Enzyme-Linked Immunosorbent Assay Detection of Bacterial Wilt–Causing Ralstonia solanacearum

2020 ◽  
pp. 1-18
Author(s):  
Pramila Devi Umrao ◽  
Vineet Kumar ◽  
Shilpa Deshpande Kaistha
Keyword(s):  
Immunosorbent Assay ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Assay Detection

Enzyme-linked immunosorbent assay detection and bioactivity of Cry1Ab protein fragments

2016 ◽  
Vol 35 (12) ◽  
pp. 3101-3112 ◽  
Author(s):  
Vurtice C. Albright ◽  
Richard L. Hellmich ◽  
Joel R. Coats
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cry1ab Protein ◽  
Protein Fragments ◽  
Assay Detection

scholarly journals Levels of Airborne Soybean Allergen (Gly m 1) in a Brazilian Soybean Production City: A Pilot Study

2020 ◽  
Vol 17 (15) ◽  
pp. 5381
Author(s):  
Cinthya Covessi Thom de Souza ◽  
Nelson Augusto Rosário Filho ◽  
Juliana Francis de Camargo ◽  
Ricardo Henrique Moreton Godoi
Keyword(s):  
Pilot Study ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Soybean Production ◽  
Soy Production ◽  
Assay Detection ◽  
Soybean Allergen

Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82–24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

RAPID DIAGNOSIS OF TYPHOID FEVER BY ENZYME-LINKED IMMUNOSORBENT ASSAY DETECTION OF SALMONELLA SEROTYPE TYPHI ANTIGENS IN URINE

2004 ◽  
Vol 70 (3) ◽  
pp. 323-328 ◽  
Author(s):  
MOUSTAFA ABDEL FADEEL ◽  
YEHIA SULTAN ◽  
DAWLAT EL MELEGI ◽  
WILLIAM F. BIBB ◽  
BAHEIA REYAD ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Immunosorbent Assay ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Salmonella Serotype ◽  
Assay Detection

scholarly journals Wilt Pathogens of Solanaceae in Tanzania: Clavibacter michiganensis subsp. michiganensis, Pseudomonas corrugata, and Ralstonia solanacearum

Plant Disease ◽  
1999 ◽  
Vol 83 (11) ◽  
pp. 1070-1070 ◽  
Author(s):  
R. Black ◽  
S. Seal ◽  
Z. Abubakar ◽  
R. Nono-Womdim ◽  
I. Swai
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Polymyxin B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Canker ◽  
Clavibacter Michiganensis ◽  
Pseudomonas Corrugata ◽  
Tomato Seed ◽  
Clavibacter Michiganensis Subsp ◽  
Northern Highlands

Surveys of vegetables in the southern and northern growing regions of mainland Tanzania and Zanzibar during 1997 and 1998 indicated the presence of three wilt pathogens of tomato. Clavibacter michiganensis subsp. michiganensis (cause of bacterial canker) was isolated from wilting plants in the southern highlands and Lushoto District (Tanga Region, north) on selective King's medium B with polymyxin B (1). The identity of the isolates was confirmed by cultural, morphological, and biochemical characteristics and a plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) kit (Pathoset 113-08, Adgen, Auchincruive, U.K.). Pathogenicity was confirmed by host inoculation. In addition, the bacterium was detected directly by the same methods in several sources of seed, including commercial farmers' saved seed and seed extracted from infected plants. Of 61 tomato seed lots tested, 18 samples were positive. Ralstonia solanacearum (cause of bacterial wilt) was isolated from tomato and potato by semiselective media and detected directly in stem and tuber tissues, respectively, by polymerase chain reaction and ELISA (4) in all vegetable-growing areas surveyed. In 1998, R. solanacearum was detected for the first time in Zanzibar on tomato and eggplant. Pathogenicity was confirmed by inoculation on and reisolation from tomato seedlings of cv. Money Maker. Only biovar 3 (2) occurred in tomato. Biovar 3 also was found in midaltitude potato. Biovar 2 has been found only in potato plants grown above 1,500 m. Pseudomonas corrugata (cause of pith necrosis) was isolated from tomato on semiselective media at only one location. From wilted tomato plants in the southern highlands, 38% of samples tested positive for C. michiganensis subsp. michiganensis and ≈ 10% for R. solanacearum. Of samples collected from the northern highlands, 43% tested positive for R. solanacearum. Wilt incidence of ≈35% was observed in tomato fields where the bacterial wilt pathogen was isolated in the northern highlands compared with gt;90% incidence and almost total crop loss in tomato fields of the southern highlands infected with bacterial canker. Although all three pathogens caused systemic wilt of plants, bacterial canker occasionally caused downward turning of lower leaves, unilateral wilting, and marginal necrosis of leaflets as well as fruit spotting. In general, wilts caused by C. michiganensis subsp. michiganensis, R. solanacearum, and P. corrugata were not readily differentiated in Tanzania prior to this research. Plants with pith browning had often been assumed to be infected by P. corrugata, until R. solanacearum was isolated (3) frequently from such plants. References: (1) Anonymous. OEPP/EPPO Bull. 22:219, 1992. (2) A. C. Hayward. J. Appl. Bacteriol. 27:265, 1964. (3) J. B. Jones et al., eds. 1991. Compendium of Tomato Diseases. The American Phytopathological Society, St. Paul, MN. (4) S. Seal and J. G. Elphinstone. Pages 35–57 in: Bacterial Wilt. The Disease and Its Causative Agent, Pseudomonas solanacearum. A. C. Hayward and G. L. Hartman, eds. CAB International, Wallingford, U.K. 1994.

Enzyme-Linked Immunosorbent Assay Detection of Melamine in Infant Formula and Wheat Food Products

2010 ◽  
Vol 73 (4) ◽  
pp. 701-707 ◽  
Author(s):  
ERIC A. E. GARBER ◽  
VICKERY A. BREWER
Keyword(s):  
Sample Preparation ◽  
Infant Formula ◽  
Immunosorbent Assay ◽  
Cyanuric Acid ◽  
Food Products ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Limits Of Detection ◽  
Wheat Products ◽  
Assay Detection

The adulteration of food products with melamine to inflate the nitrogen content necessitates the establishment of analytical methods that can distinguish between proteinaceous ingredients and such adulterants. The specificity and ability to detect melamine by two commercial enzyme-linked immunosorbent assay (ELISA) kits were evaluated along with three protocols for sample preparation. Both ELISAs displayed cross-reactivity with ammeline, but neither was able to detect ammelide or cyanuric acid, indicating either a requirement for the 4,6-diamino-1,3,5-triazine structure or inability to bind 1,3,5-triazine-4,6-diones. The limits of detection for melamine in powder infant formula ranged from 0.2 to 3 μg/g depending on the ELISA kit and the method used to prepare the sample. The limits of detection for melamine in liquid infant formula and wheat products were <1 μg/ml and <2.5 μg/g, respectively. The ELISA kits provide an effective alternative for the analysis of samples suspected of containing melamine without relying on extensive sample preparation or expensive instrumentation.

Enzyme-linked immunosorbent assay detection of prostate-specific antigen messenger ribonucleic acid in prostate cancer

Urology ◽  
1999 ◽  
Vol 53 (1) ◽  
pp. 228-235 ◽  
Author(s):  
Senji Hoshi ◽  
Satsuki Kobayashi ◽  
Toshiko Takahashi ◽  
Ken-Ichi Suzuki ◽  
Sadafumi Kawamura ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Immunosorbent Assay ◽  
Ribonucleic Acid ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Messenger Ribonucleic Acid ◽  
Assay Detection
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