Mass spectrometry combined with affinity probes for the identification of CP4 EPSPS in genetically modified soybeans

2019 ◽  
Vol 55 (2) ◽  
pp. e4371
Author(s):  
Shobha Devi ◽  
Pei-Yu Chu ◽  
Bo-Hung Wu ◽  
Yen-Peng Ho
Keyword(s):  
Mass Spectrometry ◽  
Genetically Modified ◽  
Affinity Probes ◽  
Genetically Modified Soybeans ◽  
Cp4 Epsps

scholarly journals Serum testing of genetically modified soybeans with special emphasis on potential allergenicity of the heterologous protein CP4 EPSPS

2007 ◽  
Vol 51 (8) ◽  
pp. 946-955 ◽  
Author(s):  
Michael Hoff ◽  
Dae-Yeul Son ◽  
Michaela Gubesch ◽  
Kangmo Ahn ◽  
Sang-Il Lee ◽  
...  
Keyword(s):  
Heterologous Protein ◽  
Genetically Modified ◽  
Serum Testing ◽  
Genetically Modified Soybeans ◽  
Cp4 Epsps

scholarly journals Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 522 ◽  
Author(s):  
Ayato Matsuo ◽  
Kaho Matsushita ◽  
Ayano Fukuzumi ◽  
Naoki Tokumasu ◽  
Erika Yano ◽  
...  
Keyword(s):  
Safety Assessment ◽  
Genetically Modified ◽  
Protein Profiles ◽  
Serum Ige ◽  
Reactive Protein ◽  
Ige Reactivity ◽  
Gm Soybean ◽  
Genetically Modified Soybeans ◽  
Cp4 Epsps ◽  
Soybean Allergen

Several analyses of allergen levels have been reported as part of the safety assessment of genetically modified (GM) soybean; however, few comprehensive analyses have included new allergens. Thus, in this study the levels of eight major soybean allergens, including Gly m 7 (a newly reported soybean allergen), were semi-quantitatively detected in six GM soybeans and six non-GM soybeans using antigen-immobilized ELISA and immunoblotting. We also analyzed the IgE-reactivity to these soybeans through immunoblotting, using sera from three soybean-allergic patients. The results showed that there were no significant differences in the levels of the major soybean allergens in the GM and non-GM soybeans. Moreover, there were no significant differences in the serum IgE-reactive protein profiles of the patients, as analyzed using immunoblotting. These results indicate that, in general, CP4-EPSPS-transfected GM soybeans are not more allergenic than non-GM soybeans.

scholarly journals Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard

2006 ◽  
Vol 70 (4) ◽  
pp. 821-827 ◽  
Author(s):  
Akie TOYOTA ◽  
Hiroshi AKIYAMA ◽  
Mitsunori SUGIMURA ◽  
Takahiro WATANABE ◽  
Hiroyuki KIKUCHI ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Reference Standard ◽  
Capillary Type ◽  
Genetically Modified Soybeans

Oligonucleotide aptamers: emerging affinity probes for bioanalytical mass spectrometry and biomarker discovery

2015 ◽  
Vol 7 (18) ◽  
pp. 7416-7430 ◽  
Author(s):  
Basri Gülbakan
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Discovery ◽  
Bioanalytical Mass Spectrometry ◽  
Affinity Probes

Aptamers have great potential in affinity mass spectrometry to improve selectivity, specificity and throughput. This review discusses the bioanalytical mass spectrometry and biomarker discovery applications of aptamers.

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