Potential application of Thermomyces lanuginosus lipase (TLL) immobilized on nonporous polystyrene particles

2018 ◽  
Vol 38 (2) ◽  
pp. 608-613 ◽  
Author(s):  
Adriana Dantas ◽  
Alexsandra Valério ◽  
Jorge Luiz Ninow ◽  
J. Vladimir de Oliveira ◽  
Débora de Oliveira
Keyword(s):  
Potential Application ◽  
Thermomyces Lanuginosus ◽  
Thermomyces Lanuginosus Lipase

Promoting protein self-association in non-glycosylated Thermomyces lanuginosus lipase based on crystal lattice contacts

2015 ◽  
Vol 1854 (12) ◽  
pp. 1914-1921 ◽  
Author(s):  
Jens Kvist Madsen ◽  
Thomas Rebsdorf Sørensen ◽  
Jørn Døvling Kaspersen ◽  
Maria Berggård Silow ◽  
Jesper Vind ◽  
...  
Keyword(s):  
Crystal Lattice ◽  
Thermomyces Lanuginosus ◽  
Thermomyces Lanuginosus Lipase ◽  
Self Association

scholarly journals Production of Structured Phosphatidylcholine with High Content of Myristic Acid by Lipase-Catalyzed Acidolysis and Interesterification

Catalysts ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 281 ◽  
Author(s):  
Anna Chojnacka ◽  
Witold Gładkowski
Keyword(s):  
Myristic Acid ◽  
Rhizomucor Miehei ◽  
Egg Yolk ◽  
Thermomyces Lanuginosus ◽  
Molar Ratio ◽  
Acyl Donor ◽  
Thermomyces Lanuginosus Lipase ◽  
Screening Experiments ◽  
Miehei Lipase ◽  
Acyl Donors

Synthesis of structured phosphatidylcholine (PC) enriched with myristic acid (MA) was conducted by acidolysis and interesterification reactions using immobilized lipases as catalysts and two acyl donors: trimyristin (TMA) isolated from ground nutmeg, and myristic acid obtained by saponification of TMA. Screening experiments indicated that the most effective biocatalyst for interesterification was Rhizomucor miehei lipase (RML), whereas for acidolysis, the most active were Thermomyces lanuginosus lipase (TLL) and RML. The effect of the molar ratio of substrates (egg-yolk PC/acyl donor), enzyme loading, and different solvent on the incorporation of MA into PC and on PC recovery was studied. The maximal incorporation of MA (44 wt%) was achieved after 48 h of RML-catalyzed interesterification in hexane using substrates molar ratio (PC/trimyristin) 1/5 and 30% enzyme load. Comparable results were obtained in toluene with 1/3 substrates molar ratio. Interesterification of PC with trimyristin resulted in significantly higher MA incorporation than acidolysis with myristic acid, particularly in the reactions catalyzed by RML.

scholarly journals Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis

2020 ◽  
Vol 24 ◽  
pp. 101197
Author(s):  
Jing Li ◽  
Jiandong Zhang ◽  
Shuguang Shen ◽  
Bing Zhang ◽  
William W. Yu
Keyword(s):  
Thermomyces Lanuginosus ◽  
Thermomyces Lanuginosus Lipase ◽  
Biodiesel Synthesis

Competition of Thermomyces lanuginosus lipase with its hydrolysis products at the oil–water interface

2017 ◽  
Vol 149 ◽  
pp. 280-287 ◽  
Author(s):  
Marco Muth ◽  
Stefanie Rothkötter ◽  
Steven Paprosch ◽  
Reiner P. Schmid ◽  
Klaus Schnitzlein
Keyword(s):  
Thermomyces Lanuginosus ◽  
Water Interface ◽  
Thermomyces Lanuginosus Lipase ◽  
Hydrolysis Products ◽  
Oil Water
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