Sperm Release From the Oviductal Epithelium Depends on Ca2+Influx Upon Activation of CB1 and TRPV1 by Anandamide

2015 ◽  
Vol 117 (2) ◽  
pp. 320-333 ◽  
Author(s):  
M.G. Gervasi ◽  
C. Osycka-Salut ◽  
T. Sanchez ◽  
C.A.I. Alonso ◽  
C. Llados ◽  
...  
Keyword(s):  
Sperm Release ◽  
Oviductal Epithelium

The endocannabinoid system in bull sperm and bovine oviductal epithelium: role of anandamide in sperm–oviduct interaction

Reproduction ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 403-414 ◽  
Author(s):  
María Gracia Gervasi ◽  
Maximiliano Rapanelli ◽  
María Laura Ribeiro ◽  
Mariana Farina ◽  
Silvia Billi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cannabinoid Receptors ◽  
Fatty Acid Amide Hydrolase ◽  
Acid Amide ◽  
Endocannabinoid System ◽  
Sperm Binding ◽  
Bovine Sperm ◽  
Sperm Release ◽  
Oviductal Epithelium ◽  
Bull Sperm

Anandamide binds to cannabinoid receptors and plays several central and peripheral functions. The aim of this work was to study the possible role for this endocannabinoid in controlling sperm–oviduct interaction in mammals. We observed that bull sperm and bovine oviductal epithelial cells express cannabinoid receptors, CB1 and CB2, and fatty acid amide hydrolase, the enzyme that controls intracellular anandamide levels. A quantitative assay to determine whether anandamide was involved in bovine sperm–oviduct interaction was developed. R(+)-methanandamide, a non-hydrolysable anandamide analog, inhibited sperm binding to and induced sperm release from oviductal epithelia. Selective CB1 antagonists (SR141716A or AM251) completely blocked R(+)-methanandamide effects. However, SR144528, a selective CB2 antagonist, did not exert any effect, indicating that only CB1 was involved in R(+)-methanandamide effect. This effect was not caused by inhibition of the sperm progressive motility or by induction of the acrosome reaction. Overall, our findings indicate for the first time that the endocannabinoid system is present in bovine sperm and oviductal epithelium and that anandamide modulates the sperm–oviduct interaction, by inhibition of sperm binding and induction of sperm release from oviductal epithelial cells, probably by activating CB1 receptors.

208 Sperm Retention, Storage and Release from the Oviduct: A Story of Sugars, Steroids, and Channels

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 113-114
Author(s):  
David J Miller
Keyword(s):  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
Sperm Protein ◽  
Cumulus Oocyte Complex ◽  
Sperm Release ◽  
Normal Increase ◽  
Complex Origin

Abstract Because mating is not always synchronized with ovulation, females from many species store sperm in the female reproductive tract until ovulation and fertilization. This may be done for short periods, a day or two for swine and cattle, or longer periods. Other mammals, such as some species of bats, store sperm for several months. Chickens and turkeys store sperm for 2–4 weeks and queens of some species of insects store sperm for over a decade in specialized structures. How sperm are retained, kept fertile for varying times and released is unclear. We have identified two specific carbohydrate motifs that are abundant in the porcine oviduct that bind and retain sperm in the isthmus. When immobilized, these two glycans lengthen sperm lifespan and suppress the normal increase in intracellular Ca2+ that normally accompanies capacitation. Porcine sperm can be released from oviduct cells and immobilized glycans by progesterone, perhaps of ovarian or cumulus-oocyte complex origin, which activates CatSper, a sperm-specific Ca2+ channel. Progesterone, as well as other compounds that stimulate hyperactivated motility, trigger sperm release, suggesting that hyperactivated motility is sufficient to release porcine sperm from oviduct glycans. We also have found that blocking proteasome-induced sperm protein lysis diminishes the number of sperm released from oviduct glycans. Finally, a transcriptomic approach has identified several groups of genes that are differentially regulated in both bovine and porcine oviducts from estrus animals that are storing sperm compared to oviducts from diestrus animals. This provides clues about how sperm lifespan is extended during storage.

Secretory activity in the oviductal epithelium of the stick insectCarausius morosus(Insecta, Phasmatodea)

1994 ◽  
Vol 61 (2) ◽  
pp. 105-113 ◽  
Author(s):  
Massimo Masetti ◽  
Ortenzio Fabiani ◽  
Franco Giorgi
Keyword(s):  
Secretory Activity ◽  
Oviductal Epithelium

scholarly journals Actin related protein complex subunit 1b controls sperm release, barrier integrity and cell division during adult rat spermatogenesis

2016 ◽  
Vol 1863 (8) ◽  
pp. 1996-2005 ◽  
Author(s):  
Anita Kumar ◽  
Kushaan Dumasia ◽  
Sharvari Deshpande ◽  
Reshma Gaonkar ◽  
N.H. Balasinor
Keyword(s):  
Cell Division ◽  
Protein Complex ◽  
Related Protein ◽  
Barrier Integrity ◽  
Adult Rat ◽  
Sperm Release ◽  
Rat Spermatogenesis

scholarly journals Reproductive biology of Palythoa caribaeorum and Protopalythoa variabilis (Cnidaria, Anthozoa, Zoanthidea) from the southeastern coast of Brazil

2005 ◽  
Vol 65 (1) ◽  
pp. 29-41 ◽  
Author(s):  
H. K. Boscolo ◽  
F. L. Silveira
Keyword(s):  
Reproductive Biology ◽  
High Frequency ◽  
Sao Paulo ◽  
São Paulo ◽  
Southeastern Coast ◽  
Female Sex ◽  
Sperm Release ◽  
Palythoa Caribaeorum ◽  
Maturation Stages

The reproductive biology of Palythoa caribaeorum (Duchassaing & Michelotti 1860) and Protopalythoa variabilis (Duerden 1898) was studied through monthly samples from tagged colonies from June 1996 to June 1997, in São Sebastião channel, São Paulo, Brazil (45º26'W, 23º50'S). The gametogenesis was similar to that of other zoanthids as shown by histological preparations. Oocyte diameters and maturation stages of testis vesicles were evaluated on squash preparations. Both species showed sequential protogynic hermaphroditism, with high frequency of fertile polyps (83% in P. variabilis and 72% in P. caribaeorum), high frequency of colonies in female sex condition (65.3% of P. variabilis and 41.7% of P. caribaeorum), and apparently continuous gametogenesis. In P. caribaeorum, egg release was continuous and sperm release took place during half of the analyzed period. In P. variabilis, egg and sperm release occurred in April-May and February-March 1997, respectively.

scholarly journals The Secretory Cells of the Goat Oviductal Epithelium: An Ultrastructural Study.

1995 ◽  
Vol 41 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Maki MORITA ◽  
Hajime MIYAMOTO ◽  
Takashi ISHII ◽  
Miki SUGIMOTO
Keyword(s):  
Ultrastructural Study ◽  
Secretory Cells ◽  
Oviductal Epithelium

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

scholarly journals Observations of Simultaneous Sperm Release and Larval Planulation Suggest Reproductive Assurance in the Coral Pocillopora acuta

2019 ◽  
Vol 6 ◽  
Author(s):  
Hillary A. Smith ◽  
Aurelie Moya ◽  
Neal E. Cantin ◽  
Madeleine J. H. van Oppen ◽  
Gergely Torda
Keyword(s):  
Reproductive Assurance ◽  
Sperm Release
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