Real time noninvasive monitoring of contaminating bacteria in a soft tissue implant infection model

Virtual surgery robot can accurately modeling of surgical instruments and human organs, and realistic simulation of various surgical phenomena such as deformation of organic tissues, surgery simulation system can provide operators with reusable virtual training and simulation environment. To meet the requirement of virtual surgery robot for the authenticity and real-time of soft tissue deformation and surgical simulation in liver surgery, a new method is proposed to simulate the deformation of soft tissue. This method combines the spring force, the external force of the system, and the constraint force produced by the constraint function of the position-based dynamics. Based on the position-based dynamics, an improved three-parameter mass-spring model is added. In the calculation of the elastic force, the nonlinearity and viscoelasticity of the soft tissue are introduced, and the joint force of the constraint projection process and the constraint force of the position-based dynamics is used to modify mass points movement. The method of position-based dynamics based on biological characteristics, not only considers the biomechanical properties of biological soft tissue as an organic polymer such as viscoelasticity, nonlinearity, and incompressibility but also retains the rapidity and stability of the position based dynamic method. Through the simulation data, the optimal side length of tetrahedral mesh in the improved three-parameter model is obtained, and the physical properties of the model are proved. The real-time simulation of the liver and other organs is completed by using the Geomagic touch force feedback device, which proves the practicability and effectiveness of this method.

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A NASBA on microgel-tethered molecular-beacon microarray for real-time microbial molecular diagnostics

The Analyst ◽

10.1039/c6an02192a ◽

2017 ◽

Vol 142 (1) ◽

pp. 147-155 ◽

Cited By ~ 10

Author(s):

Y. Ma ◽

X. Dai ◽

T. Hong ◽

G. B. Munk ◽

M. Libera

Keyword(s):

Real Time ◽

Bloodstream Infection ◽

Molecular Diagnostics ◽

Molecular Beacon ◽

Molecular Beacons ◽

Infection Model ◽

Rna Amplification ◽

Microbial Detection

Gel-tethered molecular beacons coupled with NASBA RNA amplification enable real-time microbial detection and differentiation in a bloodstream infection model.

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2021 Frank Stinchfield Award: A novel cemented hip hemiarthroplasty infection model with real-time in vivo imaging in rats

The Bone & Joint Journal ◽

10.1302/0301-620x.103b7.bjj-2020-2435.r1 ◽

2021 ◽

Vol 103-B (7 Supple B) ◽

pp. 9-16

Author(s):

William J. Hadden ◽

Mazen Ibrahim ◽

Mariam Taha ◽

Kerstin Ure ◽

Yun Liu ◽

...

Keyword(s):

Real Time ◽

In Vivo Imaging ◽

Biofilm Formation ◽

Joint Infection ◽

Volumetric Analysis ◽

In Vivo Model ◽

Infection Model ◽

Sprague Dawley ◽

Hip Hemiarthroplasty

Aims The aims of this study were to develop an in vivo model of periprosthetic joint infection (PJI) in cemented hip hemiarthroplasty, and to monitor infection and biofilm formation in real-time. Methods Sprague-Dawley rats underwent cemented hip hemiarthroplasty via the posterior approach with pre- and postoperative gait assessments. Infection with Staphylococcus aureus Xen36 was monitored with in vivo photoluminescent imaging in real-time. Pre- and postoperative gait analyses were performed and compared. Postmortem micro (m) CT was used to assess implant integration; field emission scanning electron microscopy (FE-SEM) was used to assess biofilm formation on prosthetic surfaces. Results All animals tolerated surgery well, with preservation of gait mechanics and weightbearing in control individuals. Postoperative in vivo imaging demonstrated predictable evolution of infection with logarithmic signal decay coinciding with abscess formation. Postmortem mCT qualitative volumetric analysis showed high contact area and both cement-bone and cement-implant interdigitation. FE-SEM revealed biofilm formation on the prosthetic head. Conclusion This study demonstrates the utility of a new, high-fidelity model of in vivo PJI using cemented hip hemiarthroplasty in rats. Inoculation with bioluminescent bacteria allows for non-invasive, real-time monitoring of infection. Cite this article: Bone Joint J 2021;103-B(7 Supple B):9–16.

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Real-time needle shape prediction in soft-tissue based on image segmentation and particle filtering

2016 IEEE International Conference on Advanced Intelligent Mechatronics (AIM) ◽

10.1109/aim.2016.7576934 ◽

2016 ◽

Cited By ~ 5

Author(s):

Jay Carriere ◽

Carlos Rossa ◽

Ronald Sloboda ◽

Nawaid Usmani ◽

Mahdi Tavakoli

Keyword(s):

Image Segmentation ◽

Soft Tissue ◽

Real Time ◽

Particle Filtering ◽

Shape Prediction

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Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis

Journal of Medical Microbiology ◽

10.1099/jmm.0.008169-0 ◽

2009 ◽

Vol 58 (5) ◽

pp. 648-655 ◽

Cited By ~ 39

Author(s):

Kristel Lourdault ◽

Florence Aviat ◽

Mathieu Picardeau

Keyword(s):

Guinea Pig ◽

Real Time ◽

Real Time Pcr ◽

Guinea Pigs ◽

Infection Model ◽

Avirulent Strain ◽

Leptospira Interrogans ◽

Quantitative Real Time Pcr ◽

Guinea Pig Model

The dynamics of leptospirosis infection have been poorly studied. The purpose of this study was to determine the LD50, rate of bacterial dissemination, histopathology and antibody responses against leptospira following inoculation with the highly virulent Leptospira interrogans Fiocruz L1-130 strain in a guinea pig model of leptospirosis. Three routes of infection (intraperitoneal, conjunctival and subcutaneous inoculation) were used to establish disease in guinea pigs. The size and kinetics of leptospiral burdens in the blood and tissues of infected animals were determined over a 1 week course of infection using quantitative real-time PCR (qPCR). Bacteraemia peaked at day 5 post-infection reaching more than 5×104 leptospires ml−1. The highest spirochaetal load was found in the liver and kidneys, and was associated with alterations in organ tissues and a decline in liver and kidney functions. In contrast, lesions and bacteria were not detected in guinea pigs infected with an avirulent strain derived from a high-passage-number in vitro-passaged variant of the Fiocruz L1-130 strain. The use of qPCR supports the findings of earlier studies and provides an easy and reliable method for the quantification of L. interrogans in the tissues of infected animals. qPCR will be used in future studies to evaluate the efficacy of vaccine candidates against leptospirosis and the virulence of selected L. interrogans mutants relative to the parental strain.

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