Study of the Color Reaction of Streptomycin Sulfate with Procaine Hydrochloride

1962 ◽  
Vol 51 (5) ◽  
pp. 496-497 ◽  
Author(s):  
A.V. Puccini ◽  
A.J. Spiegel
Keyword(s):  
Color Reaction ◽  
Procaine Hydrochloride ◽  
Streptomycin Sulfate

Intestinal absorption of coprostanol (coprosterol) in the rat

1960 ◽  
Vol 199 (6) ◽  
pp. 1008-1010 ◽  
Author(s):  
C.S. Narayana Setty ◽  
A. C. Ivy
Keyword(s):  
Oleic Acid ◽  
Intestinal Absorption ◽  
Chemical Structure ◽  
Control Period ◽  
Chair Conformation ◽  
Color Reaction ◽  
Salt Mixture ◽  
Streptomycin Sulfate

A basal sterol-free diet consisting of casein, 18%; dextrin, 57%; oleic acid, 9%; U.S.P. salt mixture, 2%; yeast, 5%; and cellulose, 9%; along with 1% sulfasuxidine and 0.2% streptomycin sulfate was fed to eight rats during a control period of 12 days, the feces being collected under alcohol the last 4 days and assayed for digitonide sterol. The experiment was repeated with the addition of 28 mg of coprostanol to the diet. It was found that from 41 to 61% (average 48%) of the coprostanol was absorbed. The relation of chemical structure to the absorbability of cholesterol derivatives is discussed with the finding that all absorbable cholesterol derivatives have the ‘chair’ conformation, but are not alike in digitonide formation and Liebermann-Burchard color reaction.

Development of the Foramen Secundum in the Chick as Observed by Scanning Electron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 212-213
Author(s):  
M.J.C. Hendrix ◽  
D.E. Morse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Procaine Hydrochloride ◽  
Cardiac Anomaly ◽  
Chick Embryos ◽  
Congenital Cardiac Anomaly ◽  
Septal Defects ◽  
Critical Point Drying ◽  
Scanning Electron

Atrial septal defects are considered the most common congenital cardiac anomaly occurring in humans. In studying the normal sequential development of the atrial septum, chick embryos of the White Leghorn strain were prepared for scanning electron microscopy and the results were then extrapolated to the human heart. One-hundred-eighty chick embryos from 2 to 21 days of age were removed from their shells and immersed in cold cacodylate-buffered aldehyde fixative . Twenty-four embryos through the first week post-hatching were perfused in vivo using cold cacodylate-buffered aldehyde fixative with procaine hydrochloride. The hearts were immediately dissected free and remained in the fixative a minimum of 2 hours. In most cases, the lateral atrial walls were removed during this period. The tissues were then dehydrated using a series of ascending grades of ethanol; final dehydration of the tissues was achieved via the critical point drying method followed by sputter-coating with goldpalladium.

The Development of a Turbidimetric Method for the Quantitative Determination of Antibiotics of the Aminoglycoside Group in Medications

2020 ◽  
Vol 65 (7-8) ◽  
pp. 37-41
Author(s):  
E. N. Semenova ◽  
S. I. Kuleshova ◽  
E. I. Sakanyan
Keyword(s):  
Quantitative Determination ◽  
Quality Assessment ◽  
Aminoglycoside Antibiotics ◽  
Metrological Characteristics ◽  
Streptomycin Sulfate ◽  
Turbidimetric Method ◽  
Validation Criteria ◽  
Validation Parameters ◽  
Validation Tests

A method for the quantitative determination of streptomycin sulfate in medicines by the turbidimetric method has been developedand validated. Based on the results of the experiments, it was found that the metrological characteristics of such validation parameters of the method as linearity, precision, and correctness do not exceed the validation criteria. Linearity was noted in the range of streptomycin concentrations from 3.75 to 8.43 μg/ml. The results of validation tests of the method for the quantitative determination of streptomycin indicate the prospects and feasibility of introducing the turbidimetric method into the domestic system for standardization and quality assessment of aminoglycoside antibiotics.

Direct estimation of lysine in corn meals by the ninhydrin color reaction

1975 ◽  
Vol 23 (2) ◽  
pp. 194-196 ◽  
Author(s):  
Alfred C. Beckwith ◽  
Jerrold W. Paulis ◽  
Joseph S. Wall
Keyword(s):  
Color Reaction ◽  
Direct Estimation

Evaluation of Trinder's Glucose Oxidase Method for Measuring Glucose in Serum and Urine

1975 ◽  
Vol 21 (12) ◽  
pp. 1754-1760 ◽  
Author(s):  
John A Lott ◽  
Kathie Turner
Keyword(s):  
Glucose Oxidase ◽  
Oxidase Activity ◽  
Color Reaction ◽  
Critical Variable ◽  
Wide Range ◽  
Serum And Urine ◽  
Good Agreement

Abstract Trinder's method for glucose has nearly all the attributes of an ideal automated colorimetric glucose oxidase procedure. The chemicals used in the color reaction with peroxidase are readily available, the solutions are stable and can be prepared by the user, the method is highly specific and largely free of interferences, the sensitivity can be adjusted by the user to cover a wide range of glucose concentrations, and the reagents are not hazardous. We found very good agreement between results by this method and by the hexokinase and Beckman Glucose Analyzer methods. The method has been modified and adapted to the AutoAnalyzer I and SMA 6/60 (Technicon) with manifolds that give very little interaction between specimens. A study of the method by the simplex technique revealed that the glucose oxidase activity in the reagent is the most critical variable.

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