Enzyme-linked immunosorbent assay detection and bioactivity of Cry1Ab protein fragments

Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82–24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

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RAPID DIAGNOSIS OF TYPHOID FEVER BY ENZYME-LINKED IMMUNOSORBENT ASSAY DETECTION OF SALMONELLA SEROTYPE TYPHI ANTIGENS IN URINE

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2004.70.323 ◽

2004 ◽

Vol 70 (3) ◽

pp. 323-328 ◽

Cited By ~ 30

Author(s):

MOUSTAFA ABDEL FADEEL ◽

YEHIA SULTAN ◽

DAWLAT EL MELEGI ◽

WILLIAM F. BIBB ◽

BAHEIA REYAD ◽

...

Keyword(s):

Typhoid Fever ◽

Immunosorbent Assay ◽

Rapid Diagnosis ◽

Enzyme Linked Immunosorbent Assay ◽

Salmonella Serotype ◽

Assay Detection

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Enzyme-Linked Immunosorbent Assay Detection of Bacterial Wilt–Causing Ralstonia solanacearum

Springer Protocols Handbooks - Experimental Protocols in Biotechnology ◽

10.1007/978-1-0716-0607-0_1 ◽

2020 ◽

pp. 1-18

Author(s):

Pramila Devi Umrao ◽

Vineet Kumar ◽

Shilpa Deshpande Kaistha

Keyword(s):

Immunosorbent Assay ◽

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Enzyme Linked Immunosorbent Assay ◽

Assay Detection

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Enzyme-Linked Immunosorbent Assay Detection of Melamine in Infant Formula and Wheat Food Products

Journal of Food Protection ◽

10.4315/0362-028x-73.4.701 ◽

2010 ◽

Vol 73 (4) ◽

pp. 701-707 ◽

Cited By ~ 15

Author(s):

ERIC A. E. GARBER ◽

VICKERY A. BREWER

Keyword(s):

Sample Preparation ◽

Infant Formula ◽

Immunosorbent Assay ◽

Cyanuric Acid ◽

Food Products ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

Limits Of Detection ◽

Wheat Products ◽

Assay Detection

The adulteration of food products with melamine to inflate the nitrogen content necessitates the establishment of analytical methods that can distinguish between proteinaceous ingredients and such adulterants. The specificity and ability to detect melamine by two commercial enzyme-linked immunosorbent assay (ELISA) kits were evaluated along with three protocols for sample preparation. Both ELISAs displayed cross-reactivity with ammeline, but neither was able to detect ammelide or cyanuric acid, indicating either a requirement for the 4,6-diamino-1,3,5-triazine structure or inability to bind 1,3,5-triazine-4,6-diones. The limits of detection for melamine in powder infant formula ranged from 0.2 to 3 μg/g depending on the ELISA kit and the method used to prepare the sample. The limits of detection for melamine in liquid infant formula and wheat products were <1 μg/ml and <2.5 μg/g, respectively. The ELISA kits provide an effective alternative for the analysis of samples suspected of containing melamine without relying on extensive sample preparation or expensive instrumentation.

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