Rhinovirus infection-induced alteration of tight junction and adherens junction components in human nasal epithelial cells

2009 ◽  
Vol 120 (2) ◽  
pp. 346-352 ◽  
Author(s):  
Nam-Kyung Yeo ◽  
Yong Ju Jang
Keyword(s):  
Epithelial Cells ◽  
Tight Junction ◽  
Adherens Junction ◽  
Nasal Epithelial Cells ◽  
Rhinovirus Infection ◽  
Human Nasal Epithelial Cells

Thymic stromal lymphopoietin enhances tight-junction barrier function of human nasal epithelial cells

2009 ◽  
Vol 338 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Ryuta Kamekura ◽  
Takashi Kojima ◽  
Jun-ichi Koizumi ◽  
Noriko Ogasawara ◽  
Makoto Kurose ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tight Junction ◽  
Barrier Function ◽  
Thymic Stromal Lymphopoietin ◽  
Nasal Epithelial Cells ◽  
Human Nasal Epithelial Cells

Protein Kinase C Enhances Tight Junction Barrier Function of Human Nasal Epithelial Cells in Primary Culture by Transcriptional Regulation

2008 ◽  
Vol 74 (2) ◽  
pp. 432-442 ◽  
Author(s):  
Jun-ichi Koizumi ◽  
Takashi Kojima ◽  
Noriko Ogasawara ◽  
Ryuta Kamekura ◽  
Makoto Kurose ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Tight Junction ◽  
Primary Culture ◽  
Barrier Function ◽  
Nasal Epithelial Cells ◽  
Kinase C ◽  
Human Nasal Epithelial Cells

scholarly journals Diesel exposure increases susceptibility of primary human nasal epithelial cells to rhinovirus infection

2021 ◽  
Vol 9 (18) ◽  
Author(s):  
Loretta Müller ◽  
Jakob Usemann ◽  
Marco P. Alves ◽  
Philipp Latzin
Keyword(s):  
Epithelial Cells ◽  
Nasal Epithelial Cells ◽  
Rhinovirus Infection ◽  
Human Nasal Epithelial Cells

scholarly journals Berberine and Obatoclax Inhibit SARS-Cov-2 Replication in Primary Human Nasal Epithelial Cells In Vitro

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 282
Author(s):  
Finny S. Varghese ◽  
Esther van Woudenbergh ◽  
Gijs J. Overheul ◽  
Marc J. Eleveld ◽  
Lisa Kurver ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Early Stage ◽  
Antiviral Drugs ◽  
Target Cells ◽  
Nasal Epithelial Cells ◽  
Cytokines And Chemokines ◽  
Human Nasal Epithelial Cells ◽  
Apoptotic Protein ◽  
Air Liquid Interface

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged as a new human pathogen in late 2019 and it has infected over 100 million people in less than a year. There is a clear need for effective antiviral drugs to complement current preventive measures, including vaccines. In this study, we demonstrate that berberine and obatoclax, two broad-spectrum antiviral compounds, are effective against multiple isolates of SARS-CoV-2. Berberine, a plant-derived alkaloid, inhibited SARS-CoV-2 at low micromolar concentrations and obatoclax, which was originally developed as an anti-apoptotic protein antagonist, was effective at sub-micromolar concentrations. Time-of-addition studies indicated that berberine acts on the late stage of the viral life cycle. In agreement, berberine mildly affected viral RNA synthesis, but it strongly reduced infectious viral titers, leading to an increase in the particle-to-pfu ratio. In contrast, obatoclax acted at the early stage of the infection, which is in line with its activity to neutralize the acidic environment in endosomes. We assessed infection of primary human nasal epithelial cells that were cultured on an air-liquid interface and found that SARS-CoV-2 infection induced and repressed expression of specific sets of cytokines and chemokines. Moreover, both obatoclax and berberine inhibited SARS-CoV-2 replication in these primary target cells. We propose berberine and obatoclax as potential antiviral drugs against SARS-CoV-2 that could be considered for further efficacy testing.

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