A harmonized and standardized in vitro approach produces reliable results on silver nanoparticles toxicity in different cell lines

2021 ◽  
Author(s):  
Cristina Andreoli ◽  
Valentina Prota ◽  
Isabella De Angelis ◽  
Emiliano Facchini ◽  
Andrea Zijno ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Nanoparticles Toxicity

scholarly journals A Bottom-Up Synthesis Approach to Silver Nanoparticles Induces Anti-Proliferative and Apoptotic Activities Against MCF-7, MCF-7/TAMR-1 and MCF-10A Human Breast Cell Lines

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4332
Author(s):  
Nurul Izzati Zulkifli ◽  
Musthahimah Muhamad ◽  
Nur Nadhirah Mohamad Zain ◽  
Wen-Nee Tan ◽  
Noorfatimah Yahaya ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Leaf Extract ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Human Breast ◽  
Bottom Up ◽  
Mcf 7

A bottom-up approach for synthesizing silver nanoparticles (AgNPs-GA) phytomediated by Garcinia atroviridis leaf extract is described. Under optimized conditions, the AgNPs-GA were synthesized at a concentration of 0.1 M silver salt and 10% (w/v) leaf extract, 1:4 mixing ratio of reactants, pH 3, temperature 32 °C and 72 h reaction time. The AgNPs-GA were characterized by various analytical techniques and their size was determined to be 5–30 nm. FTIR spectroscopy indicates the role of phenolic functional groups in the reduction of silver ions into AgNPs-GA and in supporting their subsequent stability. The UV-Visible spectrum showed an absorption peak at 450 nm which reflects the surface plasmon resonance (SPR) of AgNPs-GA and further supports the stability of these biosynthesized nanoparticles. SEM, TEM and XRD diffractogram analyses indicate that AgNPs-GA were spherical and face-centered-cubic in shape. This study also describes the efficacy of biosynthesized AgNPs-GA as anti-proliferative agent against human breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Our findings indicate that AgNPs-GA possess significant anti-proliferative effects against both the MCF-7 and MCF-7/TAMR-1 cell lines, with inhibitory concentration at 50% (IC50 values) of 2.0 and 34.0 µg/mL, respectively, after 72 h of treatment. An induction of apoptosis was evidenced by flow cytometry using Annexin V-FITC and propidium iodide staining. Therefore, AgNPs-GA exhibited its anti-proliferative activity via apoptosis on MCF-7 and MCF-7/TAMR-1 breast cancer cells in vitro. Taken together, the leaf extract from Garcinia atroviridis was found to be highly capable of producing AgNPs-GA with favourable physicochemical and biological properties.

scholarly journals Octreotide-conjugated silver nanoparticles for active targeting of somatostatin receptors and their application in a nebulized rat model

2021 ◽  
Vol 11 (1) ◽  
pp. 266-283
Author(s):  
Ahmed A. H. Abdellatif ◽  
Riaz A. Khan ◽  
Ahmad H. Alhowail ◽  
Abdulmajeed Alqasoumi ◽  
Sultan M. Sajid ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Somatostatin Receptors ◽  
Somatostatin Analogs ◽  
Drug Uptake ◽  
Emission Spectrometry ◽  
Dependent Manner

Abstract Drug uptake and distribution through cell–receptor interactions are of prime interest in reducing the adverse effects and increasing the therapeutic effectiveness of delivered formulations. This study aimed to formulate silver nanoparticles (AgNPs) conjugated to somatostatin analogs for specific delivery through somatostatin receptors (SSTRs) expressed on cells and by nebulizing the prepared AgNPs formulations into lung cells for in vivo application. AgNPs were prepared using the citrate reduction method, yielding AgNPs–CTT, which was further chemically conjugated to octreotide (OCT) to form AgNPs–OCT through an amide linkage. The AgNPs–OCT formulation was coated using alginate to yield a carrier, AgNPs–OCT–Alg, feasible for drug delivery through nebulization. AgNPs were uniform in size with an acceptable range of zeta potential. Furthermore, the concentrations of AgNP formulations were found safe for the model cell lines used, and cell proliferation was significantly reduced in a dose-dependent manner (p < 0.05). In the healthy lung tissues, AgNPs–OCT–Alg accumulated at a concentration of 0.416 ± 5.7 mg/kgtissue, as determined via inductively coupled plasma optical emission spectrometry. This study established the accumulation of AgNPs, specifically the AgNPs–OCT–Alg, in lung tissues, and substantiated the active, specific, and selective targeting of SSTRs at pulmonary sites. The anticancer efficacy of the formulations was in vitro tested and confirmed in the MCF-7 cell lines. Owing to the delivery suitability and cytotoxic effects of the AgNPs–OCT–Alg formulation, it is a potential drug delivery formulation for lung cancer therapy in the future.

