Steroidogenesis by isolated porcine oocyte-cumulus complexes: Lack of an inhibitory effect of low molecular weight fraction of porcine follicular fluid on conversion of androgen to estrogen

1982 ◽  
Vol 5 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Frederick W. Schaerf ◽  
Larry D. Anderson ◽  
Cornelia P. Channing
Keyword(s):  
Molecular Weight ◽  
Follicular Fluid ◽  
Weight Fraction ◽  
Inhibitory Effect ◽  
Low Molecular Weight ◽  
Porcine Oocyte

In Vivo Studies on the Inhibition of Coagulation by Fractionated Heparin and by a Heparin Analogue I. Effects of Heparin Fractions

1981 ◽  
Vol 46 (03) ◽  
pp. 612-616 ◽  
Author(s):  
U Schmitz-Huebner ◽  
L Balleisen ◽  
F Asbeck ◽  
J van de Loo
Keyword(s):  
Molecular Weight ◽  
Day Treatment ◽  
Gel Filtration ◽  
Weight Fraction ◽  
In Vivo Studies ◽  
Low Molecular Weight ◽  
High Molecular Weight Fraction ◽  
Platelet Factor ◽  
Heparin Fractions

SummaryHigh and low molecular weight heparin fractions obtained by gel filtration chromatography of sodium mucosal heparin were injected subcutaneously into six healthy volunteers and compared with the unfractionated substance in a cross-over trial. Equal doses of 5,000 U were administered twice daily over a period of three days and heparin activity was repeatedly controlled before and 2, 4, 8 hrs after injection by means of the APTT, the anti-Xa clotting test and a chromogenic substrate assay. In addition, the in vivo effect of subcutaneously administered fractionated heparin on platelet function was examined on three of the volunteers. The results show that s.c. injections of the low molecular weight fraction induced markedly higher anti-Xa activity than injections of the other preparations. At the same time, APTT results did not significantly differ. Unfractionated heparin and the high molecular weight fraction enhanced ADP-induced platelet aggregation and collagen-mediated MDA production, while the low molecular weight fraction hardly affected these assays, but potently inhibited thrombin-induced MDA production. All heparin preparations stimulated the release of platelet Factor 4 in plasma. During the three-day treatment periods, no side-effects and no significant changes in the response to heparin injections were detected.

scholarly journals Inhibitory effect of low-molecular-weight peptides (0–3 kDa) from Spirulina platensis on H2O2-induced oxidative damage in L02 human liver cells

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jun Ma ◽  
Xiankun Zeng ◽  
Min Zhou ◽  
Le Cheng ◽  
Difeng Ren
Keyword(s):  
Molecular Weight ◽  
Oxidative Damage ◽  
Spirulina Platensis ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Control Group ◽  
Low Molecular Weight ◽  
Sod Activity ◽  
Liver Health ◽  
L02 Cells

AbstractSpirulina platensis protein hydrolysates were prepared by digesting protein extracts with papain, and the hydrolysates were separated into 30, 10, and 3 kDa weights using membrane ultrafiltration. The 0–3 kDa low-molecular-weight Spirulina peptides (LMWSPs) proved the highest chemical antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, hydroxyl radical (·OH) scavenging activities and total antioxidant capacity. Cellular antioxidant ability of LMWPs fractions against 2000 μg/mL H2O2 induced oxidative damage of L02 cells were investigated. The MTT assay results displayed that LMWSPs at different concentrations (0–1000 μg/mL) had proliferation effect on the L02 cells and that treatment of the L02 cells with the 1000 μg/mL LMWSPs (0–3 kDa) significantly prevented H2O2-induced oxidative damage compared with control cells. Moreover, the 2′,7′-dichlorofluorescein diacetate (DCFH-DA) fluorescent probe assay showed that the levels of ROS and NO were significantly lower in the experimental group that was treated with the peptides for 24 h than in the control group. Furthermore, using the corresponding kits, the treatment inhibited the reduction of SOD activity and the increase of MDA contents in the L02 cells. Therefore, LMWSPs (0–3 kDa) may have potential applications in antioxidant and liver health products.

