Differential phosphorylation of thylakoid proteins in mesophyll and bundle sheath chloroplasts from maize plants grown under low or high light

PROTEOMICS ◽  
2012 ◽  
Vol 12 (18) ◽  
pp. 2852-2861 ◽  
Author(s):  
Rikard Fristedt ◽  
Wioleta Wasilewska ◽  
Elzbieta Romanowska ◽  
Alexander V. Vener
Keyword(s):  
Bundle Sheath ◽  
High Light ◽  
Thylakoid Proteins ◽  
Maize Plants ◽  
Differential Phosphorylation

scholarly journals The High Light Response in Arabidopsis Involves ABA Signaling between Vascular and Bundle Sheath Cells

2009 ◽  
Vol 21 (7) ◽  
pp. 2143-2162 ◽  
Author(s):  
Gregorio Galvez-Valdivieso ◽  
Michael J. Fryer ◽  
Tracy Lawson ◽  
Katie Slattery ◽  
William Truman ◽  
...  
Keyword(s):  
Light Response ◽  
Bundle Sheath ◽  
High Light ◽  
Aba Signaling ◽  
Bundle Sheath Cells ◽  
Sheath Cells

scholarly journals High Light Acclimation Induces Chloroplast Precursor Phosphorylation and Reduces Import Efficiency

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 24
Author(s):  
Eisa ◽  
Malenica ◽  
Schwenkert ◽  
Bölter
Keyword(s):  
Protein Complexes ◽  
High Light ◽  
Lower Efficiency ◽  
Altered Expression ◽  
Receptor Proteins ◽  
Photosynthetic Machinery ◽  
The One ◽  
Envelope Membranes ◽  
Differential Phosphorylation ◽  
Changing Light

Acclimation is an essential process in plants on many levels, but especially in chloroplasts under changing light conditions. It is partially known how the photosynthetic machinery reacts upon exposure to high light intensities, including rearrangement of numerous protein complexes. Since the majority of proteins residing within chloroplasts needs to be posttranslationally imported into the organelles, we endeavored to study how this important process is regulated upon subjecting plants from pea and Arabidopsis to high light. Our results reveal that acclimation takes place on the one hand in the cytosol by differential phosphorylation of preproteins and resulting from the altered expression of the responsible kinases, and on the other hand at the level of the translocation machineries in the outer (TOC) and inner (TIC) envelope membranes. Intriguingly, while phosphorylation is more pronounced under high light, import itself shows a lower efficiency, along with a reduced accumulation of the Toc receptor proteins Toc34 and Toc159.

scholarly journals Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase

2021 ◽  
Vol 118 (25) ◽  
pp. e2022702118
Author(s):  
Haiyan Xiong ◽  
Lei Hua ◽  
Ivan Reyna-Llorens ◽  
Yi Shi ◽  
Kun-Ming Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Messenger Rna ◽  
Photosynthetic Apparatus ◽  
Reactive Oxygen ◽  
Bundle Sheath ◽  
High Light ◽  
Oxygen Species ◽  
Mesophyll Cells ◽  
Excess Light

When exposed to high light, plants produce reactive oxygen species (ROS). In Arabidopsis thaliana, local stress such as excess heat or light initiates a systemic ROS wave in phloem and xylem cells dependent on NADPH oxidase/respiratory burst oxidase homolog (RBOH) proteins. In the case of excess light, although the initial local accumulation of ROS preferentially takes place in bundle-sheath strands, little is known about how this response takes place. Using rice and the ROS probes diaminobenzidine and 2′,7′-dichlorodihydrofluorescein diacetate, we found that, after exposure to high light, ROS were produced more rapidly in bundle-sheath strands than mesophyll cells. This response was not affected either by CO2 supply or photorespiration. Consistent with these findings, deep sequencing of messenger RNA (mRNA) isolated from mesophyll or bundle-sheath strands indicated balanced accumulation of transcripts encoding all major components of the photosynthetic apparatus. However, transcripts encoding several isoforms of the superoxide/H2O2-producing enzyme NADPH oxidase were more abundant in bundle-sheath strands than mesophyll cells. ROS production in bundle-sheath strands was decreased in mutant alleles of the bundle-sheath strand preferential isoform of OsRBOHA and increased when it was overexpressed. Despite the plethora of pathways able to generate ROS in response to excess light, NADPH oxidase–mediated accumulation of ROS in the rice bundle-sheath strand was detected in etiolated leaves lacking chlorophyll. We conclude that photosynthesis is not necessary for the local ROS response to high light but is in part mediated by NADPH oxidase activity.

