scholarly journals Time-lapse waveform analysis for elastic and anelastic structural changes of transducer-transducer active seismic experiments' data during triaxial deformation of granitic rock

2020 ◽  
Author(s):  
Ssu-Ting Lai ◽  
Nobuaki FUJI ◽  
Ikuo Katayama ◽  
Luis Fabian Bonilla ◽  
Yann Capdeville
Keyword(s):  
Structural Changes ◽  
Granitic Rock ◽  
Time Lapse ◽  
Waveform Analysis ◽  
Triaxial Deformation

scholarly journals An instrumented sample holder for time-lapse microtomography measurements of snow under advective airflow

2014 ◽  
Vol 3 (2) ◽  
pp. 179-185 ◽  
Author(s):  
P. P. Ebner ◽  
S. A. Grimm ◽  
M. Schneebeli ◽  
A. Steinfeld
Keyword(s):  
Structural Changes ◽  
Time Lapse ◽  
Temperature Gradients ◽  
Sample Holder ◽  
Experimental Characterization ◽  
Computational Fluid Dynamics Simulations ◽  
Dynamics Simulations ◽  
Snow Samples

Abstract. An instrumented sample holder was developed for time-lapse microtomography of snow samples to enable in situ nondestructive spatial and temporal measurements under controlled advective airflows, temperature gradients, and air humidities. The design was aided by computational fluid dynamics simulations to evaluate the airflow uniformity across the snow sample. Morphological and mass transport properties were evaluated during a 4-day test run. This instrument allows the experimental characterization of metamorphism of snow undergoing structural changes with time.

scholarly journals Dysregulation in EGF/ERBB signaling pathway may explain abnormalities in cellular migration in bipolar disorder in patient-derived neural precursors

2021 ◽  
Author(s):  
Salil.K. Sukumaran ◽  
Pradip Paul ◽  
Vishweshwa Guttal ◽  
Alekhya Vemula ◽  
Harsimar Bhatt ◽  
...  
Keyword(s):  
Bipolar Disorder ◽  
Signaling Pathway ◽  
Structural Changes ◽  
Time Lapse ◽  
Cellular Migration ◽  
Control Line ◽  
Post Mortem ◽  
Post Mortem Brain ◽  
Erbb Signaling ◽  
Erbb Signaling Pathway

ABSTRACTCellular migration is a ubiquitous feature of development that brings brain cells into appropriate spatial relationships. Cortical thinning has been reported in post-mortem brain samples of patients with bipolar disorder (BD). It could be that defective cellular migration during brain development is one of the contributing mechanisms in BD pathogenesis, leading to abnormalities reported at post-mortem, and during brain imaging. To probe the role of cellular migration in BD, we conducted time-lapse analysis of migration of neural precursor cells (NPCs) previously generated in our laboratory. Two NPC lines (B1 and B2) and one control line (C1) were used for the cell migration experiments. Time-lapse images were recorded every 15 min, for 15 hours, and analysed for speed and direction of cellular migration. Abnormalities in cellular migration was a common feature observed in both patient-derived NPCs. Consequently, we investigated underlying mechanistic irregularities in B1 and B2 lines that may contribute to the observed phenotype. To this end, transcriptional changes were compared between the patient-derived cells with the control line. Additionally, we examined exonic variations in genes related to cellular migration or motility. Our analysis showed downregulation of multiple genes that are all part of the EGF/ERBB signaling pathway. Collective dysregulation may be producing the defective cellular phenotype. These cellular migration abnormalities may be linked to structural changes in the brain reported in bipolar disorder.

scholarly journals Tight junction stabilization prevents HepaRG cell death in drug-induced intrahepatic cholestasis

Biology Open ◽  
2021 ◽  
Author(s):  
Rie Sonoi ◽  
Yoshihisa Hagihara
Keyword(s):  
Cell Death ◽  
Structural Changes ◽  
Sodium Taurocholate ◽  
Time Lapse ◽  
Bile Canaliculi ◽  
Dynamic Changes ◽  
Drug Induced ◽  
Heparg Cell ◽  
Kinase Pathway ◽  
Cells Cultured

