scholarly journals The dynamics of T cells during persistent Staphylococcus aureus infection: from antigen‐reactivity to in vivo anergy

2011 ◽  
Vol 3 (11) ◽  
pp. 652-666 ◽  
Author(s):  
Christina Ziegler ◽  
Oliver Goldmann ◽  
Elias Hobeika ◽  
Robert Geffers ◽  
Georg Peters ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
T Cells ◽  
Aureus Infection ◽  
Antigen Reactivity

Prevention of Panton-Valentine Toxin-Mediated Leucocyte Destruction Depending on the Ultra-Diluted Staphylococcinum Potency Gradient

2020 ◽  
Vol 33 (02) ◽  
pp. 099-103
Author(s):  
Pritam Goswami ◽  
Sayak Ghosh ◽  
Sk Swaif Ali ◽  
Anamika Basu ◽  
Joydeep Khanra ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ecological Niche ◽  
Staphylococcal Infection ◽  
Buffy Coat ◽  
Preventive Action ◽  
Phagocytic Cells ◽  
Mortality And Morbidity ◽  
Aureus Infection ◽  
The Mean

Abstract Background Panton-Valentine toxin (PVT) is an important pathological marker of staphylococcal infection mediated by functional as well as morphological damage of the phagocytic cells. Human body being an ecological niche for the bacteria shows higher affinity toward staphylococcal infection. A steady escalation in mortality and morbidity associated with antibiotic resistance in gram-positive infections is an emerging threat all over the globe; thus, it is important to find out an alternative strategy that can diminish the virulence and pathogenicity of the bacteria. Staphylococcin is a colicin-type chemical secreted by Staphylococcus aureus helps to prevent growth of organisms other than its progenitor. In this study, we evaluated the efficacy of homoeopathic medicine Staphylococcinum against staphylococcal PVT at different potencies (6CH, 12CH, 30CH and 200CH). Materials and Methods Different potencies of Staphylococcinum were administered in a leucocyte buffy coat preparation infected with staphylococcal suspension (0.5 McFarland's standard) along with control with alcohol. They were kept in incubator for 2 hours and then centrifuged at 1200 rpm for 5 minutes. Smears prepared on slides with centrifuged deposits stained by Preston and Morrell's modified Gram's method of staining and evaluated under the microscope. Results It was observed that there was extensive destruction of leukocytes in control and 6CH potency, while the degree of destruction decreased markedly from 12CH to 200CH. At 200CH potency, leukocytes were almost normal, which clearly indicate the preventive action of Staphylococcinum against PVT. The mean percentages of intact leucocytes were 0.73, 0.93, 10.00, 27.67 and 65.00 in control, 6CH, 12CH, 30CH and 200CH potencies, respectively. Conclusion The finding may help in the use of this medicine in moribund patients in cases of disseminated S. aureus infection as there is no known side effect of the medicine. However, in vivo study is necessary before such use in those cases.

In vivo photothermal inhibition of methicillin-resistant Staphylococcus aureus infection by in situ templated formulation of pathogen-targeting phototheranostics

Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7651-7659 ◽  
Author(s):  
Xujuan Guo ◽  
Bing Cao ◽  
Congyu Wang ◽  
Siyu Lu ◽  
Xianglong Hu
Keyword(s):  
Staphylococcus Aureus ◽  
Magnetic Resonance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Photoacoustic Imaging ◽  
Methicillin Resistant ◽  
Aureus Infection ◽  
Mrsa Infection ◽  
Efficient Elimination

Herein, pathogen-targeting phototheranostic nanoparticles, Van-OA@PPy, are in situ developed for efficient elimination of MRSA infection, which is reflected by dual-modality magnetic resonance and photoacoustic imaging.

scholarly journals IL-22 derived from γδ T cells restricts Staphylococcus aureus infection of mechanically injured skin

2016 ◽  
Vol 138 (4) ◽  
pp. 1098-1107.e3 ◽  
Author(s):  
Nidhi Malhotra ◽  
Juhan Yoon ◽  
Juan Manuel Leyva-Castillo ◽  
Claire Galand ◽  
Nathan Archer ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
T Cells ◽  
Γδ T Cells ◽  
Aureus Infection ◽  
Injured Skin

DEGRADATION OF PENICILLIN G AND THREE SEMISYNTHETIC PENICILLINS BY BACILLUS CEREUS AND STAPHYLOCOCCUS AUREUS PENICILLINASE