scholarly journals Comparative Studies of the Thylakoid Proteins of Mesophyll and Bundle Sheath Plastids of Zea mays

1976 ◽  
Vol 58 (3) ◽  
pp. 345-349 ◽  
Author(s):  
Stefan J. Kirchanski ◽  
Roderic B. Park
Keyword(s):  
Zea Mays ◽  
Comparative Studies ◽  
Bundle Sheath ◽  
Thylakoid Proteins

Sulphate uptake by leaf mesophyll and bundle sheath cells of maize plants

1988 ◽  
Vol 30 (6) ◽  
pp. 451-456 ◽  
Author(s):  
C. Passera ◽  
M. Ferretti
Keyword(s):  
Bundle Sheath ◽  
Sulphate Uptake ◽  
Leaf Mesophyll ◽  
Bundle Sheath Cells ◽  
Maize Plants ◽  
Sheath Cells

scholarly journals Abscisic acid signalling determines susceptibility of bundle sheath cells to photoinhibition in high light-exposed Arabidopsis leaves

2014 ◽  
Vol 369 (1640) ◽  
pp. 20130234 ◽  
Author(s):  
Magdalena Gorecka ◽  
Ruben Alvarez-Fernandez ◽  
Katie Slattery ◽  
Lorna McAusland ◽  
Phillip A. Davey ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Bundle Sheath ◽  
High Light ◽  
Major Influence ◽  
Photochemical Quenching ◽  
Mesophyll Cells ◽  
Specific Expression ◽  
Rapid Induction ◽  
Vascular Parenchyma ◽  
Photosynthetic Responses

The rapid induction of the bundle sheath cell (BSC)-specific expression of ASCORBATE PEROXIDASE2 ( APX2 ) in high light (HL)-exposed leaves of Arabidopsis thaliana is, in part, regulated by the hormone abscisic acid (ABA) produced by vascular parenchyma cells. In this study, we provide more details of the ABA signalling that regulates APX2 expression and consider its importance in the photosynthetic responses of BSCs and whole leaves. This was done using a combination of analyses of gene expression and chlorophyll a fluorescence of both leaves and individual BSCs and mesophyll cells. The regulation of APX2 expression occurs by the combination of the protein kinase SnRK2.6 (OST1):protein phosphatase 2C ABI2 and a Gα (GPA1)-regulated signalling pathway. The use of an ost1-1/gpa1-4 mutant established that these signalling pathways are distinct but interact to regulate APX2 . In HL-exposed leaves, BSC chloroplasts were more susceptible to photoinhibition than those of mesophyll cells. The activity of the ABA-signalling network determined the degree of susceptibility of BSCs to photoinhibition by influencing non-photochemical quenching. By contrast, in HL-exposed whole leaves, ABA signalling did not have any major influence on their transcriptomes nor on their susceptibility to photoinhibition, except where guard cell responses were observed.

scholarly journals The rice bundle sheath produces reactive oxygen species during high light stress via NADPH oxidase

2020 ◽  
Author(s):  
Haiyan Xiong ◽  
Lei Hua ◽  
Ivan Reyna-Llorens ◽  
Yi Shi ◽  
Kun-Ming Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Light Stress ◽  
Photosynthetic Apparatus ◽  
Reactive Oxygen ◽  
Bundle Sheath ◽  
High Light ◽  
Ros Production ◽  
Oxygen Species ◽  
Mesophyll Cells

AbstractWhen exposed to high light plants produce reactive oxygen species (ROS). In Arabidopsis thaliana local accumulation of ROS preferentially takes place in bundle sheath strands, but little is known about how this response takes place. Using rice and the ROS probes diaminobenzidine and 2’,7’-dichlorodihydrofluorescein diacetate, we found that after exposure to high light, ROS were produced more rapidly in bundle sheath strands than mesophyll cells. This response was not affected either by CO2 supply or photorespiration. Consistent with these findings, deep sequencing of mRNA isolated from mesophyll or bundle sheath strands indicated balanced accumulation of transcripts encoding all major components of the photosynthetic apparatus. However, transcripts encoding several isoforms of the superoxide/H2O2-producing enzyme NADPH oxidase were more abundant in bundle sheath strands than mesophyll cells. ROS production in bundle sheath strands was reduced by blocking NADPH oxidase activity pharmacologically, but increased when the bundle sheath preferential RBOHA isoform of NADPH oxidase was over-expressed. NADPH oxidase mediated accumulation of ROS in the rice bundle sheath was detected in etiolated leaves lacking chlorophyll indicating that high light and NADPH oxidase-dependent ROS production is not dependent on photosynthesis.

scholarly journals High light induced accumulation of two isoforms of the CF1 alpha-subunit in mesophyll and bundle sheath chloroplasts of C4 plants.

