Decision letter for "<i>In Vitro</i> Maturation is a viable option for urgent fertility preservation in young women with hematological conditions"

AbstractWe report a case of ultrasound-guided ex vivo oocyte retrieval for fertility preservation in a woman with bilateral borderline ovarian tumor, for whom conventional transvaginal oocyte retrieval was deemed unsafe because of the increased risk of malignant cell spillage. Ovarian stimulation with gonadotropins was performed. Surgery was scheduled according to the ovarian response to exogenous gonadotropic stimulation; oophorectomized specimens were obtained by laparoscopy, and oocyte retrieval was performed ∼ 37 hours after the ovulatory trigger. The sum of 20 ovarian follicles were aspirated, and 16 oocytes were obtained. We performed vitrification of 12 metaphase II oocytes and 3 oocytes matured in vitro. Our result emphasizes the viability of ex vivo mature oocyte retrieval after controlled ovarian stimulation for those with high risk of malignant dissemination by conventional approach.

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Pediatric and Adolescent Oncofertility in Male Patients—From Alpha to Omega

Genes ◽

10.3390/genes12050701 ◽

2021 ◽

Vol 12 (5) ◽

pp. 701

Author(s):

Ovidiu Bîcă ◽

Ioan Sârbu ◽

Carmen Iulia Ciongradi

Keyword(s):

Fertility Preservation ◽

Gene Editing ◽

Genetic Diseases ◽

Reproductive Potential ◽

Molecular Techniques ◽

Male Population ◽

Male Survivors ◽

Male Patients ◽

Survivors Of Childhood Cancer

This article reviews the latest information about preserving reproductive potential that can offer enhanced prospects for future conception in the pediatric male population with cancer, whose fertility is threatened because of the gonadotoxic effects of chemotherapy and radiation. An estimated 400,000 children and adolescents aged 0–19 years will be diagnosed with cancer each year. Fertility is compromised in one-third of adult male survivors of childhood cancer. We present the latest approaches and techniques for fertility preservation, starting with fertility preservation counselling, a clinical practice guideline used around the world and finishing with recent advances in basic science and translational research. Improving strategies for the maturation of germ cells in vitro combined with new molecular techniques for gene editing could be the next scientific keystone to eradicate genetic diseases such as cancer related mutations in the offspring of cancer survivors.

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Priming with a gonadotropin-releasing hormone agonist before immature oocyte retrieval may improve maturity of oocytes and outcome in in vitro maturation (IVM) cycle: a case report

Journal of Medical Case Reports ◽

10.1186/s13256-021-02706-8 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

A. Smirnova ◽

M. Anshina ◽

E. Shalom Paz ◽

A. Ellenbogen

Keyword(s):

Fertility Preservation ◽

Chorionic Gonadotropin ◽

Human Chorionic Gonadotropin ◽

Oocyte Retrieval ◽

Fertilization Rate ◽

Gonadotropin Releasing Hormone ◽

Releasing Hormone ◽

Nuclear Maturation ◽

Gonadotropin Releasing Hormone Agonist

Abstract Background The concept of using a gonadotropin-releasing hormone agonist (GnRH-a) instead of human chorionic gonadotropin for triggering ovulation in patients treated with an antagonist protocol for in vitro fertilization (IVF) has become a routine clinical practice. It may promote oocyte nuclear maturation, resumption of meiosis and cumulus expansion. It seems that this attempt could be beneficial in an in vitro maturation (IVM) oocyte cycle performed for polycystic ovarian syndrome as well as for other indications such as urgent fertility preservation in patients with malignancies or unusual indications. Case presentation We present the case of a Caucasian patient who needed fertility preservation when routine natural IVF treatment did not yield oocyte retrieval, followed by three IVM cycles, priming ovulation with a GnRH-a. In total, 12 oocytes were obtained, all matured 4.5 hours after incubation in maturation media. The fertilization rate after intracytoplasmic sperm injection was 83%. Six good-quality embryos were vitrified. Conclusions It seems that triggering with a GnRH-a in selected cases may replace human chorionic gonadotropin in IVM of oocytes and could be highly beneficial in terms of obtaining high-grade embryos and possible pregnancy.

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Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽

10.1017/s0967199420000696 ◽

2020 ◽

pp. 1-3

Author(s):

Burcu Ozbakir ◽

Pinar Tulay

Keyword(s):

Alcohol Consumption ◽

Young Women ◽

Antral Follicle ◽

Antral Follicle Count ◽

Control Group ◽

Social Drinkers ◽

Antral Follicles ◽

Healthy Women ◽

Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

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