Lipid profile of fish species by liquid chromatography coupled to mass spectrometry and a novel linear retention index database

Suspected tetrodotoxin (TTX) poisoning was associated with eating unknown fish in April 2009 in Taiwan. After ingestion of the fish, symptoms of the victim included perioral paresthesia, nausea, vomiting, ataxia, weakness of all limbs, respiration failure, and death within several hours. The toxicity in the remaining fish was determined, with the mice exhibiting symptoms of neurotoxin poisoning. The implicated fish and deceased victim tissues were analyzed for TTX by liquid chromatography–tandem mass spectrometry. The urine, bile, cerebrospinal fluid (spinal cord), pleural effusion, and pericardial effusion of the victim contained TTX. In addition, the partial cytochrome b gene of the implicated fish was determined by PCR. The DNA sequence in the partial 465-bp cytochrome b gene identified the implicated fish as Chelonodon patoca (puffer fish). These results indicate that people should avoid eating unknown fish species from fish markets where harvested fish may include toxic species.

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Non-psychoactive cannabinoids identification by linear retention index approach applied to a hand-portable capillary liquid chromatography platform

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-021-03871-x ◽

2022 ◽

Author(s):

Roberta La Tella ◽

Francesca Rigano ◽

Paolo Guarnaccia ◽

Paola Dugo ◽

Luigi Mondello

Keyword(s):

Liquid Chromatography ◽

Retention Index ◽

Capillary Liquid Chromatography ◽

Index Approach ◽

Linear Retention Index ◽

Capillary Liquid

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Ciguatoxin Detection in Flesh and Liver of Relevant Fish Species from the Canary Islands

Toxins ◽

10.3390/toxins14010046 ◽

2022 ◽

Vol 14 (1) ◽

pp. 46

Author(s):

María José Ramos-Sosa ◽

Natalia García-Álvarez ◽

Andres Sanchez-Henao ◽

Freddy Silva Silva Sergent ◽

Daniel Padilla ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Canary Islands ◽

Fish Species ◽

Hepatic Toxicity ◽

Liquid Chromatography Mass Spectrometry ◽

Fish Flesh ◽

Dusky Grouper ◽

The Common ◽

First Sale

The Canary Islands are a ciguatoxin (CTX) hotspot with an established official monitoring for the detection of CTX in fish flesh from the authorised points of first sale. Fish caught by recreational fishermen are not officially tested and the consumption of toxic viscera or flesh could lead to ciguatera poisoning (CP). The objectives of this study were to determine the presence of CTX-like toxicity in relevant species from this archipelago, compare CTX levels in liver and flesh and examine possible factors involved in their toxicity. Sixty amberjack (Seriola spp.), 27 dusky grouper (Epinephelus marginatus), 11 black moray eels (Muraena helena) and 11 common two-banded seabream (Diplodus vulgaris) were analysed by cell-based assay (CBA) and Caribbean ciguatoxin-1 (C-CTX1) was detected by liquid chromatography mass spectrometry (LC-MS/MS) in all these species. Most of the liver displayed higher CTX levels than flesh and even individuals without detectable CTX in flesh exhibited hepatic toxicity. Black moray eels stand out for the large difference between CTX concentration in both tissues. None of the specimens with non-toxic liver showed toxicity in flesh. This is the first evidence of the presence of C-CTX1 in the common two-banded seabream and the first report of toxicity comparison between liver and muscle from relevant fish species captured in the Canary Islands.

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