Quantification of growth factors in advanced platelet‐rich fibrin and concentrated growth factors and their clinical efficacy as adjunctive to the GTR procedure in periodontal intrabony defects

Platelet concentrates (PCs) have been used for over 20 years in dentistry, as an adjunct to oral surgery procedures, to improve hard and soft tissue healing and control postoperative symptoms. Among various PCs, Leukocyte and Platelet-Rich Fibrin (L-PRF) has become very popular due to its excellent cost-effectiveness ratio, and to the simple preparation protocol, but comparative clinical studies with other PCs are lacking. The aim of this split-mouth cohort study was to evaluate the effect of Concentrated Growth Factors (CGF), a recently introduced PC, as compared to L-PRF for enhancing post-extraction socket healing. Methods: Patients in need of bilateral tooth extractions were included. Each side was treated with either CGF or L-PRF. Pain, socket closure and healing index were the main outcomes. Results: Forty-five patients (24 women), aged 60.52 ± 11.75 years (range 37–87 years) were treated. No significant difference in outcomes was found, except for Pain at day 1 (p < 0.001) and socket closure in the vestibulo-palatal/lingual dimension at day 7 post-extraction (p = 0.04), both in favor of CGF. Conclusions: based on the present results, CGF proved to be as effective and safe as L-PRF, representing a valid alternative option for improving alveolar socket healing and reducing postoperative discomfort.

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The effect of concentrated growth factors in the treatment of periodontal intrabony defects

Future Science OA ◽

10.4155/fsoa-2016-0019 ◽

2016 ◽

Vol 2 (4) ◽

Cited By ~ 10

Author(s):

Jing Qiao ◽

Jinyu Duan ◽

Yong Zhang ◽

Yi Chu ◽

Changzhou Sun

Keyword(s):

Growth Factors ◽

Intrabony Defects ◽

Concentrated Growth Factors

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The effectiveness of platelet concentrations in periodontal surgeries

International Journal of Dental Research ◽

10.14419/ijdr.v6i2.12414 ◽

2018 ◽

Vol 6 (2) ◽

pp. 61 ◽

Cited By ~ 1

Author(s):

Ronad Al-Azem ◽

Neveen Ali ◽

Diana Mostafa

Keyword(s):

Case Report ◽

Growth Factors ◽

Bone Graft ◽

Tissue Regeneration ◽

Platelet Rich Plasma ◽

Research Process ◽

Platelet Concentrates ◽

Platelet Rich Fibrin ◽

Barrier Membrane ◽

Concentrated Growth Factors

Platelets release several growth factors which stimulate tissue regeneration. Several techniques for platelet concentrates such as platelet rich plasma (PRP), plasma rich in growth factors (PRGF), platelet rich fibrin (PRF) and concentrated growth factors (CGF) have been introduced in dental surgeries for the prevention of hemorrhage and acceleration of tissue regeneration. However, a fabricating growth factors-enriched bone graft matrix which is called “sticky bone” has been demonstrated to provide stabilization in bony defects. In this article, we presented the method of preparing and utilizing CGF and sticky bone and evaluate the effect of CGF mixed with bone graft and CGF barrier membrane in periodontal surgeries.Methodology: We used websites such as PubMed, Scopus, and ISI Web of knowledge to get related articles about this subject. The research process involved specific key words " concentrated growth factor”- “Platelet rich fibrin”- growth factors-enriched bone graft” - “sticky bone” to find more articles which published from 2007 to March 2018.Results: We reviewed 48 articles, 43 articles were excluded. Only five articles have been conducted. Original human studies and case report were included.Conclusion: We concluded that the use of sticky bone and CGF is effective in bone grafting and implant.

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Quantitative Near-Infrared Imaging of Platelets in Platelet-Rich Fibrin (PRF) Matrices: Comparative Analysis of Bio-PRF, Leukocyte-Rich PRF, Advanced-PRF and Concentrated Growth Factors

International Journal of Molecular Sciences ◽

10.3390/ijms21124426 ◽

2020 ◽

Vol 21 (12) ◽

pp. 4426 ◽

Cited By ~ 6

Author(s):

Hachidai Aizawa ◽

Tetsuhiro Tsujino ◽

Taisuke Watanabe ◽

Kazushige Isobe ◽

Yutaka Kitamura ◽

...

Keyword(s):

Growth Factors ◽

Near Infrared ◽

Infrared Imaging ◽

Buffy Coat ◽

Gradient Theory ◽

Blood Samples ◽

Platelet Rich Fibrin ◽

Plasma Fraction ◽

Distal Surface ◽

Concentrated Growth Factors

Platelet-rich fibrin (PRF) is a fibrin matrix enriched with platelets. The PRF matrix is thought to form a steep gradient of platelet density around the region corresponding to the buffy coat in anticoagulated blood samples. However, this phenomenon has not yet been proven. To visualize platelet distribution in PRF in a non-invasive manner, we utilized near-infrared (NIR) imaging technology. In this study, four types of PRF matrices, bio-PRF, advanced-PRF (A-PRF), leukocyte-rich PRF (L-PRF), and concentrated growth factors (CGF) were compared. Blood samples collected from healthy, non-smoking volunteers were immediately centrifuged using four different protocols in glass tubes. The fixed PRF matrices were sagittally divided into two equal parts, and subjected to modified immunohistochemical examination. After probing with NIR dye-conjugated secondary antibody, the CD41+ platelets were visualized using an NIR imager. In L-PRF and CGF, platelets were distributed mainly on and below the distal surface, while in bio-PRF and A-PRF, platelet distribution was widespread and homogenous. Among three regions of the PRF matrices (upper, middle, and lower), no significant differences were observed. These findings suggest that platelets aggregate on polymerizing fibrin fibers and float up as a PRF matrix into the plasma fraction, amending the current “gradient” theory of platelet distribution.

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