A comparative study of short- and long-TE 1
H MRS at 3 T for in vivo
 detection of 2-hydroxyglutarate in brain tumors

SummaryThe distribution of 32P-polyphosphates (lineal and cross-linked) and 32Porthophosphate in normal and tumor bearing animals has been studied. Differences between the cross-linked and the lineal form are related to a different degree of susceptibility to the hydrolysis by the phosphatases. In contrast to orthophosphate, the polyphosphates showed a lower accumulation in soft tissues which gives an advantageous reduction of the total body radiation dose.

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Effects of a Monoclonal Antibody to Glycoprotein IIb/IIIa (P256) and of Enzymically Derived Fragments of P256 on Human Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648166 ◽

1991 ◽

Vol 65 (04) ◽

pp. 432-437 ◽

Cited By ~ 9

Author(s):

A W J Stuttle ◽

M J Powling ◽

J M Ritter ◽

R M Hardisty

Keyword(s):

Monoclonal Antibody ◽

Flow Cytometry ◽

Platelet Aggregation ◽

Calcium Ions ◽

Human Platelet ◽

Human Platelets ◽

In Vivo Detection ◽

Fibrinogen Binding ◽

Specific Antagonist

SummaryThe anti-platelet monoclonal antibody P256 is currently undergoing development for in vivo detection of thrombus. We have examined the actions of P256 and two fragments on human platelet function. P256, and its divalent fragment, caused aggregation at concentrations of 10−9−3 × 10−8 M. A monovalent fragment of P256 did not cause aggregation at concentrations up to 10−7 M. P256–induced platelet aggregation was dependent upon extracellular calcium ions as assessed by quin2 fluorescence. Indomethacin partially inhibited platelet aggregation and completely inhibited intracellular calcium mobilisation. Apyrase caused partial inhibition of aggregation. Aggregation induced by the divalent fragment was dependent upon fibrinogen and was inhibited by prostacyclin. Aggregation induced by the whole antibody was only partially dependent upon fibrinogen, but was also inhibited by prostacyclin. P256 whole antibody was shown, by flow cytometry, to induce fibrinogen binding to indomethacin treated platelets. Monovalent P256 was shown to be a specific antagonist for aggregation induced by the divalent forms. In–111–labelled monovalent fragment bound to gel-filtered platelets in a saturable and displaceable manner. Monovalent P256 represents a safer form for in vivo applications

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Faculty Opinions recommendation of In vivo comparative study of RNAi methodologies by in ovo electroporation in the chick embryo.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1021783.247701 ◽

2004 ◽

Author(s):

Harukazu Nakamura

Keyword(s):

Chick Embryo ◽

Comparative Study ◽

In Ovo ◽

In Ovo Electroporation

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Faculty Opinions recommendation of Fluorescent in vivo detection reveals that IgE(+) B cells are restrained by an intrinsic cell fate predisposition.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.14257003.15768150 ◽

2012 ◽

Author(s):

Dan Conrad

Keyword(s):

B Cells ◽

Cell Fate ◽

In Vivo Detection

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Faculty Opinions recommendation of Rapid in vivo detection of isoniazid-sensitive Mycobacterium tuberculosis by breath test.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718885651.793501966 ◽

2014 ◽

Author(s):

Vojo Deretic

Keyword(s):

Mycobacterium Tuberculosis ◽

Breath Test ◽

In Vivo Detection

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A Mouse Model for in Vivo Detection and Disruption of TGF-Beta Signaling in Breast Cancer Metastasis

10.21236/ada512330 ◽

2009 ◽

Author(s):

Manav Korpal

Keyword(s):

Breast Cancer ◽

Mouse Model ◽

Cancer Metastasis ◽

Breast Cancer Metastasis ◽

Tgf Beta ◽

In Vivo Detection

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Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

Current Analytical Chemistry ◽

10.2174/1573411014666180510145320 ◽

2019 ◽

Vol 15 (5) ◽

pp. 567-574

Author(s):

Huck Jun Hong ◽

Suw Young Ly

Keyword(s):

Carbon Nanotube ◽

Real Time ◽

Anticancer Agents ◽

Cancer Metastasis ◽

Anodic Stripping Voltammetry ◽

Stripping Voltammetry ◽

Takifugu Rubripes ◽

In Vivo Detection ◽

Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

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