The effect of luminal on growth, glucose utilization and oxygen consumption in mammalian cells in tissue culture

1958 ◽  
Vol 51 (3) ◽  
pp. 359-384 ◽  
Author(s):  
Samuel Dales ◽  
Kenneth C. Fisher
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Mammalian Cells ◽  
Glucose Utilization

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

scholarly journals SM1.4 - Lab-on-a-Chip System to Monitor the Oxygen Consumption of Mammalian Cells

2018 ◽  
Author(s):  
F. Bunge ◽  
S. van den Driesche ◽  
M. J. Vellekoop ◽  
A. Waite ◽  
U. Mirastschijski
Keyword(s):  
Oxygen Consumption ◽  
Mammalian Cells ◽  
Lab On A Chip

Metabolic Co-Operation Between Biochemically Marked Mammalian Cells in Tissue Culture

1969 ◽  
Vol 4 (2) ◽  
pp. 353-367
Author(s):  
H. SUBAK-SHARPE ◽  
R. R. BÜRK ◽  
J. D. PITTS
Keyword(s):  
Tissue Culture ◽  
Nucleic Acid ◽  
Mixed Culture ◽  
Molecular Basis ◽  
Mammalian Cells ◽  
Genetic Variant ◽  
Cell Contact ◽  
Metabolic Function ◽  
Indirect Contact ◽  
Fibroblast Line

Cells of a genetic variant of the hamster fibroblast line BHK 21 which lack inosinic pyrophosphorylase activity (IPP- cells) and therefore cannot normally incorporate [3H]hypoxanthine were grown in mixed culture with cells of BHK 21 sublines which have inosinic pyrophosphorylase activity (IPP+ cells). If not in contact with IPP+ cells, IPP- cells do not incorporate added [3H]hypoxanthine into nucleic acid. IPP+ cells always do incorporate [3H]hypoxanthine and IPP- cells when in direct or indirect contact with IPP+ cells also incorporate the isotope. Cell to cell contact appears to be essential for this gain of a metabolic function by IPP- cells. The possible molecular basis and general implications of the phenomenon are discussed.

scholarly journals Respiration and Glycolysis of Normal Human Lymphocytes

Blood ◽  
1966 ◽  
Vol 28 (2) ◽  
pp. 163-174 ◽  
Author(s):  
CARL J. HEDESKOV ◽  
VIGGO ESMANN
Keyword(s):  
Oxygen Consumption ◽  
Lactic Acid ◽  
Acid Production ◽  
Glucose Utilization ◽  
Aerobic Glycolysis ◽  
Human Lymphocytes ◽  
Lactic Acid Production ◽  
Crowding Effect ◽  
Normal Human

Abstract The metabolism of intact, normal, human lymphocytes in vitro was studied from a total of 80 subjects. Corrected for the metabolism of contaminating red blood cells, the glucose uptake, lactic acid production, and oxygen consumption were 62, 95, and 117 µmoles per 1010 lymphocytes per hour, respectively, provided the cells were incubated at concentrations greater than 40 x 106 lymphocytes per ml. At lower lymphocyte concentrations the oxygen consumption per lymphocyte rose steeply with decreasing cell concentration (crowding effect). A similar but weaker crowding effect was noted for the lactic acid production, but not for the utilization of glucose. The oxygen uptake was lower with 20 per cent than with 100 per cent oxygen as gas phase. Small Pasteur and Crabtree effects were demonstrated. The oxygen consumption and lactic acid production proceeded linear with time, while the glucose utilization was higher during the first 30 minutes of incubation than later on. It is concluded that lymphocytes have a low aerobic glycolysis accounting for 75 per cent of the glucose utilization. The respiration is severely inhibited at high cell concentrations and it is suggested that this is caused by an insufficient availability of oxygen to the cells.

EFFECTS OF ANAEROBIOSIS ON THE RATES OF MULTIPLICATION OF MAMMALIAN CELLS CULTURED IN VITRO

1960 ◽  
Vol 38 (1) ◽  
pp. 871-878 ◽  
Author(s):  
Samuel Dales
Keyword(s):  
Mammalian Cells ◽  
Glucose Utilization ◽  
Lactic Acid Production ◽  
Cell Types ◽  
In Vitro Cultures ◽  
Cell Multiplication ◽  
Embryonic Mouse ◽  
Mouse Cells ◽  
Oxidation Of Glucose

To test the effects of anaerobiosis on the rate of multiplication and carbohydrate metabolism of mammalian cells in vitro, cultures of a 'permanent' line, Earle's L strain cells, and of freshly explanted embryonic mouse cells were propagated in the presence and absence of oxygen. Contrary to the findings of several other investigators, our results show that the multiplication of both cell types was depressed by anaerobiosis. Anaerobiosis for at least 7 days, did not, however, bring about unbalanced growth in L cells, nor did it affect their capability to divide rapidly soon after they were returned to aerobic conditions. From the rates of glucose utilization, lactic acid production, and cell multiplication it was estimated that the rate of division in the two cell types studied was proportional to the energy which could be released from either glycolysis or complete oxidation of glucose.

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