With the emergence of glyphosate-tolerant cultivars, identifying management strategies not applicable with older cultivars need to be revisited. Objectives of these research trials were to quantify the growth regulation effects following a glyphosate application and to determine the safety of tank mixing glyphosate with another herbicide, various nitrogen (N) sources, and a plant growth regulator (PGR) on a glyphosate-tolerant perennial ryegrass [PRG (Lolium perenne L.)] cultivar, Replay. In the growth regulation trial, glyphosate was applied at 0 to 1.03 lb/acre, whereas PGRs flurpimidol, trinexapac-ethyl, paclobutrazol, and trinexapac-ethyl + flurpimidol were applied at 0.50, 0.18, 0.37, and 0.09 + 0.22 lb/acre, respectively, on 15 July 2010 and 2 Aug. 2012. In the tank mixing trial, dicamba (0.50 lb/acre), urea (15 lb/acre N), and ammonium sulfate [AMS (15 lb/acre N)] were applied alone or tank mixed with glyphosate at 0 to 0.52 lb/acre. Tank mixing urea with glyphosate had minimal effect on PRG color, while adding AMS consistently improved color at the highest glyphosate rate of 0.52 lb/acre. Twenty days following a glyphosate application, only rates >0.40 lb/acre resulted in significant growth regulation compared with untreated plots. This study indicates that tank mixing glyphosate with another herbicide, a PGR, and various N sources appear safe to the glyphosate-tolerant PRG cultivar. Also, the growth regulating effects of glyphosate applications would serve as an additional benefit to annual bluegrass (Poa annua L.) control reported in previous trials.