Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis

Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance. Keywords: Antifungal Agents; Biofilms; Candida; Scanning Electron Microscope; Soft Liners; Hardness; Surface Roughness; Minimal Inhibitory Concentration; Denture Stomatitis.

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Antifungal Susceptibility of Non-albicans Candida Species in A Tertiary Care Hospital, Bulgaria

Jundishapur Journal of Microbiology ◽

10.5812/jjm.101767 ◽

2020 ◽

Vol 13 (8) ◽

Author(s):

Hristina Yotova Hitkova ◽

Diana Simeonova Georgieva ◽

Preslava Mihailova Hristova ◽

Teodora Vasileva Marinova-Bulgaranova ◽

Biser Kirilov Borisov ◽

...

Keyword(s):

Amphotericin B ◽

Antifungal Agents ◽

Antifungal Susceptibility ◽

Tertiary Care ◽

Acquired Resistance ◽

Multidrug Resistant ◽

Antifungal Drugs ◽

Care Hospital ◽

And Control

Background: Emerging non-albicans Candida (NAC) species are a major threat because of their intrinsic or acquired resistance to routinely applied antifungal agents. Objectives: The purpose of our study was to reveal in vitro activity of nine antifungal agents against NAC isolates. Methods: A total of 67 NAC (27 Candida glabrata, 10 C. tropicalis, 6 C. krusei, 6 C. parapsilosis, 4 C. lusitaniae, 4 C. lipolytica, etc.) were identified and tested. The antifungal susceptibility was estimated on the basis of minimum inhibitory concentrations (MIC). Results: Overall, 13 species were determined, of which C. glabrata was the most common (40.3%), followed by C. tropicalis (14.9%), C. krusei, and C. parapsilosis (8.9 % each). Forty-nine NAC isolates (73.13%) demonstrated decreased susceptibility to one or more antifungals, and 18 of them were resistant to all azoles. Out of 27 C. glabrata, 12 (44.4%) were resistant to fluconazole with MICs: 32 - >128 µg/mL and 15 (55.6%) were intermediate with MICs: 8 - 16 µg/mL Non-albicans Candida revealed a good susceptibility to echinocandins. Amphotericin B resistance was found in 5.97% of the isolates. Of particular interest was the detection of 6 (8.95%) multidrug-resistant NAC, which expressed resistance to azoles and echinocandins and/or amphotericin B. Conclusions: About one-fourth of the studied NAC were resistant to all azoles. These findings as well as the detection of several multidrug-resistant isolates determine the necessity of susceptibility testing of clinically important yeast isolates and control of the antifungal drugs in our hospital.

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In Vitro Activities of New Triazole Antifungal Agents, Posaconazole and Voriconazole, Against Oral Candida Isolates from Patients Suffering from Denture Stomatitis

Mycopathologia ◽

10.1007/s11046-011-9460-4 ◽

2011 ◽

Vol 173 (1) ◽

pp. 35-46 ◽

Cited By ~ 16

Author(s):

Cristina Marcos-Arias ◽

Elena Eraso ◽

Lucila Madariaga ◽

Alfonso Javier Carrillo-Muñoz ◽

Guillermo Quindós

Keyword(s):

Antifungal Agents ◽

Denture Stomatitis ◽

Oral Candida

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In vitro efficacy of topical antifungal agents on Malassezia species.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.53.144 ◽

1991 ◽

Vol 53 (1) ◽

pp. 144-151 ◽

Cited By ~ 3

Author(s):

Mamoru YOKOO ◽

Tadashi ARIKA ◽

Yoshiro SOH

Keyword(s):

Antifungal Agents ◽

Malassezia Species

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EDTA inhibits Rhizopus oryzae (Rhizopus arrhizus) growth in vitro in blood of diabetic and non-diabetic individuals - is it an option for combined therapy with antifungal agents in the future?

10.26226/morressier.56d5ba2fd462b80296c96a8e ◽

2016 ◽

Author(s):

Grace Salazar

Keyword(s):

Antifungal Agents ◽

Rhizopus Oryzae ◽

Combined Therapy ◽

Rhizopus Arrhizus ◽

The Future

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DILLIDUR 450V

Alloy Digest ◽

10.31399/asm.ad.sa0638 ◽

2011 ◽

Vol 60 (12) ◽

Keyword(s):

Fracture Toughness ◽

Wear Resistance ◽

Physical Properties ◽

Tensile Properties ◽

Room Temperature ◽

Surface Hardness ◽

Heat Treating ◽

Hot Working ◽

Resistant Steel ◽

Bend Strength

Abstract Dillidur 450V is a water hardened wear-resistant steel with surface hardness at room temperature of 420-480 HB. The steel is easy to weld and bend. Hot working is not recommended. This datasheet provides information on composition, physical properties, hardness, tensile properties, and bend strength as well as fracture toughness. It also includes information on wear resistance as well as forming, heat treating, machining, and joining. Filing Code: SA-638. Producer or source: Dillinger Hütte GTS.

