scholarly journals CD93 hematopoietic stem cells improve diabetic wound healing by VEGF activation and downregulation of DAPK‐1

2019 ◽  
Vol 235 (3) ◽  
pp. 2366-2376 ◽  
Author(s):  
Fariba Zafari ◽  
Sadegh Shirian ◽  
Morteza Sadeghi ◽  
Shahram Teimourian ◽  
Mehrdad Bakhtiyari
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Hematopoietic Stem Cells ◽  
Diabetic Wound ◽  
Hematopoietic Stem ◽  
Diabetic Wound Healing

Mobilization of autologous hematopoietic stem cells by targeting the CXCR4-SDF-1a axis to modulate diabetic wound healing

2007 ◽  
Vol 205 (3) ◽  
pp. S66
Author(s):  
Giorgio Pietramaggiori ◽  
Paolo Fiorina ◽  
Saja Scherer ◽  
Jasimine Mathews ◽  
Mollie Jurewicz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Hematopoietic Stem Cells ◽  
Diabetic Wound ◽  
Hematopoietic Stem ◽  
Diabetic Wound Healing

Extracellular vesicles derived from adipose-derived stem cells accelerate diabetic wound healing by suppressing the expression of matrix metalloproteinase-9

2021 ◽  
Vol 22 ◽  
Author(s):  
Jiang-wen Wang ◽  
Yuan-zheng Zhu ◽  
Xuan Hu ◽  
Jia-ying Nie ◽  
Zhao-hui Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mouse Model ◽  
Adipose Derived Stem Cells ◽  
Collagen Deposition ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds ◽  
Metalloproteinase 9

Background: The healing of diabetic wounds is poor due to a collagen deposition disorder. Matrix metalloproteinase-9 (MMP-9) is closely related to collagen deposition in the process of tissue repair. Many studies have demonstrated that extracellular vesicles derived from adipose-derived stem cells (ADSC-EVs) promote diabetic wound healing by enhancing collagen deposition. Objective: In this study, we explored if ADSC-EVs could downregulate the expression of MMP-9 in diabetic wounds and promote wound healing by improving collagen deposition. The potential effects of ADSC-EVs on MMP-9 and diabetic wound healing were tested both in vitro and in vivo. Methods: We first evaluated the effect of ADSC-EVs on the proliferation and MMP-9 secretion of HaCaT cells treated with advanced glycation end product-bovine serum albumin (AGE-BSA), using CCK-8 western blot and MMP-9 enzyme-linked immunosorbent assay(ELISA). Next, the effect of ADSC-EVs on the healing, re-epithelialisation, collagen deposition, and MMP-9 concentration in diabetic wound fluids was evaluated in an immunodeficient mouse model via MMP-9 ELISA and haematoxylin and eosin, Masson’s trichrome, and immunofluorescence staining for MMP-9. Results: In vitro, ADSC-EVs promoted the proliferation and MMP-9 secretion of HaCaT cells.In vivo, ADSC-EVs accelerated diabetic wound healing by improving re-epithelialisation and collagen deposition and by inhibiting the expression of MMP-9. Conclusion: ADSC-EVs possessed the healing of diabetic wounds in a mouse model by inhibiting downregulating MMP-9 and improving collagen deposition.Thus ,ADSC-EVs are a promising candidate for the treatment of diabetic wounds .

Human mesenchymal stem cells-conditioned medium improves diabetic wound healing mainly through modulating fibroblast behaviors

2019 ◽  
Vol 312 (5) ◽  
pp. 325-336 ◽  
Author(s):  
Mona Saheli ◽  
Mohammad Bayat ◽  
Rasoul Ganji ◽  
Farzane Hendudari ◽  
Raziyeh Kheirjou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Human Mesenchymal Stem Cells ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

Adipose-derived stem cells: Effectiveness and advances in delivery in diabetic wound healing

2018 ◽  
Vol 107 ◽  
pp. 625-633 ◽  
Author(s):  
Mohamed Gadelkarim ◽  
Abdelrahman Ibrahim Abushouk ◽  
Esraa Ghanem ◽  
Ali Mohamed Hamaad ◽  
Anas M. Saad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

scholarly journals Enhanced Healing of Diabetic Wounds by Subcutaneous Administration of Human Umbilical Cord Derived Stem Cells and Their Conditioned Media