scholarly journals Candidate markers for the detection of hepatocellular carcinoma in low-molecular weight fraction of serum

2007 ◽  
Vol 28 (10) ◽  
pp. 2149-2153 ◽  
Author(s):  
Radoslav Goldman ◽  
Habtom W. Ressom ◽  
Mohamed Abdel-Hamid ◽  
Lenka Goldman ◽  
Antai Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Molecular Weight ◽  
Weight Fraction ◽  
Low Molecular Weight

Anti-Inflammatory Activity in the Low Molecular Weight Fraction of Commercial Human Serum Albumin (LMWF5A)

2015 ◽  
Vol 37 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Gregory W. Thomas ◽  
Leonard T. Rael ◽  
Charles W. Mains ◽  
Denetta Slone ◽  
Matthew M. Carrick ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Weight Fraction ◽  
Inflammatory Activity ◽  
Low Molecular Weight ◽  
Anti Inflammatory

scholarly journals Effect of kinetin on nucleic acid synthesis in senescing and young leaves in Brassica oleracea L. var. gongylodes L.

2015 ◽  
Vol 44 (4) ◽  
pp. 553-565
Author(s):  
J. Legocka ◽  
A. Szweykowska
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Acid Synthesis ◽  
Inhibitory Effect ◽  
Rrna Synthesis ◽  
Low Molecular Weight ◽  
Chlorophyll B ◽  
Mature Leaves ◽  
Young Leaves ◽  
Brassica Oleracea L

In detached kohlrabi leaves senescing in the dark, the decrease in chlorophyll to was more pronounced than in chlorophyll a. The retardation by kinetin of the chlorophyll loss was also markedly stronger in the case of chlorophyll b. Using the fractionation of nucleic acids on polyacrylamide gels it has been shown that during leaf senescence the level of all RNA species decreased, whereas the amount of DNA was more or less constant. In the presence of kinetin, the loss of RNA was inhibited and the incorporation of precursor into the cytoplasmic rRNA as well as into low molecular weight RNA species was supported. Chloroplast rRNA synthesis has not been detected in mature leaves and kinetin showed no effect in this respect. In young expanding leaves detached and kept in light, the synthesis of cytoplasmic rRNA was strongly stimulated by kinetin, whereas in the case of Chloroplast rRNA only an inhibitory effect of kinetin could be found. The results suggest that the cytokinins are primarily involved in processes of the synthesis of cytoplasmic rRNA and low molecular RNA fractions, and in this way affect the development of plastids, in particular the course of their senescence.

Characterization of Sol and Gel in Hevea Natural Rubber

1997 ◽  
Vol 70 (5) ◽  
pp. 707-713 ◽  
Author(s):  
Jitladda Tangpakdee ◽  
Yasukuki Tanaka
Keyword(s):  
Molecular Weight ◽  
Weight Fraction ◽  
Toluene Solution ◽  
Low Molecular Weight ◽  
Branch Points ◽  
Gel Content ◽  
High Ammonia ◽  
Linear Molecules ◽  
Cis And Trans

Abstract The gel content of rubber from high-ammonia latex (HA-latex) decreased significantly after deproteinization with proteolytic enzyme. The addition of 1–2% ethanol in toluene solution reduced the gel content of rubbers from HA-latex, deproteinized HA-latex (HA-DP) and pale crepe. Transesterification of the rubber in toluene solution with sodium methoxide dissolved the gel fraction. The gel fractions solubilized after transesterification showed molecular weight distribution rich in low molecular-weight fraction. The Huggins k′ constant of the fractionated rubbers from solubilized-gels was in the range of 0.42–0.45, lower than that of the fractionated HA-DP of 0.5–0.8. This indicates that all the branch-points were decomposed by transesterification to form linear molecules. The Mn values of rubber chains assembling the gel was 5.5−8.3×105 by 13C-NMR measurements of the ratio between cis- and trans-isoprene units, which were comparable to the molecular weight between crosslinks, Mc, of 7−11×105 by swelling measurements. These findings suggest that the branching and crosslinks are composed of two types of branch-points, i. e. one by association or aggregation of proteins or oligopeptides at the initiating end and the other by ester linkages including phosphoric ester at the terminal end.

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