Characterization of quality of sediments from Paranaguá Bay (Brazil) by combined in vitro bioassays and chemical analyses

Abstract The development of native-like HIV-1 envelope (Env) trimer antigens has enabled the induction of neutralizing antibody (NAb) responses against neutralization-resistant HIV-1 strains in animal models. However, NAb responses are relatively weak and narrow in specificity. Displaying antigens in a multivalent fashion on nanoparticles (NPs) is an established strategy to increase their immunogenicity. Here we present the design and characterization of two-component protein NPs displaying 20 stabilized SOSIP trimers from various HIV-1 strains. The two-component nature permits the incorporation of exclusively well-folded, native-like Env trimers into NPs that self-assemble in vitro with high efficiency. Immunization studies show that the NPs are particularly efficacious as priming immunogens, improve the quality of the Ab response over a conventional one-component nanoparticle system, and are most effective when SOSIP trimers with an apex-proximate neutralizing epitope are displayed. Their ability to enhance and shape the immunogenicity of SOSIP trimers make these NPs a promising immunogen platform.

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Sensitivity of Colletotrichum truncatum to Four Fungicides and Characterization of Thiabendazole-Resistant Isolates

Plant Disease ◽

10.1094/pdis-11-14-1183-re ◽

2015 ◽

Vol 99 (11) ◽

pp. 1590-1595 ◽

Cited By ~ 9

Author(s):

C. Torres-Calzada ◽

R. Tapia-Tussell ◽

I. Higuera-Ciapara ◽

R. Martin-Mex ◽

A. Nexticapan-Garcez ◽

...

Keyword(s):

Effective Control ◽

Common Disease ◽

Colletotrichum Truncatum ◽

Physic Nut ◽

Fungicide Sensitivity ◽

Tropical Crops ◽

Control Schemes

Anthracnose, caused by Colletotrichum truncatum (syn. C. capsici), has become a common disease of tropical crops, severely affecting the quantity and quality of fruit and seed and, therefore, reducing their market value. For years, chemical control has been extensively used for managing this disease. However, the appearance of isolates that are resistant to the most commonly employed fungicides is increasingly widespread. Twenty C. truncatum isolates from pepper, papaya, and physic nut were tested in vitro against four fungicides to determine their sensitivity. All evaluated isolates were resistant to azoxystrobin and thiabendazole and susceptible to cyprodinil + fludioxonil and mancozeb. To determine the molecular mechanism conferring thiabendazole resistance, the TUB-2 gene was characterized, revealing a glutamic acid to alanine substitution at position 198 in 6 of the 20 isolates that were tested. This work confirms the emergence of benzimidazole-based fungicide resistance in C. truncatum populations and highlights the need for monitoring fungicide sensitivity as an essential activity for the development of effective control schemes.

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Drug-Membrane Interactions Studied in Phospholipid Monolayers Adsorbed on Nonporous Alkylated Microspheres

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057106297063 ◽

2007 ◽

Vol 12 (2) ◽

pp. 186-202 ◽

Cited By ~ 11

Author(s):

Viera Lukacova ◽

Ming Peng ◽

Gail Fanucci ◽

Roman Tandlich ◽

Anne Hinderliter ◽

...

Keyword(s):

Transition Temperature ◽

Partition Coefficients ◽

Temperature Pattern ◽

Drug Candidates ◽

Phospholipid Monolayers ◽

Successful Prediction ◽

Slow Transport

Characterization of interactions with phospholipids is an integral part of the in vitro profiling of drug candidates because of the roles the interactions play in tissue accumulation and passive diffusion. Currently used test systems may inadequately emulate the bilayer core solvation properties (immobilized artificial membranes [IAM]), suffer from potentially slow transport of some chemicals (liposomes in free or immobilized forms), and require a tedious separation (if used for free liposomes). Here the authors introduce a well-defined system overcoming these drawbacks: nonporous octadecylsilica particles coated with a self-assembled phospholipid monolayer. The coating mimics the structure of the headgroup region, as well as the thickness and properties of the hydrocarbon core, more closely than IAM. The monolayer has a similar transition temperature pattern as the corresponding bilayer. The particles can be separated by filtration or a mild centrifugation. The partitioning equilibria of 81 tested chemicals were dissected into the headgroup and core contributions, the latter using the alkane/water partition coefficients. The deconvolution allowed a successful prediction of the bilayer/water partition coefficients with the standard deviation of 0.26 log units. The plate-friendly assay is suitable for high-throughput profiling of drug candidates without sacrificing the quality of analysis or details of the drug-phospholipid interactions.

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Rapid Rubber Extraction and NMR Spectroscopy of Rubber, Extracted from the Endemic Species Scorzonera Tau-Saghyz

Eurasian Chemico-Technological Journal ◽

10.18321/ectj931 ◽

2020 ◽

Vol 22 (1) ◽

pp. 59

Author(s):

K.K. Boguspaev ◽

S.K. Turasheva ◽

T.M. Seilkhanov ◽

D.G. Faleev ◽

M.S. Mutalkhanov ◽

...

