The immunoglobulin-like cell adhesion molecule hepaCAM modulates cell adhesion and motility through direct interaction with the actin cytoskeleton

Vascular endothelial (VE)-cadherin is the endothelium-specific member of the cadherin family of homotypic cell adhesion molecules. VE-cadherin, but not the cell adhesion molecule platelet/endothelial cell adhesion molecule (PECAM-1), markedly colocalizes with actin stress fibers at cell-cell junctions between human umbilical vein endothelial cells. Inhibition of VE-cadherin-mediated, but not PECAM-1-mediated, adhesion induced reorganization of the actin cytoskeleton, loss of junctional VE-cadherin staining and loss of cell-cell adhesion. In functional assays, inhibition of VE-cadherin caused increased monolayer permeability and enhanced neutrophil transendothelial migration. In a complementary set of experiments, modulation of the actin cytoskeleton was found to strongly affect VE-cadherin distribution. Brief stimulation of the beta2-adrenergic receptor with isoproterenol induced a loss of actin stress fibers resulting in a linear, rather than ‘jagged’, VE-cadherin distribution. The concomitant, isoproterenol-induced, reduction in monolayer permeability was alleviated by a VE-cadherin-blocking antibody. Finally, cytoskeletal reorganization resulting from the inactivation of p21Rho caused a diffuse localization of VE-cadherin, which was accompanied by reduced cell-cell adhesion. Together, these data show that monolayer permeability and neutrophil transendothelial migration are modulated by VE-cadherin-mediated cell-cell adhesion, which is in turn controlled by the dynamics of the actin cytoskeleton.

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hepaCAM, a novel immunoglobulin‐like cell adhesion molecule, is associated with the actin cytoskeleton and the lipid rafts and is involved in cell‐matrix interaction

The FASEB Journal ◽

10.1096/fasebj.20.5.a1080-c ◽

2006 ◽

Vol 20 (5) ◽

Author(s):

Mei Chung Moh ◽

Shali Shen

Keyword(s):

Cell Adhesion ◽

Actin Cytoskeleton ◽

Lipid Rafts ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Matrix Interaction ◽

Cell Matrix

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A Direct Interaction of Axonin-1 with Ngcam-Related Cell Adhesion Molecule (Nrcam) Results in Guidance, but Not Growth of Commissural Axons

The Journal of Cell Biology ◽

10.1083/jcb.149.4.951 ◽

2000 ◽

Vol 149 (4) ◽

pp. 951-968 ◽

Cited By ~ 57

Author(s):

Dora Fitzli ◽

Esther T. Stoeckli ◽

Stefan Kunz ◽

Kingsley Siribour ◽

Christoph Rader ◽

...

Keyword(s):

Cell Adhesion ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Direct Interaction ◽

Longitudinal Axis ◽

Vitro Assay ◽

Commissural Axons ◽

Related Cell

An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti–axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.

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Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure

The Journal of Cell Biology ◽

10.1083/jcb.201009022 ◽

2011 ◽

Vol 192 (2) ◽

pp. 335-348 ◽

Cited By ~ 37

Author(s):

Caroline Laplante ◽

Laura A. Nilson

Keyword(s):

Drosophila Melanogaster ◽

Cell Adhesion ◽

Actin Cytoskeleton ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Leading Edge ◽

Scaffolding Protein ◽

Asymmetric Distribution ◽

Dorsal Closure ◽

Dorsal Midline

During Drosophila melanogaster dorsal closure, lateral sheets of embryonic epidermis assemble an actomyosin cable at their leading edge and migrate dorsally over the amnioserosa, converging at the dorsal midline. We show that disappearance of the homophilic cell adhesion molecule Echinoid (Ed) from the amnioserosa just before dorsal closure eliminates homophilic interactions with the adjacent dorsal-most epidermal (DME) cells, which comprise the leading edge. The resulting planar polarized distribution of Ed in the DME cells is essential for the localized accumulation of actin regulators and for actomyosin cable formation at the leading edge and for the polarized localization of the scaffolding protein Bazooka/PAR-3. DME cells with uniform Ed fail to assemble a cable and protrude dorsally, suggesting that the cable restricts dorsal migration. The planar polarized distribution of Ed in the DME cells thus provides a spatial cue that polarizes the DME cell actin cytoskeleton, defining the epidermal leading edge and establishing its contractile properties.

