Inorganic nuclear content as an index of tumour radiosensitivity

1939 ◽  
Vol 48 (1) ◽  
pp. 1-10 ◽  
Author(s):  
I. A. B. Cathie
Keyword(s):  
Nuclear Content

Changes in nuclear content of protein conjugate histone H2A-ubiquitin during rooster spermatogenesis

FEBS Letters ◽  
1983 ◽  
Vol 155 (2) ◽  
pp. 209-212 ◽  
Author(s):  
Neus Agell ◽  
Manel Chiva ◽  
Cristóbal Mezquita
Keyword(s):  
Histone H2a ◽  
Protein Conjugate ◽  
Nuclear Content

scholarly journals Mechanisms of nuclear content loading to exosomes

2019 ◽  
Vol 5 (11) ◽  
pp. eaax8849 ◽  
Author(s):  
Akira Yokoi ◽  
Alejandro Villar-Prados ◽  
Paul Allen Oliphint ◽  
Jianhua Zhang ◽  
Xingzhi Song ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Small Rnas ◽  
Genomic Dna ◽  
Multivesicular Body ◽  
Imaging Flow Cytometry ◽  
Intracellular Compartments ◽  
The Relationship ◽  
Nuclear Content

Exosome cargoes are highly varied and include proteins, small RNAs, and genomic DNA (gDNA). The presence of gDNA suggests that different intracellular compartments contribute to exosome loading, resulting in distinct exosome subpopulations. However, the loading of gDNA and other nuclear contents into exosomes (nExo) remains poorly understood. Here, we identify the relationship between cancer cell micronuclei (MN), which are markers of genomic instability, and nExo formation. Imaging flow cytometry analyses reveal that 10% of exosomes derived from cancer cells and <1% of exosomes derived from blood and ascites from patients with ovarian cancer carry nuclear contents. Treatment with genotoxic drugs resulted in increased MN and nExos both in vitro and in vivo. We observed that multivesicular body precursors and exosomal markers, such as the tetraspanins, directly interact with MN. Collectively, this work provides new insights related to nExos, which have implications for cancer biomarker development.

DNA nuclear content in the cytotaxonomy of Galago senegalensis and Galago crassicaudatus

1972 ◽  
Vol 1 (5) ◽  
pp. 473-476 ◽  
Author(s):  
M.G. Manfredi-Romanini ◽  
L.E.M. de Boer ◽  
B. Chiarelli ◽  
S. Tinozzi Massari
Keyword(s):  
Galago Crassicaudatus ◽  
Galago Senegalensis ◽  
Nuclear Content

scholarly journals Genome size in Tribolium flour-beetles: inter- and intraspecific variation

1991 ◽  
Vol 58 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Asunción Alvarez-Fuster ◽  
Carlos Juan ◽  
Eduard Petitpierre
Keyword(s):  
Genome Size ◽  
Intraspecific Variation ◽  
Related Species ◽  
Alphitobius Diaperinus ◽  
Flour Beetles ◽  
Nuclear Content

SummaryEight species of Tribolium and the related species Alphitobius diaperinus have been microdensitometrically analysed by measuring the nuclear content (1C value) of their Feuigenstained spermatids. The range of mean genome sizes goes from 0·157 pg in T. audax to 0·388 pg in T. brevicornis, including five significantly different groups of values. Also, in ail but one species of Tribolium significant intraspecific heterogeneity of genome size was found. The resemblances in genome size are not generally correlated with genetic homologies among species, but there is a highly significant correlation between genome size and spermatid size.

scholarly journals Association of nucleoplasmin with transcription products as revealed by immunolocalization in the amphibian oocyte.

1986 ◽  
Vol 103 (3) ◽  
pp. 683-690 ◽  
Author(s):  
N Moreau ◽  
N Angelier ◽  
M L Bonnanfant-Jais ◽  
P Gounon ◽  
P Kubisz
Keyword(s):  
Transcriptional Activity ◽  
Light And Electron Microscopy ◽  
Nuclear Proteins ◽  
Oocyte Nucleus ◽  
Actin Network ◽  
Previously Treated ◽  
Pleurodeles Waltlii ◽  
Chromosome Axis ◽  
Lampbrush Loops ◽  
Nuclear Content

The oocyte nucleus of Pleurodeles waltlii contains a major 32,000-mol-wt acidic protein which is called nucleoplasmin. Rabbit antibodies were raised against total nuclear proteins from Pleurodeles oocytes. Affinity-purified antibodies directed against nucleoplasmin were prepared using antigens bound to nitrocellulose paper. The specificity of the antibody was controlled on two-dimensional electrophoretic gels of nuclear proteins. The intranuclear distribution of nucleoplasmin was analyzed by indirect immunofluorescence and the immunogold technique in light and electron microscopy. The antibody was tested on a spread of the nuclear content prepared in the presence of calcium, on the nuclear content spread in the presence of phalloidin so that an actin network appeared, and on a spread of nuclei from oocytes previously treated by actinomycin D. In all cases, nucleoplasmin appeared to be localized on the lampbrush loops, i.e., on the sites of transcription and, more specifically, on the ribonucleoprotein (RNP) particles; this protein was also associated with the RNP particles of the nuclear sap (free or inserted in the actin network). Nucleoplasmin was localized on large RNP particles that appeared when transcription was blocked. We never found this protein on the chromosome axis. These results suggest that nucleoplasmin may play a role in transcriptional activity.

Intranuclear distribution of androgen receptors in human prostate carcinoma

1987 ◽  
Vol 112 (1) ◽  
pp. 161-169 ◽  
Author(s):  
N. Kyprianou ◽  
J. C. Gingell ◽  
P. Davies
Keyword(s):  
Prostate Carcinoma ◽  
Androgen Receptors ◽  
Disease Process ◽  
Micrococcal Nuclease ◽  
Human Prostate ◽  
Human Prostate Carcinoma ◽  
High Concentrations ◽  
Prostate Carcinomas ◽  
Nuclear Content

ABSTRACT Androgen receptors in nuclei from human prostate carcinomas were characterized on the basis of their solubilization by, or resistance to, micrococcal nuclease. By this means, androgen receptors were assigned to three nuclear categories: those associated with nuclease-resistant structures, those associated with chromatin and those apparently uncommitted by association with either of these. Prostate carcinoma nuclei contained high concentrations (57–82% of total nuclear content) of nuclease-resistant androgen receptors. This was a different pattern from that observed previously for benign hypertrophic prostate epithelial nuclei which contained a variable high proportion of uncommitted androgen receptors. The differences could not be attributed to differential losses to cytosol, or to loss of functionality, as determined in vitro. The differences in distribution could reflect different responses of diseased cells to androgens, or the intervention of other factors more relevant to the disease process. J. Endocr. (1987) 112, 161–169

Hepatoblastoma: DNA nuclear content, proliferative indices, and pathology

2008 ◽  
Vol 18 (2) ◽  
pp. 128-133 ◽  
Author(s):  
Massimo Rugge ◽  
Fulvia Sonego ◽  
Lucio Pollice ◽  
Giorgio Perilongo ◽  
Maria Guido ◽  
...  
Keyword(s):  
Proliferative Indices ◽  
Nuclear Content
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