The effect of mechanical stress on enthesis homeostasis in a rat Achilles enthesis organ culture model

2021 ◽  
Author(s):  
Taichi Saito ◽  
Ryo Nakamichi ◽  
Aki Yoshida ◽  
Takaaki Hiranaka ◽  
Yuki Okazaki ◽  
...  
Keyword(s):  
Organ Culture ◽  
Mechanical Stress ◽  
Culture Model

scholarly journals Programmed NP Cell Death Induced by Mitochondrial ROS in a One-Strike Loading Disc Degeneration Organ Culture Model

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Bao-Liang Li ◽  
Xizhe Liu ◽  
Manman Gao ◽  
Fu Zhang ◽  
Xu Chen ◽  
...  
Keyword(s):  
Cell Death ◽  
Organ Culture ◽  
Cell Viability ◽  
Mechanical Stress ◽  
Disc Degeneration ◽  
Early Time ◽  
Time Points ◽  
Physiological Loading ◽  
Mitochondrial Ros ◽  
Culture Model

Increasing evidence has indicated that mitochondrial reactive oxygen species (ROS) play critical roles in mechanical stress-induced lumbar degenerative disc disease (DDD). However, the detailed underlying pathological mechanism needs further investigation. In this study, we utilized a one-strike loading disc degeneration organ culture model to explore the responses of intervertebral discs (IVDs) to mechanical stress. IVDs were subjected to a strain of 40% of the disc height for one second and then cultured under physiological loading. Mitoquinone mesylate (MitoQ) or other inhibitors were injected into the IVDs. IVDs subjected to only physiological loading culture were used as controls. Mitochondrial membrane potential was significantly depressed immediately after mechanical stress ( P < 0.01 ). The percentage of ROS-positive cells significantly increased in the first 12 hours after mechanical stress and then declined to a low level by 48 hours. Pretreatment with MitoQ or rotenone significantly decreased the proportion of ROS-positive cells ( P < 0.01 ). Nucleus pulposus (NP) cell viability was sharply reduced at 12 hours after mechanical stress and reached a stable status by 48 hours. While the levels of necroptosis- and apoptosis-related markers were significantly increased at 12 hours after mechanical stress, no significant changes were observed at day 7. Pretreatment with MitoQ increased NP cell viability and alleviated the marker changes by 12 hours after mechanical stress. Elevated mitochondrial ROS levels were also related to extracellular matrix (ECM) degeneration signs, including catabolic marker upregulation, anabolic marker downregulation, increased glycosaminoglycan (GAG) loss, IVD dynamic compressive stiffness reduction, and morphological degradation changes at the early time points after mechanical stress. Pretreatment with MitoQ alleviated some of these degenerative changes by 12 hours after mechanical stress. These changes were eliminated by day 7. Taken together, our findings demonstrate that mitochondrial ROS act as important regulators of programmed NP cell death and ECM degeneration in IVDs at early time points after mechanical stress.

scholarly journals A novel organ culture model of aorta for vascular calcification

2016 ◽  
Vol 244 ◽  
pp. 51-58 ◽  
Author(s):  
Takuyu Akiyoshi ◽  
Hidetaka Ota ◽  
Katsuya Iijima ◽  
Bo-Kyung Son ◽  
Tomoaki Kahyo ◽  
...  
Keyword(s):  
Organ Culture ◽  
Vascular Calcification ◽  
Culture Model

scholarly journals A new nondestructive cytometric assay based on resazurin metabolism and an organ culture model for the assessment of corneal viability

Cytometry ◽  
2003 ◽  
Vol 55A (1) ◽  
pp. 7-14 ◽  
Author(s):  
S�bastien Perrot ◽  
H�l�ne Dutertre-Catella ◽  
Chantal Martin ◽  
Jean-Michel Warnet ◽  
Patrice Rat
Keyword(s):  
Organ Culture ◽  
Culture Model

scholarly journals Effects of Asymmetric Dynamic Loading on Intervertebral Disc – towards a Scoliosis Mimicking Organ Culture Model

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582636-s-0036-1582636
Author(s):  
Zhen Li ◽  
Ying Zhang ◽  
Lukas Straumann ◽  
Patrick Lezuo ◽  
Marianna Peroglio ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Dynamic Loading ◽  
Culture Model

An organ culture model for assaying wound repair of the fibrocartilaginous knee joint meniscus

1989 ◽  
Vol 17 (3) ◽  
pp. 393-400 ◽  
Author(s):  
Richard J. Webber ◽  
J. Lyndal York ◽  
John L. Vanderschilden ◽  
Aubrey J. Hough
Keyword(s):  
Knee Joint ◽  
Organ Culture ◽  
Wound Repair ◽  
Culture Model

A human osteoarthritis osteochondral organ culture model for cartilage tissue engineering

Biomaterials ◽  
2018 ◽  
Vol 162 ◽  
pp. 1-21 ◽  
Author(s):  
P. Yeung ◽  
W. Zhang ◽  
X.N. Wang ◽  
C.H. Yan ◽  
B.P. Chan
Keyword(s):  
Tissue Engineering ◽  
Organ Culture ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Culture Model

Pressure and Angiotensin II Synergistically Induce Aortic Fibronectin Expression in Organ Culture Model of Rabbit Aorta

1996 ◽  
Vol 79 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Nathalie Bardy ◽  
Re´gine Merval ◽  
Joe¨lle Benessiano ◽  
Jane-Lyse Samuel ◽  
Alain Tedgui
Keyword(s):  
Angiotensin Ii ◽  
Organ Culture ◽  
Rabbit Aorta ◽  
Culture Model ◽  
Fibronectin Expression
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