Highly sensitive assessment of neuroblastoma minimal residual disease in ovarian tissue using RT-qPCR-A strategy for improving the safety of fertility restoration

2016 ◽  
Vol 64 (5) ◽  
pp. e26287 ◽  
Author(s):  
Victoria Grèze ◽  
Florence Brugnon ◽  
Fanny Chambon ◽  
Pascale Halle ◽  
Michel Canis ◽  
...  
Keyword(s):  
Minimal Residual Disease ◽  
Residual Disease ◽  
Fertility Restoration ◽  
Ovarian Tissue ◽  
Highly Sensitive ◽  
Minimal Residual

scholarly journals Sensitive and Specific Detection of Ewing Sarcoma Minimal Residual Disease in Ovarian and Testicular Tissues in an In Vitro Model

Cancers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1807
Author(s):  
Laure Chaput ◽  
Victoria Grèze ◽  
Pascale Halle ◽  
Nina Radosevic-Robin ◽  
Bruno Pereira ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Minimal Residual Disease ◽  
Ewing Sarcoma ◽  
Residual Disease ◽  
Fertility Restoration ◽  
Es Cells ◽  
Ovarian Tissue ◽  
Culture Techniques ◽  
Minimal Residual

Ewing sarcoma (EWS) is a common pediatric solid tumor with high metastatic potential. Due to toxic effects of treatments on reproductive functions, the cryopreservation of ovarian tissue (OT) or testicular tissue (TT) is recommended to preserve fertility. However, the risk of reintroducing residual metastatic tumor cells should be evaluated before fertility restoration. Our goal was to validate a sensitive and specific approach for EWS minimal residual disease (MRD) detection in frozen germinal tissues. Thawed OT (n = 12) and TT (n = 14) were contaminated with tumor RD-ES cells (10, 100, and 1000 cells) and EWS-FLI1 tumor-specific transcript was quantified with RT-qPCR. All contaminated samples were found to be positive, with a strong correlation between RD-ES cell numbers and EWS-FLI1 levels in OT (r = 0.93) and TT (r = 0.96) (p < 0.001). No transcript was detected in uncontaminated control samples. The invasive potential of Ewing cells was evaluated using co-culture techniques. After co-culturing, tumor cells were detected in OT/TT with histology, FISH, and RT-qPCR. In addition, four OT and four TT samples from children with metastatic EWS were tested, and no MRD was found using RT-qPCR and histology. We demonstrated the high sensitivity and specificity of RT-qPCR to detect EWS MRD in OT/TT samples. Clinical trial: NCT 02400970.

Highly Sensitive Minimal Residual Disease Detection by Biomimetic Multivalent Aptamer Nanoclimber Functionalized Microfluidic Chip

Small ◽  
2020 ◽  
Vol 16 (20) ◽  
pp. 2000949 ◽  
Author(s):  
Yilong Liu ◽  
Huimin Zhang ◽  
Yahui Du ◽  
Zhi Zhu ◽  
Mingxia Zhang ◽  
...  
Keyword(s):  
Minimal Residual Disease ◽  
Microfluidic Chip ◽  
Residual Disease ◽  
Disease Detection ◽  
Minimal Residual Disease Detection ◽  
Highly Sensitive ◽  
Minimal Residual

The MRD disk: automated minimal residual disease monitoring by highly sensitive centrifugal microfluidic multiplex qPCR

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Peter Juelg ◽  
Elena Kipf ◽  
Mara Specht ◽  
Marion Fillies ◽  
Cornelia Eckert ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukaemia ◽  
Minimal Residual Disease ◽  
Lymphoblastic Leukaemia ◽  
Residual Disease ◽  
Microfluidic System ◽  
Disease Monitoring ◽  
Multiplex Qpcr ◽  
Minimal Residual Disease Monitoring ◽  
Highly Sensitive ◽  
Minimal Residual

A centrifugal microfluidic system for automated, highly sensitive and multiplexed qPCR analyses, demonstrated for minimal residual disease monitoring in acute lymphoblastic leukaemia.

Minimal Residual Disease Quantification in Ovarian Tissue Collected in Patients in Complete Remission of Acute Leukemia

Blood ◽  
2020 ◽  
Author(s):  
Florian Chevillon ◽  
Emmanuelle Clappier ◽  
Chloe Arfeuille ◽  
Jean-Michel Cayuela ◽  
Jean-Hugues Dalle ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Complete Remission ◽  
Acute Leukemia ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Ovarian Tissue ◽  
Ovarian Tissue Cryopreservation ◽  
Hematopoietic Stem ◽  
Leukemic Infiltration ◽  
Minimal Residual

Ovarian tissue cryopreservation (OTC) is offered to women treated for acute leukemia to preserve their fertility before hematopoietic stem cell transplantation. The risk of leukemic infiltration in ovarian samples harvested before administration of chemotherapy limits ovarian tissue transplantations. We assessed the minimal residual disease (MRD) by sensitive quantitative polymerase chain reaction in cryopreserved ovarian cortex and medulla samples harvested from 30 patients in complete remission of acute leukemia, including 60 % with negative bone marrow MRD at the time of OTC. Ovarian MRD was undetectable in 21 patients (70%), detectable below 10-4 in 8 patients (27%) and between 10-3 and 10-4 in 1 patient (3%). Twenty patients (67%) had concordant MRD between bone marrow and ovarian samples. Interestingly 4 patients had positive MRD in ovarian samples while undetectable in bone marrow. Our results underline the importance of reaching the best control of the disease with undetectable or low MRD levels before OTC to minimize the risk of ovarian leukemic infiltration. The discordant results between ovarian samples and bone marrow require to test the more ovarian samples available before considering ovarian tissue transplantation.

scholarly journals Harmonization of Flow Cytometric Minimal Residual Disease Assessment in Multiple Myeloma in Centers of Polish Myeloma Consortium

Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1872
Author(s):  
Agnieszka Krzywdzińska ◽  
Bartosz Puła ◽  
Anna Czyż ◽  
Beata Krzymieniewska ◽  
Jolanta Kiernicka-Parulska ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Clinical Trials ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
High Sensitivity ◽  
Disease Assessment ◽  
Reproducibility Study ◽  
Highly Sensitive ◽  
Data Files ◽  
Minimal Residual

Minimal residual disease (MRD) status is now considered as one of the most relevant prognostic factors in multiple myeloma (MM) while MRD negativity became an important endpoint in clinical trials. Here, we report the results of the first study evaluating the reproducibility of high-sensitivity flow cytometry MM MRD assessment in four laboratories in Poland. EuroFlow protocols for instrument setting standardization and sample preparation in MM MRD assessment were implemented in each laboratory. In the inter-laboratory reproducibility study, 12 bone marrow samples from MM patients were distributed and processed in participant laboratories. In the inter-operator concordance study, 13 raw data files from MM MRD measurements were analyzed by five independent operators. The inter-laboratory study showed high 95% overall concordance of results among laboratories. In the inter-operator study, 89% of MRD results reported were concordant, and the highest immunophenotype interpretation differences with regard to expression of CD27, CD45, CD81 were noticed. We confirmed the applicability and feasibility of the EuroFlow protocol as a highly sensitive method of MRD evaluation in MM. Results of our inter-center comparison study demonstrate that the standardization of MM MRD assessment protocols is highly desirable to improve quality and comparability of results within and between different clinical trials.

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