Evaluation of compositional and nutritional equivalence of genetically modified rice to conventional rice using in situ and in vitro techniques

2009 ◽  
Vol 89 (9) ◽  
pp. 1490-1497 ◽  
Author(s):  
Bo Lin ◽  
Zhiliang Tan ◽  
Guoying Xiao ◽  
Min Wang ◽  
Zhihui Cong ◽  
...  
2020 ◽  
Vol 158 (1-2) ◽  
pp. 150-158 ◽  
Author(s):  
B. C. Silva ◽  
M. V. C. Pacheco ◽  
L. A. Godoi ◽  
F. A. S. Silva ◽  
D. Zanetti ◽  
...  

AbstractAn experiment was conducted to evaluate: (1) the effects of ensiling maize or sorghum grains after reconstitution on readily soluble fraction (a), potentially degradable fraction in the rumen (b) and rate constant for degradation of b (c) of dry matter (DM), organic matter (OM) and starch (STA); and (2) an appropriate incubation time for in situ or in vitro procedures to estimate in vivo digestibility. Four rumen-cannulated Nellore bulls (body weight = 262 ± 19.6 kg) distributed in a 4 × 4 Latin square were used. Diets were based on dry ground maize (DGM); or dry ground sorghum (DGS); or reconstituted ground maize silage; or reconstituted ground sorghum silage. In vitro and in situ incubations of the individual grains and diets were simultaneously performed with in vivo digestibility. In general, reconstituted grains and diets based on reconstituted grains presented greater (P < 0.05) fraction a and lower (P < 0.05) fraction b of DM, OM and STA compared to dry grains and diets based on dry grain. However, the magnitude of response of the reconstitution and ensiling process on DM and OM degradability parameter was greater for maize than that for sorghum. Moreover, no differences (P > 0.05) were observed between DGM- and DGS-based diets for c estimates. The results suggest that the reconstitution process promotes grains protein matrix breakdown increasing STA availability. The incubation times required for in vivo digestibility estimations of DM, OM and STA are 24 h for in situ and 36 h for in vitro procedures.


2012 ◽  
Vol 175 (3-4) ◽  
pp. 95-105 ◽  
Author(s):  
B. Edmunds ◽  
K.-H. Südekum ◽  
H. Spiekers ◽  
F.J. Schwarz

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Salah E. Maaty ◽  
Ahmed Sh. Hassan ◽  
Mamdouh K. Amin ◽  
Mohamed M.A. Elashtokhy

The present study aimed to study the fitness between a trans-conjugant (recombinant strain) of Pseudomonas aeruginosa bacteria with its parents after transferring genetic material by conjugation mechanism. Whereas, environmental fitness expresses the interaction of an organism with its environment therefore it is considered a good indicator for the assessment of genetically engineered microorganisms (GEM) released into nature. Incubation time was carried out in vitro and incubating time in situ in Mowas River Zagazig city during winter and summer. Accordingly, the fitness of the parents and the recombinant strain was studied. The three strains of Pseudomonas aeruginosa (PAO1, MAM2 and PU21) were tested on chloramphenicol and tetracycline. Strain MAM2 was resistant to chloramphenicol 1200 µg/ml while was sensitive to tetracycline and has been used as the recipient. While strain PAO1 was resistant to tetracycline 200 µg/ml and was sensitive to chloramphenicol and has been used as the donor. Results proofed the presence of the plasmid in the donor and trans conjugant strains. The donor was treated with acridine orange to match the results obtained with the results at the molecular level. It was observed that bacterial fitness continued for up to 35 days in vitro, while in situ during the summer it did not last at the site for only 21 days. While it lasted 28 days during the summer. So, the risks that may be caused by releasing the genetically modified microorganisms into environments have been canceled. In addition to its ability to preserve the new genetic material, it may be able to transfer this new genetic material to other strains and species that may be live in the same ecosystem, as it is largely stable in the environment. In genetically modified microorganisms that are added to the environments for agricultural uses such as increasing soil fertility (bio-fertilizer) or biodegradation for a harmful substance such as pesticides, the soil must be re-inoculated in


Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 316
Author(s):  
Francesco Sottile ◽  
Chiara Caltagirone ◽  
Cristiana Peano ◽  
Maria Beatrice Del Signore ◽  
Ettore Barone

As a perennial xerophytic shrub, characterized by plesiomorphic features, the caper (Capparis spinosa L.) is naturally spread throughout the Mediterranean basin and occupies an important ecological role, as well as an economic one, in traditional and specialized systems for commercial production. This species, in spite of its wide diffusion, is currently considered at risk of genetic erosion, mainly due to overgrazing and overharvesting for domestic uses and for trade. This situation is made more serious because of the lack of efficient propagation techniques, determining the caper as a “difficult-to-propagate species”. In this review, we report the main available sexual and vegetative propagation techniques with the aim of assessing whether, and to what extent, this criticality is still true for caper as a horticultural crop. In terms of seed propagation, germination rates have generally been considered quite low or unsatisfactory, and are also affected by hybridization phenomena that are likely to occur among both the wild and cultivated forms. The seeds show a physiological dormancy that can be lowered by adopting hormonal treatments, but in situ germination remains a critical phase. Vegetative propagation appears quite effective, mostly as related to in vitro techniques that allow caper cultivation that is no longer affected by propagation for an economic dissemination of the species in more intensive orchards. The research needs for Caper spinosa L. as a horticultural crop, especially in the field of genetic improvement and breeding, are also underlined.


2004 ◽  
Vol 116 (1-2) ◽  
pp. 35-51 ◽  
Author(s):  
J.M.J Gosselink ◽  
J.P Dulphy ◽  
C Poncet ◽  
J Aufrère ◽  
S Tamminga ◽  
...  
Keyword(s):  

2002 ◽  
Vol 2002 ◽  
pp. 141-141
Author(s):  
Ives C.S. Bueno ◽  
Sergio L.S. Cabral Filho ◽  
Liliana L. Oetting ◽  
Mariana C. Machado ◽  
Sarita P. Gobbo ◽  
...  

In vivo experiments are the preferred method for ruminant feed evaluation, but they are very expensive, laborious and time-consuming. In situ and in vitro techniques are commonly used as a routine all over the world as a predictor of in vivo results. In situ assays have been the basis of many feed evaluation systems due to its ease of use and low cost. In vitro techniques, such as gas production, give an opportunity to get similar information plus a better description of fermentative kinetics. The aim of this work was to compare data obtained from in vivo, in vitro and in situ assays for the evaluation of three tropical forages used in ruminant nutrition in Brazil.


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