scholarly journals A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1561
Author(s):  
Liubov Safonova ◽  
Maria Bobrova ◽  
Anton Efimov ◽  
Alexey Lyundup ◽  
Olga Agapova ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Silk Fibroin ◽  
Biological Properties ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Thickness Skin ◽  
Internal Structures ◽  
Wound Healing Model ◽  
Healing Model

A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.

scholarly journals Silk Fibroin/Spidroin Electrospun Scaffolds for Full-Thickness Skin Wound Healing in Rats

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1704
Author(s):  
Liubov Safonova ◽  
Maria Bobrova ◽  
Anton Efimov ◽  
Lyubov Davydova ◽  
Timur Tenchurin ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Fiber Surface ◽  
Volume Ratio ◽  
Biological Properties ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Electrospun Scaffolds ◽  
Wound Model ◽  
Volume Porosity

The main goal of our research was to fabricate electrospun scaffolds from three different silk proteins—silk fibroin from Bombyx mori silkworm cocoons and two recombinant spidroins, rS2/12 and rS2/12-RGDS—and to perform a comparative analysis of the structure, biological properties, and regenerative potential of the scaffolds in a full-thickness rat skin wound model. The surface and internal structures were investigated using scanning electron microscopy and scanning probe nanotomography. The structures of the scaffolds were similar. The average fiber diameter of the scaffolds was 315 ± 26 nm, the volume porosity was 94.5 ± 1.4%, the surface-to-volume ratio of the scaffolds was 25.4 ± 4.2 um−1 and the fiber surface roughness was 3.8 ± 0.6 nm. The scaffolds were characterized by a non-cytotoxicity effect and a high level of cytocompatibility with cells. The scaffolds also had high regenerative potential—the healing of the skin wound was accelerated by 19 days compared with the control. A histological analysis did not reveal any fragments of the experimental constructions or areas of inflammation. Thus, novel data on the structure and biological properties of the silk fibroin/spidroin electrospun scaffolds were obtained.

scholarly journals Pharmaceutical application of frog skin on full-thickness skin wound healing in mice

2013 ◽  
Vol 51 (12) ◽  
pp. 1600-1606 ◽  
Author(s):  
Mahere Rezazade Bazaz ◽  
Mohammad Mashreghi ◽  
Nasser Mahdavi Shahri ◽  
Mansour Mashreghi ◽  
Ahmad Asoodeh ◽  
...  
Keyword(s):  
Wound Healing ◽  
Frog Skin ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Pharmaceutical Application ◽  
Thickness Skin

Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model

Burns ◽  
2012 ◽  
Vol 38 (6) ◽  
pp. 820-829 ◽  
Author(s):  
Cécile Philandrianos ◽  
Lucile Andrac-Meyer ◽  
Serge Mordon ◽  
Jean-Marc Feuerstein ◽  
Florence Sabatier ◽  
...  
Keyword(s):  
Wound Healing ◽  
Porcine Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Dermal Substitutes ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

2022 ◽  
Vol 13 ◽  
pp. 204173142110630
Author(s):  
Peng Chang ◽  
Shijie Li ◽  
Qian Sun ◽  
Kai Guo ◽  
Heran Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Functional Unit ◽  
Collagen Gel ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
3D Printed

Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead “seed cells” as the bio-resource of engineering skin for large full-thickness skin wound healing.

Injectable Adhesive Self-Healing Multicross-Linked Double-Network Hydrogel Facilitates Full-Thickness Skin Wound Healing

2020 ◽  
Vol 12 (52) ◽  
pp. 57782-57797
Author(s):  
Bo Yang ◽  
Jiliang Song ◽  
Yuhang Jiang ◽  
Ming Li ◽  
Jingjing Wei ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Self Healing ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Double Network ◽  
Full Thickness Skin ◽  
Thickness Skin
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