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Carcinoembryonic Antigen Cell Adhesion Molecule 1 Directly Associates with Cytoskeleton Proteins Actin and Tropomyosin

Journal of Biological Chemistry ◽

10.1074/jbc.m109110200 ◽

2001 ◽

Vol 276 (50) ◽

pp. 47421-47433 ◽

Cited By ~ 50

Author(s):

Detlef Schumann ◽

Charng-Jui Chen ◽

Bruce Kaplan ◽

John E. Shively

Keyword(s):

Cell Adhesion ◽

Fusion Protein ◽

Carcinoembryonic Antigen ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Binding Constants ◽

Direct Interaction ◽

Amino Acid Peptide ◽

Associated Proteins ◽

Cell Adhesion Molecule 1

CEA cell adhesion molecule 1 (CEACAM1), a type 1 transmembrane and homotypic cell adhesion protein belonging to the carcinoembryonic antigen (CEA) gene family and expressed on epithelial cells, is alternatively spliced to produce four major isoforms with three or four Ig-like ectodomains and either long (CEACAM1-L) or short (CEACAM1-S) cytoplasmic domains. When murine MC38 (methylcholanthrene-induced adenocarcinoma 38) cells were transfected with human CEACAM1-L and stimulated with sodium pervanadate, actin was found to co-localize with CEACAM1-L at cell-cell boundaries but not in untreated cells. When CEACAM1-L was immunoprecipitated from pervanadate-treated MC38/CEACAM1-L cells and the associated proteins were analyzed by two-dimensional gel analysis and mass spectrometry, actin and tropomyosin, among other proteins, were identified. Whereas a glutathioneS-transferase (GST) fusion protein containing thel-isoform (GST-Cyto-L) bound poorly to F-actin in a co-sedimentation assay, the S-isoform fusion protein (GST-Cyto-S) co-sedimented with F-actin, especially when incubated with G-actin during polymerization (KD= 7.0 μm). Both GST-Cyto-S and GST-Cyto-L fusion proteins bind G-actin and tropomyosin by surface plasmon resonance studies with binding constants of 0.7 × 10−8and 1.0 × 10−7mfor GST-Cyto-L to G-actin and tropomyosin, respectively, and 3.1 × 10−8and 1.3 × 10−7mfor GST-Cyto-S to G-actin and tropomyosin, respectively. Calmodulin or EDTA inhibited binding of the GST-Cyto-L fusion protein to G-actin, whereas calmodulin and G-actin, but not EDTA, stimulated binding to tropomyosin. A biotinylated 14-amino acid peptide derived from the juxtamembrane portion of the cytoplasmic domain of CEACAM1-L associated with both G-actin and tropomyosin withKDvalues of 1.3 × 10−5and 1.8 × 10−5m, respectively. These studies demonstrate the direct interaction of CEACAM1 isoforms with G-actin and tropomyosin and the direct interaction of CEACAM1-S with F-actin.

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Syntenin-1 and Ezrin Proteins Link Activated Leukocyte Cell Adhesion Molecule to the Actin Cytoskeleton

Journal of Biological Chemistry ◽

10.1074/jbc.m113.546754 ◽

2014 ◽

Vol 289 (19) ◽

pp. 13445-13460 ◽

Cited By ~ 24

Author(s):

Cicerone Tudor ◽

Joost te Riet ◽

Christina Eich ◽

Rolf Harkes ◽

Nick Smisdom ◽

...

Keyword(s):

Cell Adhesion ◽

Actin Cytoskeleton ◽

Adhesion Molecule ◽

Cell Adhesion Molecule

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Dynamic Regulation of Activated Leukocyte Cell Adhesion Molecule–mediated Homotypic Cell Adhesion through the Actin Cytoskeleton

Molecular Biology of the Cell ◽

10.1091/mbc.11.6.2057 ◽

2000 ◽

Vol 11 (6) ◽

pp. 2057-2068 ◽

Cited By ~ 55

Author(s):

Judith M. D. T. Nelissen ◽

Inge M. Peters ◽

Bart G. de Grooth ◽

Yvette van Kooyk ◽

Carl G. Figdor

Keyword(s):

Cell Adhesion ◽

Actin Cytoskeleton ◽

Adhesion Molecule ◽

Actin Polymerization ◽

Cell Adhesion Molecule ◽

Cluster Formation ◽

Single Particle Tracking ◽

Lateral Mobility ◽

Surface Distribution ◽

Dynamic Regulation

Restricted expression of activated leukocyte cell adhesion molecule (ALCAM) by hematopoietic cells suggests an important role in the immune system and hematopoiesis. To get insight into the mechanisms that control ALCAM-mediated adhesion we have investigated homotypic ALCAM–ALCAM interactions. Here, we demonstrate that the cytoskeleton regulates ALCAM-mediated cell adhesion because inhibition of actin polymerization by cytochalasin D (CytD) strongly induces homotypic ALCAM–ALCAM interactions. This induction of cell adhesion is likely due to clustering of ALCAM at the cell surface, which is observed after CytD treatment. Single-particle tracking demonstrated that the lateral mobility of ALCAM in the cell membrane is increased 30-fold after CytD treatment. In contrast, both surface distribution and adhesion of a glycosylphosphatidylinositol (GPI)-anchored ALCAM mutant are insensitive to CytD, despite the increase in lateral mobility of GPI-ALCAM upon CytD treatment. This demonstrates that clustering of ALCAM is essential for cell adhesion, whereas enhanced diffusion of ALCAM alone is not sufficient for cluster formation. In addition, upon ligand binding, both free diffusion and the freely dragged distance of wild-type ALCAM, but not of GPI-ALCAM, are reduced over time, suggesting strengthening of the cytoskeleton linkage. From these findings we conclude that activation of ALCAM-mediated adhesion is dynamically regulated through actin cytoskeleton-dependent clustering.

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