1966 ◽  
Vol 12 (1) ◽  
pp. 35-42 ◽  
Author(s):  
J. A. Yurchenco ◽  
M. W. Hopper ◽  
G. H. Warren
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Enzymatic Degradation ◽  
Penicillin G ◽  
Aureus Infection ◽  
Penicillanic Acid ◽  
Potassium Penicillin ◽  
And Staphylococcus Aureus

An in vivo procedure is described for determining the relative sensitivities of potassium penicillin G and three semisynthetic penicillins to degradation by Bacillus cereus and Staphylococcus aureus penicillinases. The inactivating concentrations (IC50) of the penicillinases necessary to reduce the protective activity of each of the penicillins against an S. aureus infection in mice from PD95 to a PD50 level was determined. Conventional in vitro studies were carried out for purposes of comparison. After interaction with B. cereus penicillinase, Wy-3206 [6-(2-methoxy-1-naphthamido) penicillanic acid] had the greatest residual therapeutic activity, followed in order by nafcillin [6-(2-ethoxy-1-naphthamido)penicillanic acid], methicillin [sodium 6-(2, 6-dimethoxybenzamido)penicillinate monohydrate], and potassium penicillin G. Penicillin G proved to be the most sensitive to enzymatic degradation by S. aureus penicillinase, whereas nafcillin and methicillin were resistant to the highest concentration employed. These findings were, in general, supported by the in vitro results.

Liu Shen Wan inhibits influenza virus-induced secondary Staphylococcus aureus infection in vivo and in vitro

2021 ◽  
pp. 114066
Author(s):  
Jin Zhao ◽  
Xiaodong Huang ◽  
Yutao Wang ◽  
Qinhai Ma ◽  
Jian Song ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Influenza Virus ◽  
Aureus Infection

scholarly journals Apoptosis Induced by Staphylococcus aureus in Epithelial Cells Utilizes a Mechanism Involving Caspases 8 and 3

2000 ◽  
Vol 68 (5) ◽  
pp. 2998-3001 ◽  
Author(s):  
Carla A. Wesson ◽  
James Deringer ◽  
Linda E. Liou ◽  
Kenneth W. Bayles ◽  
Gregory A. Bohach ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
T Cells ◽  
Tumor Necrosis ◽  
Cellular Responses ◽  
Mammary Epithelial ◽  
Necrosis Factor Alpha ◽  
Proteolytic Cascade ◽  
Factor Alpha ◽  
Necrosis Factor

ABSTRACT In this study, we demonstrate that the mechanism by whichStaphylococcus aureus induces apoptosis in bovine mammary epithelial (MAC-T) cells involves caspases 8 and 3, two key components of a proteolytic cascade leading to apoptosis. In addition, internalized S. aureus induces expression of the inflammatory cytokines tumor necrosis factor alpha and interleukin-1β by MAC-T cells. These data suggest that the internalization of S. aureus could induce specific cellular responses in vivo that may ultimately impact the course of infection.

scholarly journals Rapid and Broad Immune Efficacy of a Recombinant Five-Antigen Vaccine against Staphylococcus aureus Infection in Animal Models

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 134 ◽  
Author(s):  
Hao Zeng ◽  
Feng Yang ◽  
Qiang Feng ◽  
Jinyong Zhang ◽  
Jiang Gu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Severe Disease ◽  
Lethal Dose ◽  
Protein A ◽  
Surface Proteins ◽  
Staphylococcal Protein A ◽  
Humoral Immune ◽  
Aureus Infection

Staphylococcus aureus (S. aureus) is a leading cause of both healthcare-and community-associated infections globally, which result in severe disease and readily developing antibiotic resistance. Developing an efficacious vaccine against S. aureus is urgently required. In the present study, we selected five conserved antigens, including the secreted factors α-hemolysin (Hla), staphylococcal enterotoxin B (SEB) and the three surface proteins staphylococcal protein A (SpA), iron surface determinant B N2 domain (IsdB-N2) and manganese transport protein C (MntC). They were all well-characterized virulence factor of S. aureus and developed a recombinant five-antigen S. aureus vaccine (rFSAV), rFSAV provided consistent protection in S. aureus lethal sepsis and pneumonia mouse models, and it showed broad immune protection when challenged with a panel of epidemiologically relevant S. aureus strains. Meanwhile, rFSAV immunized mice were able to induce comprehensive cellular and humoral immune responses to reduce bacterial loads, inflammatory cytokine expression, inflammatory cell infiltration and decrease pathology after challenge with a sub-lethal dose of S. aureus. Moreover, the importance of specific antibodies in protection was demonstrated by antibody function tests in vitro and in vivo. Altogether, our data demonstrate that rFSAV is a potentially promising vaccine candidate for defensing against S. aureus infection.

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