected. In this work the retention mechanism of three bisphosponates, namely risedronate, tiludronate and zoledronate, was investigated under zwitterionic HILIC conditions. The key factors influencing the retention of the analytes on a zwitterionic ZIC®-pHILIC column (150.0 × 2.1 mm i.d., 200 Å, 3.5 μm) have been systematically investigated. It was found that apart from partition, electrostatic repulsions play an important role in the retention of bisphosphonates. Peak tailing of risedronate and zoledronate was improved by the addition of sodium pyrophosphate in the mobile phase. A zwitterionic hydrophilic interaction liquid chromatography-photodiode array (HILIC-PDA) method was further optimized and fully validated to quantitate risedronate in commercial film-coated tablets. The calibration curves for risedronate showed good linearity (r > 0.9991) within the calibration range tested. The intra- and inter-day coefficient of variation (CV) values was less than 0.6%, while the relative percentage error (%Er) was less than −2.3%. Accelerated stability studies of risedronate conducted under several degradation conditions including hydrolysis, oxidation and heat demonstrated the selectivity of the procedure. A short-run analysis of not more than 6 min allowed the analysis of large samples per day. The applicability of the method for the quantitation of risedronate was demonstrated via the analysis of commercial tablets containing this compound.


2020 ◽  
Vol 16 ◽  
Author(s):  
Cem Erkmen ◽  
Weldejeworgıs Hndeya Gebrehiwot ◽  
Bengi Uslu

Background: Significant advances have been occurred in analytical research since the 1970s by liquid chromatography (LC) as the separation method. Reverse phase liquid chromatography (RPLC) method, using hydrophobic stationary phases and polar mobile phases is the most commonly used chromatographic method. However, it is difficult to analyze some polar compounds with this method. Another separation method, normal phase liquid chromatography (NPLC), which involves polar stationary phases with organic eluents. NPLC presents low-efficiency separations and asymmetric chromatographic peak shapes when analyzing polar compounds. Hydrophilic interaction liquid chromatography (HILIC) is an interesting and promising alternative method for the analysis of polar compounds. HILIC is be defined a separation method which combines stationary phases used in the NPLC method and mobile phases used in RPLC method. HILIC can be successfully applied to all types of liquid chromatographic separations such as pharmaceutical compounds, small molecules, metabolites, drugs of abuse, carbohydrates, toxins, oligosaccharides, peptides, amino acids and proteins. Objective: This paper provides a general overview of the recent application of HILIC in the pharmaceutical research in the different sample matrices such as pharmaceutical dosage form, plasma, serum, environmental samples, animal origin samples, plant origin samples, etc. Also, this review focuses on the most recent and selected papers in the drug research from 2009 to submission date in 2020 dealing with the analysis of different components using HILIC. Results and Conclusion: The literature survey showed that HILIC applications are increasing every year in pharmaceutical research. It was found that HILIC allows simultaneous analysis of many compounds using different detectors.


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