In vitro biological activity of synthesized silver nanoparticles using Myrtus extract

NanoNEXT ◽  
2021 ◽  
pp. 8-19
Author(s):  
Neda Mohamadi ◽  
Mohsen Doostmohammadi ◽  
Iraj Sharifi ◽  
Mehdi Bamorovat ◽  
Ahmad Khosravi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Human Cancer ◽  
Negative Control ◽  
Ros Production ◽  
Bacterial Cells ◽  
Human Cancer Cells ◽  
Ic50 Values ◽  
Mcf 7

This study aimed to synthesize and characterize silver nanoparticles (AgNPs) from M. communis laves, and determine their potential activity against human cancer cells as well as leishmanial and bacterial cells. The UV-visible spectroscopy showed an absorption peak at 430 nm wavelengths which is one of the characteristic features of AgNPs. The FESEM image showed irregular shape with a size range of 20-70 nm. MTT results in A172 and MCF-7 cell lines exposed to 5-240 g/mL for 48 hours revealed that M. communis-AgNPs were cytotoxic, with IC50 values of 93.2 g/mL for A172 cell lines and 89.1 g/mL for MCF-7 cell lines, respectively. DCFH-DA analysis showed that 24 h exposure to 25- 200 μg/mL concentrations of AgNPs significantly increased ROS production in cells that indicate oxidative stress induction by AgNPs. M. communis-AgNPs showed overexpression of BCL-2 and Bax genes compared with Glucantime®and negative control (p<0.001) as a potent leishmanicidal and bactericidal activity. The primary modes of action seem to be involved by promotion of the ROS production and up-regulation of BCL-2 and Bax against cancer cell lines. As a result, M. communis-AgNPs formulation should be regarded as a promising agent for potential anti-cancer, anti-leishmanial, and anti-bacterial drugs in therapeutic control programs

Potential in-vitro anti-breast cancer activity of green-synthesized silver nanoparticles preparation against human MCF-7 cell-lines

2019 ◽  
Vol 10 (4) ◽  
pp. 045012 ◽  
Author(s):  
Riazunnisa Khateef ◽  
Habeeb Khadri ◽  
Ahmad Almatroudi ◽  
Sultan A Alsuhaibani ◽  
S Anjum Mobeen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cell Lines ◽  
Green Synthesized Silver Nanoparticles ◽  
Cancer Activity ◽  
Mcf 7

Comparative in vitro cytotoxicity of citrate-coated silver nanoparticles on skin, liver and blood cell lines

2016 ◽  
Vol 258 ◽  
pp. S262 ◽  
Author(s):  
V. Bastos ◽  
J. Carrola ◽  
I.F. Duarte ◽  
C. Santos ◽  
H. Oliveira
Keyword(s):  
Silver Nanoparticles ◽  
Blood Cell ◽  
Cell Lines ◽  
In Vitro Cytotoxicity

In-vitro anti-cancer activity of shape controlled silver nanoparticles (AgNPs) in various organ specific cell lines

2017 ◽  
Vol 242 ◽  
pp. 757-766 ◽  
Author(s):  
Sabina Yeasmin ◽  
Hemanta Kumar Datta ◽  
Sujata Chaudhuri ◽  
Debasish Malik ◽  
Abhijit Bandyopadhyay
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Specific Cell ◽  
Anti Cancer ◽  
Organ Specific ◽  
Shape Controlled ◽  
Cancer Activity
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