scholarly journals Lytic enzyme production optimization using low‐cost substrates and its application in the clarification of xanthan gum culture broth

2014 ◽  
Vol 2 (4) ◽  
pp. 299-307 ◽  
Author(s):  
Cíntia Reis Silva ◽  
Marilia Lordelo Cardoso Silva ◽  
Helio Mitoshi Kamida ◽  
Aristoteles Goes‐Neto ◽  
Maria Gabriela Bello Koblitz
Keyword(s):  
Enzyme Production ◽  
Xanthan Gum ◽  
Low Cost ◽  
Culture Broth ◽  
Production Optimization ◽  
Lytic Enzyme

HYPER-PRODUCTION OPTIMIZATION OF FUNGAL OXIDATIVE GREEN ENZYMES USING CITRUS LOW-COST BYPRODUCT

2020 ◽  
pp. 105013
Author(s):  
Débora S. Vilar ◽  
Clara D. Fernandes ◽  
Victor R.S. Nascimento ◽  
Nádia H. Torres ◽  
Manuela S. Leite ◽  
...  
Keyword(s):  
Low Cost ◽  
Production Optimization

scholarly journals Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

2020 ◽  
Vol 12 (17) ◽  
pp. 7122
Author(s):  
Ludwika Tomaszewska-Hetman ◽  
Waldemar Rymowicz ◽  
Anita Rywińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Culture Broth ◽  
Biodiesel Production ◽  
Innovative Strategy ◽  
Simple Medium ◽  
Low Cost Strategy ◽  
Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

scholarly journals Fusarium concolor X4 Pretreatment Suppresses Light-Induced Yellowing of High-Yield Pulp

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Daolei Zhang ◽  
Xuezhi Li ◽  
Jian Zhao
Keyword(s):  
Absorption Spectra ◽  
Enzyme Production ◽  
Low Cost ◽  
High Yield ◽  
Inhibition Mechanism ◽  
Chemical Components ◽  
Mechanical Pulp ◽  
Brightness Reversion ◽  
Before And After ◽  
Wood Utilization

High-yield pulps (HYPs), such as CTMP (chemi-thermo-mechanical pulp), are attractive due to their low cost and high wood utilization. However, their drawback of rapid brightness reversion (yellowing) limits wide use of the HYPs. In this study, a fungus, Fusarium concolor X4, was applied to treat poplar CTMP for exploring the effects of biotreatment on brightness and light-induced yellowing of the pulp. The results indicated that the biotreatment with Fusarium concolor X4 could improve the brightness of poplar CTMP and inhibit light-induced yellowing of the pulp. The yellowing inhibition mechanism was explored by the analysis of enzyme production regularity during biotreatment, changes in chemical components, and the UV-Vis absorption spectra and FTIR-ATR spectra of pulps before and after biotreatment.

scholarly journals Novel Application of Mahua (Madhuca sp.) Flowers for Augmented Protease Production from Aeromonas sp. S1

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Amrik Bhattacharya ◽  
Vandana Saini ◽  
Anshu Gupta
Keyword(s):  
Enzyme Production ◽  
Low Cost ◽  
Inoculum Size ◽  
Protease Production ◽  
Agitation Rate ◽  
Value Addition ◽  
New Approach ◽  
Flower Extract ◽  
Optimized Conditions ◽  
Optimum Production

The present study explored the utilization of Mahua ( Madhuca sp.) flowers, a major non-timber forest product (NTFP) of India, as a low-cost, natural substrate for protease production under submerged fermentation. Bacterial strain Aeromonas sp. S1, previously reported by us, was used as the protease producer. Using Mahua flower extract (MFE) as the medium additive, the protease production could successfully be enhanced by 5.6-fold (564.5 UmL−1) after 24 h of fermentation under optimized conditions compared with initial production of 99.9 UmL−1 in the absence of MFE. The cultural parameters for optimum production of protease were determined to be: incubation time-24 h; pH-7.0; MFE concentration-5% (v/v); inoculum size-0.3% (v/v) and agitation rate-200 rpm. The results obtained demonstrate the potential of cheaper and abundantly available Mahua flowers for induction of proteases, and thus offer a new approach for value addition to this biomass through industrial enzyme production.

scholarly journals Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic‐enzyme production in L ysobacter enzymogenes   OH 11

2015 ◽  
Vol 8 (3) ◽  
pp. 499-509 ◽  
Author(s):  
Gaoge Xu ◽  
Yuxin Zhao ◽  
Liangcheng Du ◽  
Guoliang Qian ◽  
Fengquan Liu
Keyword(s):  
Enzyme Production ◽  
Lytic Enzyme ◽  
Antibiotic Biosynthesis

scholarly journals Enzyme fatigue limits the detoxification of aflatoxin by Rhodococcus species

2021 ◽  
Author(s):  
Marco Zaccaria ◽  
Natalie Sandlin ◽  
David H Fu ◽  
Marek Domin ◽  
Babak Momeni
Keyword(s):  
Enzyme Production ◽  
Extracellular Enzymes ◽  
Low Cost ◽  
Fungal Growth ◽  
Quantitative Approach ◽  
Live Cells ◽  
Environmental Isolates ◽  
Environment Friendly ◽  
Rhodococcus Species ◽  
Food Commodities

Bacterial detoxification of mycotoxins has the potential to offer a low-cost solution to ensure that feed and food commodities contaminated by fungal growth become safe to consume. Among bacteria, Rhodococcus species are of particular interest because they can be metabolically versatile, non-pathogenic, and environment-friendly. However, the native response of Rhodococcus environmental isolates appears inadequate for current detoxification needs. By analyzing the detoxification of aflatoxin by two Rhodococcus species: R. pyridinivorans and R. erythropolis, we examine important features of the dynamics that could guide future optimization of bacterial detoxification. Our results for Rhodococcus species suggest that detoxification happens through a regulated process of secreting extracellular enzymes. We show that enzyme fatigue in the presence of the toxin determines the lifetime of the enzyme and limits the overall detoxification performance of these species. Additionally, we show that the regulation of enzyme production can be both species- and environment-dependent. Overall, our quantitative approach reveals that enzyme fatigue is a major determinant of overall detoxification and needs to be accounted for in assessing the performance of detoxification by live cells or cell-free filtrates.

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