Correlation of high-risk human papillomavirus genotypes persistence and risk of residual or recurrent cervical disease after surgical treatment

Abstract BackgroundHuman papillomavirus screen in female cervical cells has demonstrated values in clinical diagnosis of precancerous lesions and cervical cancers. Human papillomavirus tests of cervical cells by utilizing Polymerase Chain Reaction (PCR) method provides human papillomavirus infection status however no further virus in situ information. Although it is well known that the tests of human papillomavirus E6/E7 RNA location in infected cervical cells and cell internal malignancy molecular will provide clues for gynecologists to evaluate disease progression, there are technique difficulties to preserve RNAs in cervical scraped cells for in situ hybridization. MethodsIn current study, after developing a cervical cell collection and preparation methods for RNA in situ hybridization, we captured the chance to screen 98 patient cervical cell samples and detected human papillomavirus E6/E7 mRNAs of high-risk subtypes, low-risk subtypes and lncRNA TERC in the cells. ResultsThere are 70% consistence between human papillomavirus PCR and human papillomavirus RNA in situ hybridization results in cervical collected cells. Viral E6/E7 mRNAs were observed to distribute in cervical cell nuclear and cytoplasm. Moreover, viral gathered clusters were observed outside of cells through human papillomavirus RNA in situ hybridization. Varied numbers of human papillomavirus infective cells were detected by RNAscope assay in different patients even though they are all human papillomavirus high-risk subtype positive discovered by human papillomavirus PCR results. A cell malignancy related long non-coding RNA, TERC, has been detected in seven patient samples. The patient follow-up information was further analyzed with RNAscope results which indicated a combination of RNAscope positive signals of TERC and human papillomavirus high risk signals in more than 10 cells (cytoplasm or nucleus) may connect with cervical lesion fast progression which deserves further studies in the future.ConclusionsTaken together, current study has provided an observable clue for gynecologists to evaluate human papillomavirus infection stage and cell malignancy status which may contribute for assessment of cervical disease progression.

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Risk Factors for High-Risk Human Papillomavirus Infection and Cofactors for High-Grade Cervical Disease in Peru

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e3182288104 ◽

2011 ◽

Vol 21 (9) ◽

pp. 1654-1663 ◽

Cited By ~ 14

Author(s):

Maribel Almonte ◽

Catterina Ferreccio ◽

Miguel Gonzales ◽

Jose Manuel Delgado ◽

C. Hilary Buckley ◽

...

Keyword(s):

Risk Factors ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

High Grade ◽

Papanicolaou Test ◽

First Sexual Intercourse ◽

Papillomavirus Infection ◽

Cervical Disease ◽

Potential Risk Factors

ObjectiveTo evaluate the association between potential risk factors for high-risk human papillomavirus (HR-HPV) infection and cofactors for cervical intraepithelial lesions grade 2 or worse (CIN2+) in women attending cervical screening in Amazonian Peru.Materials and MethodsParticipants completed a risk factor questionnaire before screening. High-risk human papillomavirus infection was determined by Hybrid Capture II. Logistic regression was used to evaluate associations between potential risk factors for HR-HPV infection and between cofactors and risk of CIN2+ among women with HR-HPV infection.ResultsScreening and questionnaires were completed by 5435 women aged 25 to 49 years. The prevalence of HR-HPV was 12.6% (95% confidence interval [CI], 11.8%–13.6%) and decreased by age. Early age at first sexual intercourse and several lifetime sexual partners increased the risk of having HR-HPV (age-adjusted odds ratio [AOR] of age at first sexual intercourse <18 vs ≥20, 1.5; 95% CI, 1.2–2.0; AOR of ≥5 lifetime sexual partners vs 1, 2.1; 95% CI, 1.4–3.2). Among women with HR-HPV infection, those with no schooling (AOR relative to 1–5 years of schooling, 3.2; 95% CI, 1.3–8.3) and those with parity ≥3 (AOR relative to parity <3, 2.6; 95% CI, 1.4–4.9) were at increased risk of CIN2+. The effect of parity was stronger for cancer (AOR of parity ≥3 vs <3, 8.3; 95% CI, 1.0–65.6). Further analysis showed that the association between parity and CIN2+ was restricted to women younger than 40. Most women (83%) had previously been screened. Sixty-four percent of CIN2+ cases detected in this study occurred in women who reported having had a Papanicolaou test in the previous 3 years. Only 4 of 20 cancers were detected in women never screened before. Having had a previous abnormal Papanicolaou test increased the risk of CIN2+ (OR, 16.1; 95% CI, 6.2–41.9).ConclusionAmong women with HR-HPV, high parity (in young women), no schooling, lack of good-quality screening and of adequate follow-up care are the main risk factors for high-grade cervical disease in Peru.

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Abstract B77: High-risk human papillomavirus genotypes among HIV-negative black women with or without cervical disease in South Africa

10.1158/1940-6207.prev-10-b77 ◽

2010 ◽

Author(s):

Alicia C. McDonald ◽

Louise Kuhn ◽

Lynette Denny ◽

Thomas C. Wright

Keyword(s):

South Africa ◽

Human Papillomavirus ◽

Black Women ◽

High Risk ◽

Cervical Disease ◽

Hiv Negative

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Prediction of high-grade cervical disease with human papillomavirus detection in women with glandular and squamous cytologic abnormalities

International Journal of Gynecological Cancer ◽

10.1136/ijgc-00009577-200605000-00017 ◽

2006 ◽

Vol 16 (3) ◽

pp. 1055-1062 ◽

Cited By ~ 1

Author(s):

E. R.Z.M. De Oliveira ◽

S. F.M. Derchain ◽

L. O.Z. Sarian ◽

S. H. Rabelo-Santos ◽

R. C. Gontijo ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Pap Smears ◽

High Grade ◽

Strongly Correlated ◽

Cervical Lesions ◽

Cervical Disease ◽

Glandular Cells ◽

Atypical Glandular Cells ◽

High Risk Hpv

The objective of this study was to assess whether human papillomavirus (HPV) detection with hybrid capture II (HC II) can help predict the presence and the nature, glandular or squamous, of histologic cervical lesions in women referred due to atypical glandular cells (AGC) or high-grade squamous intraepithelial lesion (HSIL). A total of 247 women were included. Referral Pap smears comprised AGC (51 cases), AGC plus HSIL (28 cases), adenocarcinoma in situ (10 cases), and HSIL (158 cases). All patients were tested for high-risk HPV with HC II and had a histologic assessment of their cervix. Histologic analysis showed 38 women with (15.3%) cervicitis, 194 with (75.5%) squamous lesions, and 15 with (9.2%) glandular neoplasia. The overall rate of high-risk HPV detection was 77%. Almost 70% of AGC-HPV–negative patients did not have a pathologically proven cervical neoplasia, whereas 76% of women with AGC-HPV–positive result were diagnosed with a squamous or glandular neoplasia. Most (95%) of the lesions in patients with AGC-HSIL were of squamous nature, and HPV detection did not contribute to their differentiation from glandular lesions. We conclude that in women with AGC, HPV positivity strongly correlated with the presence of glandular or squamous cervical lesion but did not help distinguishing women with squamous from those with glandular neoplasia.

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