scholarly journals Influence of different SSF conditions on ethanol production from corn stover at high solids loadings

2015 ◽  
Vol 3 (5) ◽  
pp. 481-489 ◽  
Author(s):  
Arne Gladis ◽  
Pia‐Maria Bondesson ◽  
Mats Galbe ◽  
Guido Zacchi
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
High Solids

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

The effect of high solids loading in ethanol production integrated with a pulp mill

2016 ◽  
Vol 111 ◽  
pp. 387-402 ◽  
Author(s):  
Elin Svensson ◽  
Valeria Lundberg ◽  
Mikael Jansson ◽  
Charilaos Xiros ◽  
Thore Berntsson
Keyword(s):  
Ethanol Production ◽  
Pulp Mill ◽  
High Solids ◽  
Solids Loading ◽  
High Solids Loading

scholarly journals Toward Biochemical Conversion of Lignocellulose On-Farm: Pretreatment and Hydrolysis of Corn Stover in Situ

2017 ◽  
Vol 60 (4) ◽  
pp. 1025-1033
Author(s):  
Alicia A. Modenbach ◽  
Sue E. Nokes ◽  
Michael D. Montross ◽  
Barbara L. Knutson
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Conversion Efficiency ◽  
High Solids ◽  
Cellulose Conversion ◽  
Biochemical Conversion ◽  
High Solids Loading ◽  
Combined Pretreatment ◽  
Pretreatment Conditions ◽  
On Farm

Abstract. High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment and hydrolysis. Increasing the enzyme dosage improved cellulose conversion from 9.6% to 36.8% when high-solids loadings were used in both unit operations; however, increasing NaOH loading and pretreatment time did not increase the conversion efficiency. The enzyme-to-substrate ratio had a larger impact on cellulose conversion than the NaOH pretreatment conditions studied, resulting in recommendations for an on-farm bioconversion system. Keywords: Corn stover, Enzymatic hydrolysis, Enzyme loading, High solids, Low solids, Sodium hydroxide.

Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8662-8676
Author(s):  
Maria Mushtaq ◽  
Muhammad Javaid Asad ◽  
Muhammad Zeeshan Hyder ◽  
Syed Muhammad Saqlan Naqvi ◽  
Saad Imran Malik ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Corn Stover ◽  
Rice Straw ◽  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Second Generation Bioethanol ◽  
Simultaneous Saccharification

Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes were used in this experiment. Various parameters, such as acidic and alkali pretreatment, enzymatic hydrolysis by cellulases, and effect of proteases inhibitors on ethanol production, were examined. Fermentation was completed by the yeasts Saccharomyces cerevisiae and Clostridium thermocellum separately, and their ethanol production were compared and maximum ethanol yield was obtained with wheat straw i.e.,11.3 g/L by S. cerevisiae and 8.5 g/L by C. thermocellum. Results indicated that a higher quantity of sugar was obtained from wheat straw (19.6 ± 1.6 g/L) followed by rice straw (17.6 ± 0.6 g/L) and corn stover (16.1 ± 0.9 g/L) compared to the other evaluated biomass samples. A higher yield of ethanol (11.3 g/L) was observed when a glucose concentration of 21.7 g/L was used, for which yeast fermentation efficiency was 92%. Results also revealed the increased in ethanol production (93%) by using celluases in combination with recombinant Serine protease inhibitors from C. thermocellum. It is expected that the use of recombinant serpins with cellulases will play a major role in the biofuel production by using agricultural biomass. This will also help in the economics of the biofuel.

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