Entacapone (ENT), a catechol-O-methyltransferase inhibitor, causes liver injury by inducing bile canaliculi (BC) dilation through inhibition of the myosin light kinase pathway. Loss of tight junctions (TJs) induces hepatocyte depolarization, which causes bile secretory failure, leading to liver damage. To understand the influence of TJ structural changes as a consequence of BC dynamics, we compared the datasets of time-lapse and immunofluorescence images for TJ protein ZO-1 in hepatocytes cultured with ENT, forskolin (FOR), ENT/FOR, and those cultured without any drugs. Retrospective analysis revealed that the drastic change in BC behaviors caused TJ disruption and apoptosis in cells cultured with ENT. Exposure to FOR or sodium taurocholate facilitated TJ formation in the cells cultured with ENT and suppressed BC dynamic changes, leading to the inhibition of TJ disruption and apoptosis. Our findings clarify that hepatocyte TJ stabilization protects against cell death induced by BC disruption.

scholarly journals Computer assisted Doppler waveform analysis and ultrasound derived turbulence intensity ratios can predict early hyperplasia development in newly created vascular access fistula: Pilot study, methodology and analysis

2021 ◽  
Vol 10 ◽  
pp. 204800402110001
Author(s):  
Matthew Bartlett ◽  
Vanessa Diaz-Zuccarini ◽  
Janice Tsui
Keyword(s):  
Turbulence Intensity ◽  
Structural Changes ◽  
Neointimal Hyperplasia ◽  
Flow Patterns ◽  
Primary Outcome Measure ◽  
Waveform Analysis ◽  
Secondary Outcome ◽  
Computer Assisted ◽  
Post Surgery ◽  
Intensity Ratios

Objectives Following surgical creation of arterio-venous fistulae (AVF), the desired outward remodeling is often accompanied by the development of neointimal hyperplasia (NIH), which can stymie maturation and may lead to thrombosis and access failure. The aim of this study was to investigate the feasibility of using a non-invasive test, to detect and quantify the turbulent flow patterns believed to be associated with NIH development. Design This was a prospective, observational study. Ultrasound derived turbulence intensity ratios (USTIR) were calculated from spectral Doppler waveforms, recorded from newly formed AVF, and were compared with haemodynamic and structural changes observed during the initial maturation period. Setting Measurements were obtained by accredited Clinical Vascular Scientists, at the Royal Free Hospital, London. Participants Patients with newly created AVF were invited to participate in the study. A total of 30 patients were initially recruited with 19 participants completing the 10 week study protocol. Outcome measures The primary outcome measure was the development of NIH resulting in a haemodynamically significant lesion. The secondary outcome was successful maturation of the AVF at 10 weeks. Results Elevated USTIR in the efferent vein 2 weeks post surgery corresponded to the development of NIH formation (P = 0.02). A cut off of 6.39% predicted NIH development with a sensitivity of 87.5% and a specificity of 80%. Conclusion Analysis of Doppler waveforms can successfully identify deleterious flow patterns and predict inward luminal remodelling in maturing AVF. We propose a longitudinal follow up study to assess the viability of this technique as a surveillance tool.

Time lapse microscopy observation of cellular structural changes and image analysis of drug treated cancer cells to characterize the cellular heterogeneity

2014 ◽  
Vol 30 (6) ◽  
pp. 724-734 ◽  
Author(s):  
Periasamy S. Vaiyapuri ◽  
Alshatwi A. Ali ◽  
Akbarsha A. Mohammad ◽  
Jeyalakshmi Kandhavelu ◽  
Meenakshisundaram Kandhavelu
Keyword(s):  
Image Analysis ◽  
Cancer Cells ◽  
Structural Changes ◽  
Time Lapse ◽  
Cellular Heterogeneity ◽  
Microscopy Observation ◽  
Time Lapse Microscopy

scholarly journals An instrumented sample holder for time-lapse micro-tomography measurements of snow under advective airflow

2014 ◽  
Vol 4 (1) ◽  
pp. 353-373
Author(s):  
P. P. Ebner ◽  
S. A. Grimm ◽  
M. Schneebeli ◽  
A. Steinfeld
Keyword(s):  
Structural Changes ◽  
Time Lapse ◽  
Sample Holder ◽  
Experimental Characterization ◽  
Computational Fluid Dynamics Simulations ◽  
Micro Tomography ◽  
Dynamics Simulations ◽  
Snow Samples

Abstract. An instrumented sample holder was developed for time-lapse micro-tomography of snow samples to enable in-situ nondestructive spatial and temporal measurements under controlled advective airflows, temperature gradients, and air humidities. The design was aided by computational fluid dynamics simulations to evaluate the airflow uniformity across the snow sample. Morphological and mass transport properties were evaluated during a 4 day test run. This instrument allows the experimental characterization of metamorphism of snow undergoing structural changes with time.

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