Enhanced nitrogen removal and in situ microbial community in a two-step feed oxic/anoxic/oxic-membrane bioreactor (O/A/O-MBR) process

2019 ◽  
Vol 94 (4) ◽  
pp. 1315-1322 ◽  
Author(s):  
Ning Li ◽  
Wei Zeng ◽  
Zhijia Miao ◽  
Baogui Wang ◽  
Zaixing Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Membrane Bioreactor

scholarly journals Variations in microbial community during nitrogen removal by in situ oxygen-enhanced indigenous nitrogen-removal bacteria

2018 ◽  
Vol 11 (4) ◽  
pp. 276-287 ◽  
Author(s):  
Shi-lei Zhou ◽  
Yue Sun ◽  
Yi-ran Zhang ◽  
Ting-lin Huang ◽  
Zai-xing Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal

scholarly journals Performance and microbial community of the CANON process in a sequencing batch membrane bioreactor with elevated COD/N ratios

2020 ◽  
Vol 81 (1) ◽  
pp. 138-147
Author(s):  
Xiaoling Zhang ◽  
Xincong Liu ◽  
Meng Zhang
Keyword(s):  
Organic Matter ◽  
Microbial Community ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Ammonia Oxidation ◽  
Heterotrophic Bacteria ◽  
Removal Rate ◽  
Ammonia Oxidizing Bacteria ◽  
Anaerobic Ammonia Oxidation ◽  
Canon Process

Abstract In this study, the effects of elevated chemical oxygen demand/nitrogen (COD/N) ratios on nitrogen removal, production and composition of the extracellular polymer substances (EPS) and microbial community of a completely autotrophic nitrogen removal via nitrite (CANON) process were studied in a sequencing batch membrane bioreactor (SBMBR). The whole experiment was divided into two stages: the CANON stage (without organic matter in influent) and the simultaneous partial nitrification, anaerobic ammonia oxidation and denitrification (SNAD) stage (with organic matter in influent). When the inflow ammonia nitrogen was 420 mg/L and the COD/N ratio was no higher than 0.8, the addition of COD was helpful to the CANON process; the total nitrogen removal efficiency (TNE) was improved from approximately 65% to more than 75%, and the nitrogen removal rate (NRR) was improved from approximately 0.255 kgN/(m3·d) to approximately 0.278 kgN/(m3•d), while the TNE decreased to 60%, and the NRR decreased to 0.236 kgN/(m3•d) when the COD/N ratio was elevated to 1.0. For the EPS, the amounts of soluble EPS (SEPS) and loosely bound EPS (LB-EPS) were both higher in the CANON stage than in the SNAD stage, while the amount of tightly bound EPS (TB-EPS) in the SNAD stage was significantly higher due to the proliferation of heterotrophic bacteria. The metagenome sequencing technique was used to analyse the microbial community in the SBMBR. The results showed that the addition of COD altered the structure of the bacterial community in the SBMBR. The amounts of Candidatus ‘Anammoxoglobus’ of anaerobic ammonia oxidation bacteria (AAOB) and Nitrosomonas of ammonia oxidizing bacteria (AOB) both decreased significantly, and Nitrospira of nitrite oxidizing bacteria (NOB) was always in the reactor, although the amount changed slightly. A proliferation of denitrifiers related to the genera of Thauera, Dokdonella and Azospira was found in the SBMBR.

The Research of Fluorescence In Situ Hybridization Approach Applied in Denitrification Bacteria in Metal Membrane Bioreactor

2013 ◽  
Vol 864-867 ◽  
pp. 1490-1493
Author(s):  
Pan Zhang ◽  
Yuan Hua Xie
Keyword(s):  
Population Structure ◽  
In Situ Hybridization ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Metal Membrane ◽  
Quality Of Water ◽  
Preliminary Study ◽  
Optimized Conditions

To research the composition and proportion of the denitrying bacterium in denitrification tank and increase the nitrogen removals rate. With the purpose of explore the curve between the amount of different denitrying bacterium and the quality of water treating, then find out the optimum of the species of nitrogen removal bacteria and the amount of the specific type of microorganism in the denitrification tank of metal membrane bioreactor at the optimized conditions of the water treating effects. The fluorescence in situ hybridization (FISH) approach is applied in this research, to set a preliminary study on the space-time character of denitrification bacteria population structure in denitrifical tank.