Keyword(s):

Adventitious Shoots ◽

Physiological Age ◽

Root Extract ◽

Nmr Characterization ◽

First Time ◽

Rubber Good ◽

Isoprene Rubber

Scorzonera tau-saghyz Lipsch. et G.G. Bosse is an endemic rubber producing plant, growing in mountain regions in South Kazakhstan. The rubber content in plants and the quality of biopolymer has an important impact on industrial rubber production. The results of this study showed that the amount of rubber in S. tau-saghyz roots fluctuates between 7.74% and 38.75%. The amount of synthesized and deposited rubber biopolymer particles depends on various factors such as physiological age of plant, origin, temperature, moisture and environmental conditions. We optimized the extraction method of natural rubber by using n-hexane as a solvent for direct extraction. This method allows extracting the maximum amount of rubber from 3‒4-year-old plants. NMR results show structural links of natural isoprene rubber in the root extract sample. There is a clear relationship between methyl, methine and methylene protons which corresponds to isoprene rubber structure. The samples having strongly marked singlets that are inherent for rubber functional groups confirms the stereospecific structure of rubber. Good solubility of the root extract in deuterated chloroform can characterize the low molecular weight of the polymer. NMR characterization of rubber, extracted from S. tau-saghyz roots, is reported for the first time. Regeneration in vitro provides an important opportunity for endemic preservation by rapidly increasing the number of plants. The best regeneration of adventitious shoots was obtained on MS medium containing 5.5 μM kinetin and 0.5 μM NAA. The plants were successfully acclimatized in a glasshouse with 75% of S. tau-saghyz plantlets, respectively surviving after transfer to ex vitro conditions.

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FORMULATION DEVELOPMENT AND CHARACTERIZATION OF TRIAMCINOLONE LOADED CUBOSOMES FOR TRANSDERMAL DRUG DELIVERY

International Journal of Pharmaceutical Sciences and Medicine ◽

10.47760/ijpsm.2021.v06i12.001 ◽

2021 ◽

Vol 6 (12) ◽

pp. 1-17

Author(s):

C. Pandian ◽

A.Abdul Hasan Sathali ◽

G. Abirami ◽

E. Krithika

Keyword(s):

Transdermal Delivery ◽

In Vitro Release ◽

Poloxamer 407 ◽

Formulation Development ◽

Inflammatory Reactions ◽

Barrier Effects ◽

Low Solubility

Psoriasis is a chronic condition that is caused by the negative signals given by immune system, which leads to hyperproliferation and other inflammatory reactions on the skin. These conditions may adversely affect the quality of the patient’s life leading to psychological stress. Topical delivery of drug is always preferred for Psoriasis because other treatments may lead to systemic intoxication and other adverse reactions. Triamcinolone is a topical corticosteroid belonging to BCS class IV (low solubility and permeability) used to treat Psoriasis. The limitations with transdermal delivery is that only a small amount of the drug can be transferred through the skin tissue due to the barrier effects of the Stratum corneum. Therefore, Novel transdermal delivery system, Cubosomes belonging to Nanostructured lipid carriers were chosen to overcome the issues of solubility and permeability. Twelve formulations were prepared with various ratios of Glyceryl monooleate (2.5 to 5%) & Poloxamer 407 (0.5 to 2%) and the formulations were evaluated for particle size, PDI, zeta potential, entrapment efficacy, drug content and in-vitro release. The best composition of Cubosomes was selected and incorporated into transdermal patch and the formulated patches were evaluated.

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Pharmacological characterization of the bradykinin B2 receptor antagonist MEN16132 in rat in vitro bioassays

European Journal of Pharmacology ◽

10.1016/j.ejphar.2009.04.057 ◽

2009 ◽

Vol 615 (1-3) ◽

pp. 10-16 ◽

Cited By ~ 9

Author(s):

Stefania Meini ◽

Paola Cucchi ◽

Claudio Catalani ◽

Francesca Bellucci ◽

Sandro Giuliani ◽

...

Keyword(s):

Receptor Antagonist ◽

Pharmacological Characterization ◽

Bradykinin B2 Receptor ◽

In Vitro Bioassays

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PPIP5K2 and PCSK1 are Candidate Genetic Contributors to Familial Keratoconus

Scientific Reports ◽

10.1038/s41598-019-55866-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 10

Author(s):

Mariam Lofty Khaled ◽

Yelena Bykhovskaya ◽

Chunfang Gu ◽

Alice Liu ◽

Michelle D. Drewry ◽

...

Keyword(s):

Gene Trap ◽

Whole Exome ◽

The Us ◽

Related Quality ◽

First Time

AbstractKeratoconus (KC) is the most common corneal ectatic disorder affecting >300,000 people in the US. KC normally has its onset in adolescence, progressively worsening through the third to fourth decades of life. KC patients report significant impaired vision-related quality of life. Genetic factors play an important role in KC pathogenesis. To identify novel genes in familial KC patients, we performed whole exome and genome sequencing in a four-generation family. We identified potential variants in the PPIP5K2 and PCSK1 genes. Using in vitro cellular model and in vivo gene-trap mouse model, we found critical evidence to support the role of PPIP5K2 in normal corneal function and KC pathogenesis. The gene-trap mouse showed irregular corneal surfaces and pathological corneal thinning resembling KC. For the first time, we have integrated corneal tomography and pachymetry mapping into characterization of mouse corneal phenotypes which could be widely implemented in basic and translational research for KC diagnosis and therapy in the future.

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