2008 ◽  
Vol 55 (1) ◽  
pp. 175-182 ◽  
Author(s):  
Elzbieta Romanowska ◽  
Marta Powikrowska ◽  
Maksymilian Zienkiewicz ◽  
Anna Drozak ◽  
Berenika Pokorska
Keyword(s):  
Atp Synthase ◽  
Light Stress ◽  
Protective Role ◽  
Bundle Sheath ◽  
High Light ◽  
Alpha Subunit ◽  
Strong Increase ◽  
Panicum Maximum ◽  
Low Light ◽  
Light Irradiance

The effect of light irradiance on the amount of ATP synthase alpha-subunit in mesophyll (M) and bundle sheath (BS) chloroplasts of C(4) species such as maize (Zea mays L., type NADP-ME), millet (Panicum miliaceum, type NAD-ME) and guinea grass (Panicum maximum, type PEP-CK) was investigated in plants grown under high, moderate and low light intensities equal to 800, 350 and 50 micromol photons m(-2) s(-1), respectively. The results demonstrate that alpha-subunit of ATP synthase in both M and BS chloroplasts is altered by light intensity, but differently in the investigated species. Moreover, we identified two isoforms of the CF(1) alpha-subunit, called alpha and alpha. The CF(1) alpha-subunit was the major isoform and was present in all light conditions, whereas alpha was the minor isoform in low light. A strong increase in the level of the alpha-subunit in maize mesophyll and bundle sheath thylakoids was observed after 50 h of high light treatment. The alpha and alpha-subunits from investigated C(4) species displayed apparent molecular masses of 64 and 67 kDa, respectively, on SDS/PAGE. The presence of the alpha-subunit of ATPase was confirmed in isolated CF(1) complex, where it was recognized by antisera to the alpha-subunit. The N-terminal sequence of alpha-subunit is nearly identical to that of alpha. Our results indicate that both isoforms coexist in M and BS chloroplasts during plant growth at all irradiances. We suggest the existence in M and BS chloroplasts of C(4) plants of a mechanism(s) regulating the ATPase composition in response to light irradiance. Accumulation of the alpha isoform may have a protective role under high light stress against over protonation of the thylakoid lumen and photooxidative damage of PSII.

Photoinhibition and D1 protein degradation in mesophyll and agranal bundle sheath thylakoids of maize

2007 ◽  
Vol 34 (9) ◽  
pp. 844 ◽  
Author(s):  
Berenika Pokorska ◽  
Elzbieta Romanowska
Keyword(s):  
Degradation Rate ◽  
Zea Mays L ◽  
Proteolytic Enzymes ◽  
D1 Protein ◽  
Bundle Sheath ◽  
C3 Plants ◽  
Reaction Centre ◽  
C4 Plant ◽  
Donor Side ◽  
Maize Plants

Susceptibility of photosystem II complex (PSII) to photoinhibition and degradation of D1 protein has been described in the chloroplasts of C3 plants but so far, the PSII turnover has not been characterised in any C4 plant, which contains two types of chloroplasts differing biochemically and structurally. In maize (Zea mays L. Oleńka), chloroplasts located in mesophyll (M) develop grana, while bundle sheath (BS) chloroplasts are agranal. In this paper, we report the D1 protein phosphorylation, damage and proteolysis in mesophyll as well as in agranal bundle sheath thylakoids of maize plants. Photoinhibitory treatment (1800 μmol photons m–2 s–1) of isolated thylakoids led to donor side inhibition of PSII electron transport and then to damage of reaction centre in both M and BS thylakoids. Rate of D1 degradation rate was faster in BS than in M thylakoids, and the addition of ATP to incubation medium delayed D1 degradation in both types of thylakoids. Furthermore, we demonstrated that the proteases belonging to FtsH and Deg families were present but their amounts significantly differed in M and BS thylakoids. Protease inhibitor studies revealed that serine- and metallo-proteases were involved in degradation of D1 protein. Apparent existence of D1 degradation cycle and the presence of proteolytic enzymes responsible for this process in BS thylakoids confirm that PSII plays an important role in agranal membranes, and when damaged, D1 can be rapidly degraded to enable PSII repair and restoration in these membranes.

Sign in / Sign up

Export Citation Format

Share Document