Bioelectrochemically-assisted nitrogen removal in osmotic membrane bioreactor

2020 ◽  
Author(s):  
You Wu ◽  
Yun Cai ◽  
Yu-Xiang Lu ◽  
Li-Min Zhang ◽  
Xiao-Li Yang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Operation Mode ◽  
Oxygen Demand ◽  
Denitrifying Bacteria ◽  
Future Research ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrite Oxidizing Bacteria

Abstract Nitrogen removal in osmosis membrane bioreactor (OMBR) is important to its applications but remains a challenge. In this study, a bioelectrochemically-assisted (BEA) operation was integrated into the feed side of OMBRs to enhance nitrogen removal, and sodium acetate was served as a draw solute and supplementary carbon source for the growth of denitrifying bacteria due to reversed-solute. The effects of operation mode and influent ammonium (NH4+) concentration were systematically examined. Compared to a conventional OMBR, the integrated BEA-OMBR achieved higher total nitrogen removal efficiency of 98.13%, and chemical oxygen demand removal efficiency of 95.83% with the influent NH4+-N concentration of 39 mg L−1. The sequencing analyses revealed that ammonia-oxidizing bacteria (0–0.04%), nitrite-oxidizing bacteria (0–0.16%), and denitrifying bacteria (1.98–8.65%) were in abundance of the microbial community in the feed/anode side of integrated BEA-OMBR, and thus BEA operation increased the diversity of the microbial community in OMBR. Future research will focus on improving nitrogen removal from a high ammonium strength wastewater by looping anolyte effluent to the cathode. These findings have demonstrated that BEA operation can be an effective approach to improve nitrogen removal in OMBRs toward sustainable wastewater treatment.

In-situ characterization of microbial community in an A/O submerged membrane bioreactor with nitrogen removal

2004 ◽  
Vol 50 (8) ◽  
pp. 41-48 ◽  
Author(s):  
A. Sofia ◽  
W.-T. Liu ◽  
S.L. Ong ◽  
W.J. Ng
Keyword(s):  
Bacterial Community ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Ammonia Oxidation ◽  
Paracoccus Denitrificans ◽  
Pseudomonas Stutzeri ◽  
Laboratory Scale ◽  
Negative Results

The bacterial community involved in removing nitrogen from sewage and their preferred DO environment within an anoxic/oxic membrane bioreactor (A/O MBR) was investigated. A continuously operated laboratory-scale A/O MBR was maintained for 360 d. At a sludge age of 150 d and a C/N ratio of 3.5, the system was capable of removing 88% of the influent nitrogen from raw wastewater through typical nitrogen removal transformations (i.e. aerobic ammonia oxidation and anoxic nitrate reduction). Characterization of the A/O MBR bacterial community was carried out using fluorescence in situ hybridization (FISH) techniques. FISH results further showed that Nitrosospira spp. and Nitrospira spp. were the predominant groups of ammonia and nitrite oxidizing group, respectively. They constituted up to 11% and 6% of eubacteria at DO below 0.05 mg/l (low DO), respectively, and about 14% and 9% of eubacteria at DO between 2–5 mg/l (sufficient DO), respectively, indicating preference of nitrifiers for a higher DO environment. Generally low counts of the genus Paracoccus were detected while negative results were observed for Paracoccus denitrificans, Alcaligenes spp, and Pseudomonas stutzeri under the low and sufficient DO environments. The overall results indicate that Nitrosospira spp., Nitrospira spp. and members of Paracoccus spp. can be metabolically functional in nitrogen removal in the laboratory-scale A/O MBR system.

Sign in / Sign up

Export Citation Format

Share Document