Photoinhibition and D1 protein degradation in mesophyll and agranal bundle sheath thylakoids of maize

2007 ◽  
Vol 34 (9) ◽  
pp. 844 ◽  
Author(s):  
Berenika Pokorska ◽  
Elzbieta Romanowska
Keyword(s):  
Degradation Rate ◽  
Zea Mays L ◽  
Proteolytic Enzymes ◽  
D1 Protein ◽  
Bundle Sheath ◽  
C3 Plants ◽  
Reaction Centre ◽  
C4 Plant ◽  
Donor Side ◽  
Maize Plants

Susceptibility of photosystem II complex (PSII) to photoinhibition and degradation of D1 protein has been described in the chloroplasts of C3 plants but so far, the PSII turnover has not been characterised in any C4 plant, which contains two types of chloroplasts differing biochemically and structurally. In maize (Zea mays L. Oleńka), chloroplasts located in mesophyll (M) develop grana, while bundle sheath (BS) chloroplasts are agranal. In this paper, we report the D1 protein phosphorylation, damage and proteolysis in mesophyll as well as in agranal bundle sheath thylakoids of maize plants. Photoinhibitory treatment (1800 μmol photons m–2 s–1) of isolated thylakoids led to donor side inhibition of PSII electron transport and then to damage of reaction centre in both M and BS thylakoids. Rate of D1 degradation rate was faster in BS than in M thylakoids, and the addition of ATP to incubation medium delayed D1 degradation in both types of thylakoids. Furthermore, we demonstrated that the proteases belonging to FtsH and Deg families were present but their amounts significantly differed in M and BS thylakoids. Protease inhibitor studies revealed that serine- and metallo-proteases were involved in degradation of D1 protein. Apparent existence of D1 degradation cycle and the presence of proteolytic enzymes responsible for this process in BS thylakoids confirm that PSII plays an important role in agranal membranes, and when damaged, D1 can be rapidly degraded to enable PSII repair and restoration in these membranes.

scholarly journals Degradation of the D1 Protein of Photosystem-II Reaction Centre by Ultraviolet-B Radiation Requires the Presence of Functional Manganese on the Donor Side

2008 ◽  
Vol 227 (3) ◽  
pp. 723-729
Author(s):  
Roberto Barbato ◽  
Annalisa Frizzo ◽  
Giulia Friso ◽  
Fernanda Rigoni ◽  
Giorgio M. Giacometti
Keyword(s):  
Photosystem Ii ◽  
D1 Protein ◽  
Ultraviolet B ◽  
Reaction Centre ◽  
Donor Side ◽  
Ultraviolet B Radiation

scholarly journals Differential turnover of the photosystem II reaction centre D1 protein in mesophyll and bundle sheath chloroplasts of maize

2009 ◽  
Vol 1787 (10) ◽  
pp. 1161-1169 ◽  
Author(s):  
Berenika Pokorska ◽  
Maksymilian Zienkiewicz ◽  
Marta Powikrowska ◽  
Anna Drozak ◽  
Elzbieta Romanowska
Keyword(s):  
Photosystem Ii ◽  
D1 Protein ◽  
Bundle Sheath ◽  
Reaction Centre

Degradation of the D1 Protein of Photosystem-II Reaction Centre by Ultraviolet-B Radiation Requires the Presence of Functional Manganese on the Donor Side

1995 ◽  
Vol 227 (3) ◽  
pp. 723-729 ◽  
Author(s):  
Roberto Barbato ◽  
Annalisa Frizzo ◽  
Giulia Friso ◽  
Fernanda Rigoni ◽  
Giorgio M. Giacometti
Keyword(s):  
Photosystem Ii ◽  
D1 Protein ◽  
Ultraviolet B ◽  
Reaction Centre ◽  
Donor Side ◽  
Ultraviolet B Radiation

scholarly journals Activities of Antioxidant Enzymes in Mesophyll and Bundle Sheath Cell Chloroplasts of Maize Plants (Zea mays L.) Exposed to Salt Stress

2020 ◽  
Vol 6 (11) ◽  
pp. 47-56
Author(s):  
N. Aliyeva
Keyword(s):  
Zea Mays ◽  
Salt Stress ◽  
Antioxidant Defense ◽  
Zea Mays L ◽  
Bundle Sheath ◽  
Sheath Cell ◽  
Bundle Sheath Cell ◽  
Defense Systems ◽  
Antioxidant Defense Systems ◽  
Maize Plants

Antioxidant defense systems have been studied in the mesophyll (MC) and bundle sheath cell (BSC) chloroplasts of maize (Zea mays L.) leaves cultivated in an artificial climate chamber under various concentrations (0%, 1%, 2%, 3%) of NaCl. The amounts of some of the main products of lipid peroxidation malondialdehyde (MDA) and reactive oxygen species hydrogen peroxide (H2O2) as well as activities of superoxide dismutase (SOD) and ascorbate peroxidase (APO) were determined in MC and BSC chloroplasts. BSC chloroplasts were found to be more tolerant to salt stress compared with MC chloroplasts. The MDA amount increased in both chloroplasts. H2O2 was found to be localized mainly in MC chloroplasts at various NaCl concentrations. The SOD and APO activities increased in both chloroplasts of the plants exposed to salt stress.

scholarly journals Gluconacetobacter diazotrophicus Pal5 Enhances Plant Robustness Status under the Combination of Moderate Drought and Low Nitrogen Stress in Zea mays L.