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Chandrama Shrestha ◽  
Liling Zhao ◽  
Ke Chen ◽  
Honghui He ◽  
Zhaohui Mo
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Umbilical Cord ◽  
Subcutaneous Administration ◽  
Conditioned Media ◽  
Human Umbilical Cord ◽  
Diabetic Wound ◽  
Significant Difference ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds

Objective. Mesenchymal stem cells (MSCs) isolated from the umbilical cord and their conditioned media (CM) can be easily obtained and refined compared with stem cells from other sources. Here, we explore the possibility of the benefits of these cells in healing diabetic wounds.Methodology and Results. Delayed wound healing animal models were established by making a standard wound on the dorsum of eighteen db/db mice, which were divided into three groups with six mice in each: groups I, II, and III received PBS, UC-MSC, and CM, respectively. UC-MSC and their CM significantly accelerated wound closure compared to PBS-treated wounds, and it was most rapid in CM-injected wounds. In day-14 wounds, significant difference in capillary densities among the three groups was noted (n=6;P<0.05), and higher levels of VEGF, PDGF, and KGF expression in the CM- and UC-MSC-injected wounds compared to the PBS-treated wounds were seen. The expression levels of PDGF-βand KGF were higher in CM-treated wounds than those in UC-MSC-treated wounds.Conclusion. Both the transplantation of UC-MSC and their CM are beneficial to diabetic wound healing, and CM has been shown to be therapeutically better than UC-MSC, at least in the context of diabetic wound healing.

Accelerated diabetic wound healing in a murine model with the application of multipotent human adipose derived stem cells

2006 ◽  
Vol 203 (3) ◽  
pp. S43 ◽  
Author(s):  
Anna M. Parker ◽  
George Rodeheaver ◽  
Lisa Salopek ◽  
Hulan Shang ◽  
Moshe Khurgel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Murine Model ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

scholarly journals Mesenchymal stem cells correct impaired diabetic wound healing by decreasing ECM proteolysis

2017 ◽  
Vol 49 (10) ◽  
pp. 541-548 ◽  
Author(s):  
Junwang Xu ◽  
Carlos Zgheib ◽  
Maggie M. Hodges ◽  
Robert C. Caskey ◽  
Junyi Hu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Protein Content ◽  
Collagen I ◽  
Extracellular Matrix Protein ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds

Impaired diabetic wound healing is associated with a dermal extracellular matrix protein profile favoring proteolysis; within the healing diabetic wound, this is represented by an increase in activated matrix metalloproteinase (MMPs). Treatment of diabetic wounds with mesenchymal stem cells (MSCs) has been shown to improve wound healing; however, there has not yet been an assessment of their ability to correct dysregulation of MMPs in diabetic wounds. Furthermore, there has been no prior assessment of the role of microRNA29b (miR-29b), an inhibitory regulatory molecule that targets MMP-9 mRNA. Using in vitro models of fibroblast coculture with MSCs and in vivo murine wound healing models, we tested the hypothesis that MSCs correct dysregulation of MMPs in a microRNA-29b-dependent mechanism. In this study, we first demonstrated that collagen I and III protein content is significantly reduced in diabetic wounds, and treatment with MSCs significantly improves collagen I content in both nondiabetic and diabetic wounds. We then found that MMP-9 gene expression and protein content were significantly upregulated in diabetic wounds, indicating elevated proteolysis. Treatment with MSCs resulted in a decrease in MMP-9 gene expression and protein content level in diabetic wounds 3 and 7 days after wounding. Zymographic analysis indicated that MSC treatment also decreased the amount of activated MMP-9 present in diabetic wounds. Furthermore, miR-29b expression was inversely associated with MMP-9 gene expression; miR-29b expression was decreased in diabetic wounds and diabetic fibroblast. Following treatment of diabetic wounds with MSCs, as well as in diabetic fibroblasts cocultured with MSCs, miR-29b was significantly increased. These findings suggest a potential mechanism through which MSCs enhance diabetic wound healing by improving collagen I content in diabetic wounds through decreasing MMP-9 expression and increasing miR-29b expression.

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