Analysis of bacterial communities in pit mud from Zhijiang Baijiu distillery using denaturing gradient gel electrophoresis and high- throughput sequencing

2019 ◽  
Vol 126 (1) ◽  
pp. 90-97 ◽  
Author(s):  
Guangxun Tan ◽  
Yuanliang Hu ◽  
Yinna Huang ◽  
Huanming Liu ◽  
Weiwei Dong ◽  
...  
Keyword(s):  
High Throughput ◽  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
High Throughput Sequencing ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Pit Mud ◽  
Gradient Gel Electrophoresis

Characterization of the Microbiota in Air- or Vacuum-Packed Crisp Grass Carp (Ctenopharyngodon idella C. et V.) Fillets by 16S rRNA PCR–Denaturing Gradient Gel Electrophoresis and High-Throughput Sequencing

2018 ◽  
Vol 81 (6) ◽  
pp. 1022-1029 ◽  
Author(s):  
ZIQIANG PAN ◽  
LIN LI ◽  
ZHIHUA SHEN ◽  
YAN CHEN ◽  
MEI LI
Keyword(s):  
16S Rrna ◽  
High Throughput ◽  
Grass Carp ◽  
Gel Electrophoresis ◽  
High Throughput Sequencing ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Ctenopharyngodon Idella ◽  
Chilled Storage ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Gradient Gel Electrophoresis

ABSTRACT The microbial communities in air- and vacuum-packed crisp grass carp (Ctenopharyngodon idella C. et V.) fillets have not been characterized during chilled storage. High-throughput sequencing of bacterial 16S rRNA has now revealed that the bacterial community in fresh fillets is diverse and distinct from that in spoiled samples. The predominant phylum was Proteobacteria, and 66 genera were identified. In fresh fillets, the most abundant genera were Acinetobacter (53.3%), Wautersiella (6.3%), unclassified Alcaligenaceae (4.4%), Stenotrophomonas (3.8%), unclassified Enterobacteriaceae (3.8%), and Enhydrobacter (3.6%). These genera diminished during chilled storage and sometimes disappeared. At the end of storage, Aeromonas and Pseudomonas were the most abundant. Similar results were obtained by PCR–denaturing gradient gel electrophoresis. These data provide detailed insight into the evolving bacterial communities in air- and vacuum-packed crisp grass carp fillets during storage, revealing Aeromonas and Pseudomonas as major spoilage organisms. These data may be useful for improvement of crisp grass carp quality and shelf life during chilled storage.

scholarly journals Impact of Intensive Land-Based Fish Culture in Qingdao, China, on the Bacterial Communities in Surrounding Marine Waters and Sediments

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Qiufen Li ◽  
Yan Zhang ◽  
David Juck ◽  
Nathalie Fortin ◽  
Charles W. Greer
Keyword(s):  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Fish Culture ◽  
Fish Ponds ◽  
Marine Area ◽  
Gradient Gel Electrophoresis ◽  
Reducing Bacteria ◽  
The Impact ◽  
Nitrate Reducing Bacteria

The impact of intensive land-based fish culture in Qingdao, China, on the bacterial communities in surrounding marine environment was analyzed. Culture-based studies showed that the highest counts of heterotrophic, ammonium-oxidizing, nitrifying, and nitrate-reducing bacteria were found in fish ponds and the effluent channel, with lower counts in the adjacent marine area and the lowest counts in the samples taken from 500 m off the effluent channel. Denaturing gradient gel electrophoresis (DGGE) analysis was used to assess total bacterial diversity. Fewer bands were observed from the samples taken from near the effluent channel compared with more distant sediment samples, suggesting that excess nutrients from the aquaculture facility may be reducing the diversity of bacterial communities in nearby sediments. Phylogenetic analysis of the sequenced DGGE bands indicated that the bacteria community of fish-culture-associated environments was mainly composed of Flavobacteriaceae, gamma- and deltaproteobacteria, including generaGelidibacter, Psychroserpen, Lacinutrix,andCroceimarina.

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