The Research of Fluorescence In Situ Hybridization Approach Applied in Denitrification Bacteria in Metal Membrane Bioreactor

2013 ◽  
Vol 864-867 ◽  
pp. 1490-1493
Author(s):  
Pan Zhang ◽  
Yuan Hua Xie
Keyword(s):  
Population Structure ◽  
In Situ Hybridization ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Metal Membrane ◽  
Quality Of Water ◽  
Preliminary Study ◽  
Optimized Conditions

To research the composition and proportion of the denitrying bacterium in denitrification tank and increase the nitrogen removals rate. With the purpose of explore the curve between the amount of different denitrying bacterium and the quality of water treating, then find out the optimum of the species of nitrogen removal bacteria and the amount of the specific type of microorganism in the denitrification tank of metal membrane bioreactor at the optimized conditions of the water treating effects. The fluorescence in situ hybridization (FISH) approach is applied in this research, to set a preliminary study on the space-time character of denitrification bacteria population structure in denitrifical tank.

The Fluorescence In Situ Hybridization Approach Applied in the Study of the Denitrifying Bacteria on Nitration Tank of the Metal Membrane Bioreactor

2011 ◽  
Vol 291-294 ◽  
pp. 2686-2689
Author(s):  
Pan Zhang ◽  
Lei Chen
Keyword(s):  
Membrane Bioreactor ◽  
Denitrifying Bacteria ◽  
Ammonia Nitrogen ◽  
Space Distribution ◽  
Nitrifying Bacteria ◽  
Active Sludge ◽  
Metal Membrane ◽  
The Relationship

To study the amount changes of different denitrifying bacteria in the process of the metal membrane bioreactor, and to confirm the relationship between the amount of different bacteria and the quality of the treating water, the fluorescence in situ hybridization (FISH) dominant molecular biological technologies were applied in this study to preliminary explore the time and space distribution of different denitrifying bacteria populations in the active sludge on the immersed plate-type metal membrane bioreactor. The result indicates that the involvement of the denitrification tank is beneficial to the growth of all bacteria and nitrifying bacteria, meanwhile the eliminate rate of the total nitrogen and ammonia nitrogen is also improved.

scholarly journals Quantitative Single-Cell Transcript Assessment of Biomarkers Supports Cellular Heterogeneity in the Bovine IVD

2019 ◽  
Vol 6 (2) ◽  
pp. 42 ◽  
Author(s):  
Kangning Li ◽  
Devin Kapper ◽  
Sumona Mondal ◽  
Thomas Lufkin ◽  
Petra Kraus
Keyword(s):  
In Situ Hybridization ◽  
Single Cell ◽  
Gaussian Mixture ◽  
Cellular Heterogeneity ◽  
Rna In Situ Hybridization ◽  
Ivd Degeneration

Severe and chronic low back pain is often associated with intervertebral disc (IVD) degeneration. While imposing a considerable socio-economic burden worldwide, IVD degeneration is also severely impacting on the quality of life of affected individuals. Cell-based regenerative medicine approaches have moved into clinical trials, yet IVD cell identities in the mature disc remain to be fully elucidated and tissue heterogeneity exists, requiring a better characterization of IVD cells. The bovine coccygeal IVD is an accepted research model to study IVD mechano-biology and disc homeostasis. Recently, we identified novel IVD biomarkers in the outer annulus fibrosus (AF) and nucleus pulposus (NP) of the mature bovine coccygeal IVD through RNA in situ hybridization (AP-RISH) and z-proportion test. Here we follow up on Lam1, Thy1, Gli1, Gli3, Noto, Ptprc, Scx, Sox2 and Zscan10 with fluorescent RNA in situ hybridization (FL-RISH) and confocal microscopy. This permits sub-cellular transcript localization and the addition of quantitative single-cell derived values of mRNA expression levels to our previous analysis. Lastly, we used a Gaussian mixture modeling approach for the exploratory analysis of IVD cells. This work complements our earlier cell population proportion-based study, confirms the previously proposed biomarkers and indicates even further heterogeneity of cells in the outer AF and NP of a mature IVD.