2021 ◽  
Vol 9 (4) ◽  
pp. 870
Author(s):  
Muhammad Aammar Tufail ◽  
María Touceda-González ◽  
Ilaria Pertot ◽  
Ralf-Udo Ehlers
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Zea Mays L ◽  
Growth Promotion ◽  
Nitrogen Stress ◽  
Rrna Gene ◽  
N Fixation ◽  
Gluconacetobacter Diazotrophicus ◽  
Maize Plants ◽  
Low Nitrogen

Plant growth promoting endophytic bacteria, which can fix nitrogen, plays a vital role in plant growth promotion. Previous authors have evaluated the effect of Gluconacetobacter diazotrophicus Pal5 inoculation on plants subjected to different sources of abiotic stress on an individual basis. The present study aimed to appraise the effect of G. diazotrophicus inoculation on the amelioration of the individual and combined effects of drought and nitrogen stress in maize plants (Zea mays L.). A pot experiment was conducted whereby treatments consisted of maize plants cultivated under drought stress, in soil with a low nitrogen concentration and these two stress sources combined, with and without G. diazotrophicus seed inoculation. The inoculated plants showed increased plant biomass, chlorophyll content, plant nitrogen uptake, and water use efficiency. A general increase in copy numbers of G. diazotrophicus, based on 16S rRNA gene quantification, was detected under combined moderate stress, in addition to an increase in the abundance of genes involved in N fixation (nifH). Endophytic colonization of bacteria was negatively affected by severe stress treatments. Overall, G. diazotrophicus Pal5 can be considered as an effective tool to increase maize crop production under drought conditions with low application of nitrogen fertilizer.

scholarly journals Identification of DNA of pathogens from Fusarium genus in maize plants (Zea mays L.)

2020 ◽  
Author(s):  
Lidia Tumanova ◽  
◽  
Cristina Grajdieru ◽  
Valentin Mitin ◽  
◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Maize Plants

Photosynthesis in Phosphoenolpyruvate Carboxykinase-Type C4 Species: Properties of Nad-Malic Enzyme From Urochloa panicoides

1987 ◽  
Vol 14 (5) ◽  
pp. 517 ◽  
Author(s):  
JN Burnell
Keyword(s):  
Malic Enzyme ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Bundle Sheath ◽  
Photosynthetic Pathway ◽  
C4 Plant ◽  
C4 Species ◽  
Absolute Requirement ◽  
Pep Carboxykinase ◽  
Fructose 1,6 Bisphosphate ◽  
Regulatory Properties

NAD-malic enzyme (EC 1.1.1.39) was purified from bundle sheath strands of Urochloa panicoides (a phosphoenolpyruvate carboxykinase-type C4 plant) and its kinetic and regulatory properties were investigated. The native enzyme has a molecular weight of about 470 000 and is an octomer composed of two slightly different monomers which occur in a 1 : 1 ratio. The enzyme has an absolute requirement for Mn2+, is stimulated by CoA, acetyl CoA, fructose 1,6-bisphosphate and SO42- and is inhibited by HCO3, oxaloacetate, 2-oxoglutarate and pyruvate. The enzyme is shown to be localised in the mito- chondria. The purified NAD-malic enzyme is unable to catalyse the carboxylation of pyruvate according to the reverse reaction. These findings are discussed in relation to the C4 photosynthetic pathway and its possible role in PEP carboxykinase-type C4 plants.

scholarly journals Effects of the application of sewage sludge and petrochemical residue in maize culture as source of micro-nutrients on soils of Paraná state

2000 ◽  
Vol 43 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Ana T. Jordão Pigozzo ◽  
Marlene A. Gobbi ◽  
Ervim Lenzi ◽  
Eduardo B. Luchese
Keyword(s):  
Sewage Sludge ◽  
Zea Mays L ◽  
Absorption Spectrometry ◽  
Organic Residues ◽  
Constant Weight ◽  
Podzolic Soils ◽  
Aerial Parts ◽  
Used Motor Oil ◽  
Reference Plant ◽  
Maize Plants

An evaluation of the potential of two organic residues as source of micro-nutrients in three soils of Paraná state was studied. Treatments consisted of one dose (38 t.ha-1) of sewage sludge and residue of purified used motor oil both neutralized with CaO+MgO mixture in 3:1 rate. Maize (Zea mays L.) was used as reference plant. After 30 days of seeding, the aerial parts of the plants were cut. They were dried at 70ºC till constant weight and ground. Analysis was undertaken after nitric-perchloric digestion. Micro-nutrients levels in the soils and in the aerial parts of the plants were determined by atomic absorption spectrometry. The results showed a production of the aerial parts of the plants treated with sewage sludge higher than those in other treatments. The micro-nutrients in Terra Roxa - TR were higher than those of Latossolo Vervelho Escuro - LE (Deep Red Latisol) and Podzólico Vermelho - PV (Red Podzolic) soils, respectively. In the aerial part of the maize plants in treated soils concentrations of micro-nutrients Cu, Fe, Mn and Zn were higher than those without treatment.

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