Mass spectrometry combined with affinity probes for the identification of CP4 EPSPS in genetically modified soybeans

2019 ◽  
Vol 55 (2) ◽  
pp. e4371
Author(s):  
Shobha Devi ◽  
Pei-Yu Chu ◽  
Bo-Hung Wu ◽  
Yen-Peng Ho
Keyword(s):  
Mass Spectrometry ◽  
Genetically Modified ◽  
Affinity Probes ◽  
Genetically Modified Soybeans ◽  
Cp4 Epsps

scholarly journals Serum testing of genetically modified soybeans with special emphasis on potential allergenicity of the heterologous protein CP4 EPSPS

2007 ◽  
Vol 51 (8) ◽  
pp. 946-955 ◽  
Author(s):  
Michael Hoff ◽  
Dae-Yeul Son ◽  
Michaela Gubesch ◽  
Kangmo Ahn ◽  
Sang-Il Lee ◽  
...  
Keyword(s):  
Heterologous Protein ◽  
Genetically Modified ◽  
Serum Testing ◽  
Genetically Modified Soybeans ◽  
Cp4 Epsps

scholarly journals Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 522 ◽  
Author(s):  
Ayato Matsuo ◽  
Kaho Matsushita ◽  
Ayano Fukuzumi ◽  
Naoki Tokumasu ◽  
Erika Yano ◽  
...  
Keyword(s):  
Safety Assessment ◽  
Genetically Modified ◽  
Protein Profiles ◽  
Serum Ige ◽  
Reactive Protein ◽  
Ige Reactivity ◽  
Gm Soybean ◽  
Genetically Modified Soybeans ◽  
Cp4 Epsps ◽  
Soybean Allergen

Several analyses of allergen levels have been reported as part of the safety assessment of genetically modified (GM) soybean; however, few comprehensive analyses have included new allergens. Thus, in this study the levels of eight major soybean allergens, including Gly m 7 (a newly reported soybean allergen), were semi-quantitatively detected in six GM soybeans and six non-GM soybeans using antigen-immobilized ELISA and immunoblotting. We also analyzed the IgE-reactivity to these soybeans through immunoblotting, using sera from three soybean-allergic patients. The results showed that there were no significant differences in the levels of the major soybean allergens in the GM and non-GM soybeans. Moreover, there were no significant differences in the serum IgE-reactive protein profiles of the patients, as analyzed using immunoblotting. These results indicate that, in general, CP4-EPSPS-transfected GM soybeans are not more allergenic than non-GM soybeans.

Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)

2009 ◽  
Vol 40 (12) ◽  
pp. 1350-1357 ◽  
Author(s):  
Tao Ran ◽  
Liu Mei ◽  
Wang Lei ◽  
Liu Aihua ◽  
He Ru ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Genetically Modified ◽  
Roundup Ready ◽  
Genetically Modified Soybeans

scholarly journals Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard

2006 ◽  
Vol 70 (4) ◽  
pp. 821-827 ◽  
Author(s):  
Akie TOYOTA ◽  
Hiroshi AKIYAMA ◽  
Mitsunori SUGIMURA ◽  
Takahiro WATANABE ◽  
Hiroyuki KIKUCHI ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Reference Standard ◽  
Capillary Type ◽  
Genetically Modified Soybeans

Oligonucleotide aptamers: emerging affinity probes for bioanalytical mass spectrometry and biomarker discovery

2015 ◽  
Vol 7 (18) ◽  
pp. 7416-7430 ◽  
Author(s):  
Basri Gülbakan
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Discovery ◽  
Bioanalytical Mass Spectrometry ◽  
Affinity Probes

Aptamers have great potential in affinity mass spectrometry to improve selectivity, specificity and throughput. This review discusses the bioanalytical mass spectrometry and biomarker discovery applications of aptamers.

Detection of genetically modified soybeans by PCR method and immunoassay kits

2020 ◽  
Vol 9 (3) ◽  
Author(s):  
H.-Y. Lin ◽  
J.-W. Chiang ◽  
D.Y.-C. Shih
Keyword(s):  
Genetically Modified ◽  
Pcr Method ◽  
Genetically Modified Soybeans

Study of PCR detection methods for genetically modified soybeans with reference molecules

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
H.-Y. Lin ◽  
H.-A. Wei ◽  
F.-P. Lin ◽  
D.Y.-C. Shih
Keyword(s):  
Genetically Modified ◽  
Pcr Detection ◽  
Detection Methods ◽  
Genetically Modified Soybeans
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