Ratiometric Fluorescent Ion Detection in Water with High Sensitivity via Aggregation-Mediated Fluorescence Resonance Energy Transfer Using a Conjugated Polyelectrolyte as an Optical Platform

2013 ◽  
Vol 34 (9) ◽  
pp. 772-778 ◽  
Author(s):  
Van Sang Le ◽  
Boram Kim ◽  
Wonho Lee ◽  
Ji-Eun Jeong ◽  
Renqiang Yang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Ion Detection ◽  
Conjugated Polyelectrolyte ◽  
Fluorescence Resonance

scholarly journals Graphdiyne nanosheets as a platform for accurate copper(ii) ion detection via click chemistry and fluorescence resonance energy transfer

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5320-5324
Author(s):  
Chenchen Ge ◽  
Jiaofu Li ◽  
Dou Wang ◽  
Kongpeng Lv ◽  
Quan Liu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Quenching ◽  
Click Chemistry ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Specificity ◽  
Ion Detection ◽  
Fluorescence Resonance

Cu2+ detection was performed by taking advantage of the fluorescence quenching ability of graphdiyne and the high specificity of click chemistry.

Fluorescence Resonance Energy Transfer-Based Wash-Free Bacterial Imaging and Antibacterial Application Using a Cationic Conjugated Polyelectrolyte

2018 ◽  
Vol 10 (33) ◽  
pp. 27603-27611 ◽  
Author(s):  
Nehal Zehra ◽  
Deepanjalee Dutta ◽  
Akhtar Hussain Malik ◽  
Siddhartha Sankar Ghosh ◽  
Parameswar Krishnan Iyer
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Conjugated Polyelectrolyte ◽  
Fluorescence Resonance

scholarly journals An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

Chemosensors ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 309
Author(s):  
Yongning Li ◽  
Zhenfei Peng ◽  
Yaxi Li ◽  
Min Xiao ◽  
Gongjun Tan ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Ochratoxin A ◽  
Fluorescence Resonance Energy Transfer ◽  
Limit Of Detection ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Detection Methods ◽  
Fluorescence Signal ◽  
Fluorescence Resonance

Food toxins are a hidden threat that can cause cancer and tremendously impact human health. Therefore, the detection of food toxins in a timely manner with high sensitivity is of paramount importance for public health and food safety. However, the current detection methods are relatively time-consuming and not practical for field tests. In the present work, we developed a novel aptamer-chip-based sample-to-answer biosensor (ACSB) for ochratoxin A (OTA) detection via fluorescence resonance energy transfer (FRET). In this system, a cyanine 3 (Cy3)-labeled OTA-specific biotinylated aptamer was immobilized on an epoxy-coated chip via streptavidin-biotin binding. A complementary DNA strand to OTA aptamer at the 3′-end was labeled with a black hole quencher 2 (BHQ2) to quench Cy3 fluorescence when in proximity. In the presence of OTA, the Cy3-labeled OTA aptamer bound specifically to OTA and led to the physical separation of Cy3 and BHQ2, which resulted in an increase of fluorescence signal. The limit of detection (LOD) of this ACSB for OTA was 0.005 ng/mL with a linearity range of 0.01–10 ng/mL. The cross-reactivity of ACSB against other mycotoxins, ochratoxin B (OTB), aflatoxin B1 (AFB1), zearalenone (ZEA), or deoxynilvalenol (DON), was less than 0.01%. In addition, this system could accurately detect OTA in rice samples spiked with OTA, and the mean recovery rate of the spiked-in OTA reached 91%, with a coefficient of variation (CV) of 8.57–9.89%. Collectively, the ACSB may represent a rapid, accurate, and easy-to-use platform for OTA detection with high sensitivity and specificity.

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