Micro‐XRD and nanoindentation investigation of bioceramics for dental pulp therapy

Diagnosis of pulp status is an important clinical step to achieve success in pulp therapy technique or endodontic treatment in children. In pediatric dentistry, history of symptoms given by a child may not be reliable. Assessment of dental pulp status plays an important role. It is hoped that these guidelines will facilitate pulpal diagnosis, good decision-making and evidence-based practice for pediatric patients.DOI: http://doi.dx.org/10.3329/bjdre.v2i2.16248 Bangladesh Journal of Dental Research & Education Vol.2(2) 2012: 66-68

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Dental pulp therapy advances

Dental Abstracts ◽

10.1016/j.denabs.2019.05.053 ◽

2019 ◽

Vol 64 (5) ◽

pp. 355-357

Keyword(s):

Dental Pulp ◽

Pulp Therapy

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Current and future options for dental pulp therapy

Japanese Dental Science Review ◽

10.1016/j.jdsr.2018.09.001 ◽

2019 ◽

Vol 55 (1) ◽

pp. 5-11 ◽

Cited By ~ 15

Author(s):

Takahiko Morotomi ◽

Ayako Washio ◽

Chiaki Kitamura

Keyword(s):

Dental Pulp ◽

Pulp Therapy

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Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (Eugenol) on Inflammatory and Apoptosis Processes in Dental Pulp Fibroblasts

Mediators of Inflammation ◽

10.1155/2016/9371403 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Andrea Martínez-Herrera ◽

Amaury Pozos-Guillén ◽

Socorro Ruiz-Rodríguez ◽

Arturo Garrocho-Rangel ◽

Antonio Vértiz-Hernández ◽

...

Keyword(s):

Dental Pulp ◽

Primary Teeth ◽

Third Molars ◽

Pulp Therapy ◽

Inflammatory Agent ◽

Zinc Oxide Powder ◽

Pulp Inflammation ◽

Apoptotic Genes ◽

Negative Controls ◽

Direct Capping

Eugenol (mixed with zinc oxide powder) is widely used as direct capping material during pulp therapy in primary teeth. The aim of the present study was to evaluate the effect of eugenol on diverse genes involved in inflammatory and cell apoptosis processes. The regulatory effect of eugenol on the expression of inflammation and apoptotic genes was evaluated in dental pulp fibroblasts from extracted third molars, cultured under concentration of eugenol of 13 μM. Eugenol allowed the expression of inflammatory and apoptotic genes when compared with positive and negative controls. Eugenol is a proinflammatory agent when it is in direct contact with healthy tissues and behaves as an anti-inflammatory agent in tissues undergoing inflammatory/apoptotic processes, as in cases of pulp inflammation in primary teeth. These findings are relevant for dentistry, when considering the application of safer pulp treatments to grossly carious children’s teeth.

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Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study

BMC Oral Health ◽

10.1186/s12903-021-01979-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Sedigheh Khedmat ◽

Pegah Sarraf ◽

Ehsan Seyedjafari ◽

Parisa Sanaei-rad ◽

Faranak Noori

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Periodontal Ligament ◽

In Vitro Study ◽

Vitro Study ◽

Control Group ◽

Periodontal Ligament Fibroblasts ◽

Pulp Therapy ◽

Vital Pulp Therapy

Abstract Background Biocompatibility and induction of mineralized tissue formation are the properties expected from a material used in vital pulp therapy and repair of perforations. Cold ceramic (SJM, Iran; CC) is a newly introduced calcium silicate-based cement for above mentioned therapeutic applications. This in-vitro study aimed to compare the effect of CC and White MTA-Angelus (MTA) on cell viability, attachment, odontogenic differentiation, and calcification potential of human dental pulp stem cells (DPSCs) and periodontal ligament fibroblasts (PDLFs). Methods Cell viability of DPSCs and PDLFs was assessed using MTT on days 1, 3, 7, and 14 (n = 9) in contact with freshly mixed and set states of CC and MTA. Field emission scanning electron micrographs (FESEM) were taken to evaluate cell-bioceramic interaction (n = 6). Gene expression levels of osteo/odontogenic markers (Dentin sialophosphoprotein, Dentin matrix protein 1, Collagen type I alpha 1, and Alkaline phosphatase (DSPP, DMP1, COL 1A1, and ALP, respectively) (n = 8) were assessed using qrt-PCR. ALP enzymatic activity was evaluated to assess the mineralization potential. A two-way ANOVA test was applied, and p < 0.05 was considered to be statistically significant. Results The effect of freshly mixed and set MTA and CC on the survival of DPSCs and PDLFs in all study groups was statistically similar and comparable to the positive control group (p > 0.05); the only exception was for the viability of PDLFs in contact with freshly mixed cements on day 1, showing a more significant cytotoxic effect compared to the control and the set state of materials (p < 0.05). PDLFs attached well on CC and MTA. The spread and pseudopodium formation of the cells increased on both samples from day 1 to day 14. Contact of MTA and CC with DPSCs similarly increased expression of all dentinogenesis markers studied on days 7 and 14 compared to the control group (p < 0.001), except for DSPP expression on day 7 (p = 0.46 and p = 0.99 for MTA and CC, respectively). Conclusions Within the limitation of this in-vitro study, cold ceramic and MTA-Angelus showed high biocompatibility and induced increased expression of osteo/dentinogenic markers. Therefore, cold ceramic can be a suitable material for vital pulp therapy and the repair of root perforations.