Seismic Vulnerability of Buried Water Pipes

2012 ◽  
Vol 518-523 ◽  
pp. 3740-3743 ◽  
Author(s):  
Halfaya Fatma Zohra ◽  
Mahmoud Bensaibi ◽  
Davenne Luc
Keyword(s):  
Seismic Vulnerability ◽  
Large Degree ◽  
Vulnerability Index ◽  
Water Network ◽  
Buried Pipelines ◽  
Sanitation Services ◽  
Quality Of Water ◽  
Good Classification

In south hemisphere regions mortality rates are closely related to infectious diseases that, to a large degree, depend on the quality of water consumed and on access to adequate sanitation services. A special attention must be paid to water quality particularly in case of an earthquake. Damage in this sector depends not only on the intensity of the disaster, but also on the vulnerability of buried pipelines. In this work, this vulnerability is studied through the use of a developed vulnerability index. This one allows a good classification and description of the seismic quality of the pipes taking into account the main parameters governing their vulnerability. A program including all informations that might be used was subsequently developed. The method was applied on the water network of Blida, a city in suburb of Algiers. The results obtained show that the established classification confirms the observed information in situ.

scholarly journals UroVysion Fluorescence in situ hybridization (UroVysion FISH) assay for detecting Turkish bladder cancer patients in voided urine: A preliminary study

2013 ◽  
Vol 2 ◽  
pp. 156-160
Author(s):  
Yavuz Dodurga ◽  
ÇIğır Biray Avcı ◽  
Sunde Yılmaz ◽  
Oktay NazIı ◽  
Özgür Çoğulu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cancer Patients ◽  
Preliminary Study

Lessons learnt from the application of a multi-variable controller for nitrogen removal in the Mekolalde wastewater treatment plant: good simulation practices in control

2014 ◽  
Vol 69 (6) ◽  
pp. 1289-1297 ◽  
Author(s):  
I. Irizar ◽  
S. Beltrán ◽  
G. Urchegui ◽  
G. Izko ◽  
O. Fernández ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Real Life ◽  
Control Product ◽  
Quality Of Water ◽  
Short Period ◽  
Mathematical Modelling And Simulation

Although often perceived as tools for use by scientists, mathematical modelling and simulation become indispensable when control engineers have to design controllers for real-life wastewater treatment plants (WWTPs). Nonetheless, the design of effective controllers in the wastewater domain using simulations requires effects, such as the nonlinearity of actuators, the time response of sensors, plant model uncertainties, etc. to have been reproduced beforehand. Otherwise, control solutions verified by simulation can completely underperform under real conditions. This study demonstrates that, when all the above effects are included at the outset, a systematic use of simulations guarantees high quality controllers in a relatively short period of time. The above is exemplified through the Mekolalde WWTP, where a comprehensive simulation study was conducted in order to develop a control product for nitrogen removal. Since its activation in May 2011, the designed controller has been permanently working in the plant which, from this time onwards, has experienced significant improvements in the quality of water discharges combined with a lower utilization of electricity for wastewater treatment.

Enhanced nitrogen removal and in situ microbial community in a two-step feed oxic/anoxic/oxic-membrane bioreactor (O/A/O-MBR) process

2019 ◽  
Vol 94 (4) ◽  
pp. 1315-1322 ◽  
Author(s):  
Ning Li ◽  
Wei Zeng ◽  
Zhijia Miao ◽  
Baogui Wang ◽  
Zaixing Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Membrane Bioreactor

SUITABILITY OF LOW TEMPERATURE VACUUM PAN EVAPORATOR CONDENSATE FOR PLANT USES

1966 ◽  
Vol 29 (2) ◽  
pp. 40-44
Author(s):  
Daniel A. Stock
Keyword(s):  
Low Temperature ◽  
Skim Milk ◽  
Water Source ◽  
Additional Treatment ◽  
Pan Evaporation ◽  
Quality Of Water ◽  
Plant Uses ◽  
Preliminary Study ◽  
Exchange Medium

