Molecular epidemiology of noroviruses in children under 5 years of age with acute gastroenteritis in Yaoundé, Cameroon

ABSTRACT BACKGROUND: Norovirus (NoV) is an important etiologic agent of acute gastroenteritis and infects individuals of all ages, especially children in Brazil and worldwide. NoV GII.4 was the most prevalent genotype worldwide because of your extensive genetic diversity. In Brazil, especially in the Northeast, few studies have been developed for identify and molecularly characterize NoV. OBJECTIVE: The present study aimed to detect and describe the molecular epidemiology of NoV associated with acute gastroenteritis. METHODS: The viral RNA extracted from stool samples were subjected to Nested RT-PCR and the genotypes were determined by nucleotide sequences analysis. In total, 278 stool samples assisted at Aliança Hospital in the city of Salvador, with acute gastroenteritis were examined, between March 2009 and July 2012. RESULTS: A high NoV rate (54.2%) was identified in children under 5 years of age. We detected the circulation of different NoV GII.4 variants in Salvador, during the study period as Den Haag 2006b, New Orleans 2009 and Sydney 2012. CONCLUSION: These findings reinforce the need to study the molecular epidemiology of NoV infections in acute gastroenteritis.

Download Full-text

Molecular epidemiology and associated risk factors of rotavirus infection among children under 5 years hospitalized for acute gastroenteritis in North Eastern, Kenya, 2012

Pan African Medical Journal ◽

10.11604/pamj.supp.2017.28.1.2486 ◽

2017 ◽

Vol 28 ◽

Cited By ~ 1

Author(s):

Ahmed Mohamed Fidhow ◽

Amwayi Samwel ◽

Zipporah Ng’ang’a ◽

Joseph Oundo ◽

James Nyangao ◽

...

Keyword(s):

Risk Factors ◽

Molecular Epidemiology ◽

Acute Gastroenteritis ◽

Rotavirus Infection ◽

North Eastern ◽

Associated Risk Factors ◽

Children Under 5

Download Full-text

Molecular epidemiology of norovirus GII.4 variants in children under 5 years with sporadic acute gastroenteritis in South Korea during 2006–2013

Journal of Clinical Virology ◽

10.1016/j.jcv.2014.08.018 ◽

2014 ◽

Vol 61 (3) ◽

pp. 340-344 ◽

Cited By ~ 13

Author(s):

Han-Gil Cho ◽

Sung-Geun Lee ◽

Ju-Eun Kim ◽

Kyeong-Sin Yu ◽

Deog-Yong Lee ◽

...

Keyword(s):

South Korea ◽

Molecular Epidemiology ◽

Acute Gastroenteritis ◽

Norovirus Gii ◽

Sporadic Acute Gastroenteritis ◽

Children Under 5

Download Full-text

Molecular epidemiology and genetic diversity of norovirus infection in children with acute gastroenteritis in Bangladesh, 2014‐2019

Journal of Medical Virology ◽

10.1002/jmv.26772 ◽

2021 ◽

Author(s):

Shuvra Kanti Dey ◽

Nadim Sharif ◽

Baki Billah ◽

Tanjir Tarek Ibn Siddique ◽

Tarequl Islam ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Epidemiology ◽

Acute Gastroenteritis

Download Full-text

Molecular epidemiology of norovirus from patients with acute gastroenteritis in northwestern Spain

Epidemiology and Infection ◽

10.1017/s0950268814000740 ◽

2014 ◽

Vol 143 (2) ◽

pp. 316-324 ◽

Cited By ~ 7

Author(s):

C. F. MANSO ◽

J. L. ROMALDE

Keyword(s):

Phylogenetic Analysis ◽

Molecular Epidemiology ◽

Genetic Drift ◽

Acute Gastroenteritis ◽

Age Groups ◽

Capsid Gene ◽

High Prevalence ◽

High Incidence ◽

Northwestern Spain

SUMMARYThe high incidence of norovirus (NoV) infections seems to be related to the emergence of new variants that evolved by genetic drift of the capsid gene. In this work, that represents a first effort to describe the molecular epidemiology of NoV in the northwest of Spain, a total of eight different NoV genotypes (GII.1, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14) were detected. The major genotypes observed were GII.4 (45·42%) and GII.14 (34·9%), being detected in all age groups. In addition, and although most of GII.4 sequences belonged to 2006b (7·2%) and 2010 (50·35%) variants, the presence of new NoV variants was observed. Phylogenetic analysis revealed that a high number of GII.4 sequences (35·24%) could be assigned to the newly emerging Sydney 2012 variant, even during late 2010. The high prevalence of NoV GII.14 observed in this study may indicate the emergence of this genotype in Spain.

Download Full-text

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Pathogens ◽

10.3390/pathogens10060674 ◽

2021 ◽

Vol 10 (6) ◽

pp. 674

Author(s):

Anton Yuzhakov ◽

Ksenia Yuzhakova ◽

Nadezhda Kulikova ◽

Lidia Kisteneva ◽

Stanislav Cherepushkin ◽

...

Keyword(s):

Infectious Disease ◽

Genetic Diversity ◽

Acute Gastroenteritis ◽

Rt Pcr ◽

Clinical Hospital ◽

Group A Rotavirus ◽

Group A ◽

First Time ◽

Common Combination ◽

Children Under 5

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

Download Full-text