Detection of central nervous system tissue as bovine spongiform encephalopathy specified risk material in traditional Turkish meat products

2011 ◽  
Vol 92 (8) ◽  
pp. 1653-1656
Author(s):  
Mehmet Kale ◽  
Sibel Hasırcıoglu ◽  
Cagdas Ozturk ◽  
A Selcen Akcan Kale ◽  
Yusuf Dogruer
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Bovine Spongiform Encephalopathy ◽  
Meat Products ◽  
Spongiform Encephalopathy ◽  
Central Nervous System Tissue ◽  
Specified Risk Material

Comparison of Commercially Available Antibodies for the Detection of Central Nervous System Tissue in Meat Products by Enzyme-Linked Immunosorbent Assay

2005 ◽  
Vol 68 (3) ◽  
pp. 630-632 ◽  
Author(s):  
EVA RENCOVA
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Immunosorbent Assay ◽  
Meat Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spongiform Encephalopathy ◽  
Central Nervous System Tissue ◽  
Human Form ◽  
Tissue Samples ◽  
New Variant

An indirect competitive enzyme-linked immunosorbent assay was used to compare four commercially available monoclonal and polyclonal antibodies prepared against the markers of central nervous system tissue: glial fibrillary acidic protein, neurofilaments, and myelin basic protein. These antibodies were investigated for their usefulness at the detection of prohibited central nervous system tissue in meat products to prevent spread of bovine spongiform encephalopathy and its human form, a new variant of Creutzfeldt-Jakob disease. The detection limit was 2.5 to 10%. The specificity was tested using extracts of tissue samples of bovine and porcine brain, spinal cord, lymphatic node, diaphragm, skeletal muscle, and peripheral nerves. All antibodies reacted with both bovine and porcine tissue extracts.

Control Points To Reduce Movement of Central Nervous System Tissue during Beef Slaughter

2017 ◽  
Vol 80 (2) ◽  
pp. 355-360
Author(s):  
J. L. Aalhus ◽  
R. D. Thacker ◽  
I. L. Larsen ◽  
J. C. Roberts ◽  
M. A. Price ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cold Water ◽  
Effective Means ◽  
Hot Water ◽  
Spongiform Encephalopathy ◽  
Cross Contamination ◽  
Central Nervous System Tissue ◽  
Before And After ◽  
Disease Variant

ABSTRACT Consumption of central nervous system tissue (CNST) from cattle with bovine spongiform encephalopathy (BSE) is thought to cause the human neurological disease, variant Creutzfeldt-Jacob disease. To identify points of cross-contamination of beef carcasses with CNST, 55 young beef cattle were slaughtered and processed through a federally inspected multispecies abattoir. The objectives of this study were to evaluate CNST spread following the placement of a plug in the penetration site of the skull after captive bolt stunning, to evaluate cross-contamination of carcasses before and after splitting, to compare the effects of hot water pasteurization (84°C for 10 s) versus cold water wash (10°C for 30 s) for reducing CNST on the carcass, and to examine other possible sources of cross-contamination in the abattoir. Results indicated that the use of a plastic plug reduced CNST contamination near the bolt penetration site. This study also confirmed that carcass splitting resulted in an increase in CNST contamination at various areas of the carcass. Hot water pasteurization appeared to be an effective means of removing CNST contamination from carcasses in most of the areas sampled.

Comparison of Analytical Methods for the Detection of Central Nervous System Tissue in Ground Beef

2008 ◽  
Vol 71 (7) ◽  
pp. 1505-1509
Author(s):  
DONG GYUN LIM ◽  
CHEORUN JO ◽  
MOOHA LEE
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Nervous System ◽  
Western Blotting ◽  
Ground Beef ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spongiform Encephalopathy ◽  
Spinal Cord Tissue ◽  
Central Nervous System Tissue ◽  
By Products

Bovine spongiform encephalopathy is most likely transmitted by the consumption of central nervous system tissue of infected cows. The objective of this study was to compare the sensitivity or limits of detection of two central nervous system tissue detection assays (glial fibrillary acidic protein enzyme-linked immunosorbent assay [ELISA] and neuron-specific enolase Western blotting assay) in by-products and ground beef. By-products including brain, spinal cord, and ileum were collected from the slaughterhouse and used for analyses with ELISA and Western blotting assays. Beef samples were prepared by mixing ground beef with different amounts of spinal cord tissue (0, 0.03, 0.06, and 0.1%) and were analyzed using the two central nervous system tissue detection methods. Both analytical assays were applicable in detecting central nervous system tissue in ground beef. However, the ELISA method was considered superior because of its ease of use, high sensitivity, and rapidity as compared with the Western blot method.

scholarly journals Protease-resistant PrP deposition in brain and non-central nervous system tissues of a murine model of bovine spongiform encephalopathy

1996 ◽  
Vol 77 (8) ◽  
pp. 1941-1946 ◽  
Author(s):  
C. F. Farquhar ◽  
J. Dornan ◽  
R. C. Moore ◽  
R. A. Somerville ◽  
A. M. Tunstall ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Bovine Spongiform Encephalopathy ◽  
Murine Model ◽  
Spongiform Encephalopathy
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