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LECZENIE BIOLOGICZNE MIAZGI ZĘBÓW STAŁYCH

Pomeranian Journal of Life Sciences ◽

10.21164/pomjlifesci.38 ◽

2016 ◽

Vol 60 (2) ◽

Author(s):

Piotr Makowiecki ◽

Matylda Trusewicz ◽

Łukasz Tyszler ◽

Jadwiga Buczkowska-Radlińska

Keyword(s):

Treatment Option ◽

Dental Pulp ◽

Permanent Teeth ◽

Accurate Assessment ◽

Tissue Destruction ◽

Pulp Tissue ◽

Pulp Therapy ◽

Vital Pulp Therapy ◽

Tooth Survival ◽

Tooth Tissue

The vitality of dental pulp is essential for long‍‑term tooth survival. The aim of vital pulp therapy is to preserve vital, healthy pulp tissue. This therapy’s foundation is the elimination of bacteria from the dentin‍‑pulp complex. The treatment option depends on the cause and extent of mineralised tooth tissue destruction. The outcome of such treatment is determined by accurate assessment of the pulp’s status and the dentist’s ability to predict the success of the therapy. The aim of this review is to facilitate the dentist in making a proper decision referring to vital pulp therapy in permanent teeth, and to provide an overview of new approaches in such treatment.

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Dental Pulp Stem Cells from Primary and Permanent Teeth: Quality Analysis

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.35.1.e33x0135r7670287 ◽

2010 ◽

Vol 35 (1) ◽

pp. 53-58 ◽

Cited By ~ 6

Author(s):

Shobha Tandon ◽

Rooposhi Saha ◽

Ramesh Rajendran ◽

Rashmi Nayak

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Dental Pulp ◽

Adult Stem Cells ◽

Quality Analysis ◽

Permanent Teeth ◽

Surface Markers ◽

Differentiation Potential ◽

Pulp Therapy ◽

Significant Difference

Objective: The objective of the study was to identify and isolate stem cells from healthy and inflamed dental pulp and characterize their differentiation potential into multiple lineages. Study design: Study was conducted in dental pulp tissues obtained from the children in the age range of 5-14 years. Tissue samples were collected from teeth indicated for pulp therapy and extractions for orthodontic purpose. Samples were processed in the laboratory including cell culture, isolation and differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow which is considered as a gold standard. Results: There was no statistically significant difference found in the expression of various surface markers between inflamed dental pulp and bone marrow. Healthy pulp from the primary teeth was not sufficient to use as a source for harvesting stem cells moreover the healthy tissue obtained from permanent teeth failed to show any results at all. Conclusion: Inflamed pulp discarded during pulp therapy procedures is a potential source for harvesting adult stem cells.

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Vital Pulp Therapy—Current Progress of Dental Pulp Regeneration and Revascularization

International Journal of Dentistry ◽

10.1155/2010/856087 ◽

2010 ◽

Vol 2010 ◽

pp. 1-9 ◽

Cited By ~ 45

Author(s):

Weibo Zhang ◽

Pamela C. Yelick

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

The Body ◽

Void Space ◽

Synthetic Material ◽

Pulp Tissue ◽

Pulp Regeneration ◽

Pulp Therapy ◽

Vital Pulp Therapy ◽

Review Current

Pulp vitality is extremely important for the tooth viability, since it provides nutrition and acts as biosensor to detect pathogenic stimuli. In the dental clinic, most dental pulp infections are irreversible due to its anatomical position and organization. It is difficult for the body to eliminate the infection, which subsequently persists and worsens. The widely used strategy currently in the clinic is to partly or fully remove the contaminated pulp tissue, and fill and seal the void space with synthetic material. Over time, the pulpless tooth, now lacking proper blood supply and nervous system, becomes more vulnerable to injury. Recently, potential for successful pulp regeneration and revascularization therapies is increasing due to accumulated knowledge of stem cells, especially dental pulp stem cells. This paper will review current progress and feasible strategies for dental pulp regeneration and revascularization.

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