Summary A preliminary study to investigate the possibility of utilizing condensates and tailwater from the low temperature vacuum pan evaporation of skim milk for evaporating plant uses was made. The results indicated that the utilization of these condensates and tailwaters for various plant purposes is possible and should provide a readily available, safe and sanitary water source if adequate steps such as quality monitoring and treatment are taken to insure that the highest quality of water is retained and used. The use of tailwater as a heat exchange medium on a single pass basis should require only quality control monitoring. However, condensate or tailwater which is to be used for other purposes should be aerated and may need additional treatment to prevent the development of tastes, odors, growths, corrosion, and scale formation.

The Study of the Fluorescence In Situ Hybridization Applied in the Denitrifying Bacteria on Metal Membrane Bioreactor’s Denitrification Tank

2011 ◽  
Vol 317-319 ◽  
pp. 250-253
Author(s):  
Pan Zhang ◽  
Lei Chen
Keyword(s):  
Membrane Bioreactor ◽  
Removal Rate ◽  
Denitrifying Bacteria ◽  
Ammonia Nitrogen ◽  
Nitrifying Bacteria ◽  
Active Sludge ◽  
Metal Membrane ◽  
The Relationship ◽  
Plate Type

In order to study changes in the quantity of different denitrifying bacteria during the process of the metal membrane bioreactor, and to determine the relationship between different types of bacteria’s quantity and the treating water quality, the fluorescence in situ hybridization (FISH) dominant molecular biological technologies were used in this study to explore different denitrifying bacteria populations in the active sludge in the denitrification tank on the immersed plate-type metal membrane bioreactor. The study result implies that the addition involvement of the denitrification tank is enormous beneficial to the growth of all bacteria and nitrifying bacteria, meanwhile the removal rate of total nitrogen and ammonia nitrogen is improved as well.

scholarly journals Status quo of ALK testing in lung cancer: results of an EQA scheme based on in-situ hybridization, immunohistochemistry, and RNA/DNA sequencing

2021 ◽  
Author(s):  
Philipp Jurmeister ◽  
Claudia Vollbrecht ◽  
Korinna Jöhrens ◽  
Daniela Aust ◽  
Anke Behnke ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Dna Sequencing ◽  
Online Questionnaire ◽  
Tissue Quality ◽  
Ring Trial ◽  
Alk Positive ◽  
Technical Capacity

AbstractWith this external quality assessment (EQA) scheme, we aim to investigate the diagnostic performance of the currently available methods for the detection of ALK alterations in non-small cell lung cancer on a national scale, namely, in situ hybridization (ISH), immunohistochemistry (IHC), and RNA/DNA sequencing (NGS). The EQA scheme cohort consisted of ten specimens, including four ALK positive and six ALK negative samples, which were thoroughly pretested using IHC, ISH, and RNA/DNA NGS. Unstained tumor sections were provided to the 57 participants, and the results were retrieved via an online questionnaire. ISH was used by 29, IHC by 38, and RNA/DNA sequencing by 19 participants. Twenty-eight institutions (97%) passed the ring trial using ISH, 33 (87%) by using IHC, and 18 (95%) by using NGS. The highest sensitivity and interrater agreement (Fleiss ‘ kappa) was observed for RNA/DNA sequencing (99%, 0.975), followed by ISH (94%, 0.898) and IHC (92%, 0.888). However, the proportion of samples that were not evaluable due to bad tissue quality was also higher for RNA/DNA sequencing (4%) compared with ISH (0.7%) and IHC (0.5%). While all three methods produced reliable results between the different institutions, the highest sensitivity and concordance were observed for RNA/DNA sequencing. These findings encourage the broad implementation of this method in routine diagnostic, although the application might be limited by technical capacity, economical restrictions, and tissue quality of formalin-fixed samples.

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