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In vitro diagnostic medical devices. Measurement of quantities in biological samples. Metrological traceability of values for catalytic concentration of enzymes assigned to calibrators and control materials

10.3403/02874056u ◽

2015 ◽

Cited By ~ 1

Keyword(s):

Medical Devices ◽

Biological Samples ◽

Metrological Traceability ◽

In Vitro Diagnostic ◽

And Control

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Faculty Opinions recommendation of In vitro activities of new and conventional antifungal agents against clinical Scedosporium isolates.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1003761.39662 ◽

2002 ◽

Author(s):

Ana Espinel-Ingroff

Keyword(s):

Antifungal Agents

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Synthesis and Antifungal Activity of Coumarins Derivatives Against Sporothrix spp.

Current Topics in Medicinal Chemistry ◽

10.2174/1568026618666180221115508 ◽

2018 ◽

Vol 18 (2) ◽

pp. 164-171 ◽

Cited By ~ 4

Author(s):

Luana da S.M. Forezi ◽

Luana Pereira Borba-Santos ◽

Mariana F.C. Cardoso ◽

Vitor F. Ferreira ◽

Sonia Rozental ◽

...

Keyword(s):

Antifungal Activity ◽

Antifungal Agents ◽

Sporothrix Schenckii ◽

Public Health Problem ◽

Domestic Animals ◽

Coumarin Derivatives ◽

Sporothrix Brasiliensis ◽

Synthetic Routes ◽

Effective Drugs

Sporotrichosis is a serious public health problem in Brazil that affects human patients and domestic animals, mainly cats. Thus, the search for new antifungal agents is required also due to the emergence and to the lack of effective drugs available in the therapeutic arsenal. The aim of this study was to evaluate the in vitro antifungal profile of two synthetic series of coumarin derivatives against Sporothrix schenckii and Sporothrix brasiliensis. The three-components synthetic routes used for the preparation of coumarin derivatives have proved to be quite efficient and compounds 16 and 17 have been prepared in good yields. The inhibitory activity of nineteen synthetic coumarins derivatives 16a-i and 17a-j were evaluated against Sporothrix spp. yeasts and the most potent compounds were 16b and 17i. However, according to concentrations able to inhibit (minimum inhibitory concentrations) and kill (minimum fungicidal concentrations) the cells, 17i was more effective than 16b against Sporothrix spp. Thus, 17i exhibited good antifungal activity against S. brasiliensis and S. schenckii, suggesting that it is an important scaffold for the development of novel antifungal agents.

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Potential of Nanoparticles in Combating Candida Infections

Letters in Drug Design & Discovery ◽

10.2174/1570180815666181015145224 ◽

2019 ◽

Vol 16 (5) ◽

pp. 478-491 ◽

Cited By ~ 5

Author(s):

Faizan Abul Qais ◽

Mohd Sajjad Ahmad Khan ◽

Iqbal Ahmad ◽

Abdullah Safar Althubiani

Keyword(s):

Targeted Delivery ◽

Recent Progress ◽

Antifungal Agents ◽

Fungal Infections ◽

Fold Increase ◽

Antifungal Drugs ◽

Low Toxicity ◽

Candida Infections ◽

Made In

Aims: The aim of this review is to survey the recent progress made in developing the nanoparticles as antifungal agents especially the nano-based formulations being exploited for the management of Candida infections. Discussion: In the last few decades, there has been many-fold increase in fungal infections including candidiasis due to the increased number of immunocompromised patients worldwide. The efficacy of available antifungal drugs is limited due to its associated toxicity and drug resistance in clinical strains. The recent advancements in nanobiotechnology have opened a new hope for the development of novel formulations with enhanced therapeutic efficacy, improved drug delivery and low toxicity. Conclusion: Metal nanoparticles have shown to possess promising in vitro antifungal activities and could be effectively used for enhanced and targeted delivery of conventionally used drugs. The synergistic interaction between nanoparticles and various antifungal agents have also been reported with enhanced antifungal activity.

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