Effective length calibration method for processing the fluorescence signal detected by charge-coupled device in capillary electrophoresis

2017 ◽  
Vol 40 (9) ◽  
pp. 2054-2061 ◽  
Author(s):  
Yi Ni ◽  
Chenchen Liu ◽  
Jin Chen ◽  
Qinmiao Chen ◽  
Xifang Zhu ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Calibration Method ◽  
Effective Length ◽  
Fluorescence Signal ◽  
Charge Coupled Device

Geometric Calibration Method of Side-Scatter Lidar Based on Charge-Coupled Device

2015 ◽  
Vol 44 (2) ◽  
pp. 201002
Author(s):  
麻晓敏 MA Xiao-min ◽  
史博 SHI Bo ◽  
单会会 SHAN Hui-hui ◽  
赵素贵 ZHAO Su-gui ◽  
陶宗明 TAO Zong-ming
Keyword(s):  
Calibration Method ◽  
Charge Coupled Device ◽  
Geometric Calibration

Defining the Tools: an Analysis of Laser Scanning Confocal and Wide-Field/Restoration Fluorescence Microscope Imaging

2001 ◽  
Vol 7 (S2) ◽  
pp. 1002-1003
Author(s):  
Jason R. Swedlow ◽  
Paul D. Andrews ◽  
Ke Hu ◽  
David S. RoosT ◽  
John M. Murray
Keyword(s):  
Fluorescence Imaging ◽  
Laser Scanning ◽  
Image Point ◽  
Fluorescence Signal ◽  
Wide Field ◽  
Charge Coupled Device ◽  
Standard Tool ◽  
Microscope Imaging ◽  
A Charge ◽  
Cellular Components

Digital fluorescence microscopy is now a standard tool for determining the localization of cellular components in fixed and living cells. Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state. The laser scanning microscope uses a diffraction-limited focused beam to scan the sample and develop an image point by point. in addition, a pinhole placed in a plane confocal to the specimen prevents emitted out-of focus fluorescence from reaching the photomultiplier tube (PMT) detector. By combining spot illumination and selection of infocus fluorescence signal, the laser scanning confocal microscope (LSCM) creates an image of the specimen largely free of out-of-focus blur. By contrast, a wide-field microscope (WFM) illuminates the whole specimen simultaneously and detects the signal with a spatial array of point detectors, usually a charge-coupled device camera (CCD). This approach collects an image of all points of the specimen simultaneously and includes all the out-of-focus blurred light. Subsequent restoration by iterative deconvolution generates an estimate of the specimen, largely free of out-of-focus blur. While many other fluorescence imaging modalities exist, these two methods represent the majority of the fluorescence imaging systems currently in use in biomedical research.

scholarly journals Development of a Capillary Electrophoresis Method for the Enantioselective Estimation of Primaquine in Pharmaceutical Formulations

2008 ◽  
Vol 91 (3) ◽  
pp. 536-541 ◽  
Author(s):  
Abdalla A Elbashir ◽  
Bahruddin Saad ◽  
Abdussalam Salhin Mohamed Ali ◽  
Muhammad Idiris Saleh
Keyword(s):  
Capillary Electrophoresis ◽  
Applied Voltage ◽  
Pharmaceutical Formulations ◽  
Fused Silica ◽  
Chiral Selector ◽  
Effective Length ◽  
Racemic Mixture ◽  
Injection Time ◽  
Relative Standard ◽  
Capillary Temperature

Abstract A capillary electrophoresis (CE) method has been developed that allows the separation and estimation of primaquine enantiomers using hydroxypropyl--cyclodextrin (HP--CD) as a chiral selector. The influence of chemical and instrumental parameters on the separation, such as type and concentration of CD, buffer concentration, buffer pH, applied voltage, capillary temperature, and injection time, were investigated. Good separation of the racemic mixture of primaquine was achieved using a fused-silica capillary (52.5 cm effective length 50 m id) and a background electrolyte composed of tris-phosphate buffer solution (50 mM, pH 2.5) containing 15 mM HP--CD as a chiral selector. The recommended applied voltage, capillary temperature, and injection time were 15 kV, 25C, and 6 s, respectively. Within-day and interday reproducibility of peak area and migration time gave relative standard deviation values ranging from 1.053.30. Good recoveries (range of 96.8104.9) were obtained from the determination of placebos that were spiked with 0.251.00 mg/L primaquine. The proposed CE method was successfully applied to the assay of primaquine diphosphate in pharmaceutical formulations (tablets).

A Charge Coupled Device Array Detector for Single-Wavelength and Multiwavelength Ultraviolet Absorbance in Capillary Electrophoresis

1999 ◽  
Vol 71 (19) ◽  
pp. 4376-4384 ◽  
Author(s):  
Edmund T. Bergström ◽  
David M. Goodall ◽  
Boris Pokrić ◽  
Nigel M. Allinson
Keyword(s):  
Capillary Electrophoresis ◽  
Array Detector ◽  
Charge Coupled Device ◽  
Ultraviolet Absorbance ◽  
A Charge

Error Measurement and Calibration in Developing Virtual-Reality-Assisted Microassembly System

2015 ◽  
Vol 9 (6) ◽  
pp. 619-628 ◽  
Author(s):  
Ren-Jung Chang ◽  
◽  
Jia-Cheng Jau
Keyword(s):  
Virtual Reality ◽  
Virtual Environment ◽  
System Development ◽  
Calibration Method ◽  
Development Stage ◽  
Deviation Angle ◽  
Charge Coupled Device ◽  
Virtual System ◽  
Representative Points ◽  
Operational Error

The operational error of a virtual reality (VR) assisted vision-based microassembly system is measured and calibrated during the system development stage. The vision-based microassembly system was designed and its opto-mechanical model was established in a virtual environment. By measuring the errors between the representative points in the images of virtual cameras and charge-coupled device (CCD) visual systems, the errors in the virtual environment were compensated by correcting the projection matrix parameters, the view matrix parameters, the initial components position, and the deviation angle for the working stage motion. The effectiveness of the proposed VR-assisted fine calibration method was tested by performing VR calibration on the virtual system.

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