Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines

PROTEOMICS ◽  
2014 ◽  
Vol 14 (9) ◽  
pp. 1071-1087 ◽  
Author(s):  
Baojian Guo ◽  
Yanhong Chen ◽  
Chuan Li ◽  
Tianya Wang ◽  
Rui Wang ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Nuclear Proteome ◽  
Parental Lines ◽  
Seedling Leaf

scholarly journals Combined Transcriptome and Proteome Analysis of Maize (Zea mays L.) Reveals A Complementary Profile in Response to Phosphate Deficiency

2021 ◽  
Vol 43 (2) ◽  
pp. 1142-1155
Author(s):  
Zhi Nie ◽  
Bowen Luo ◽  
Xiao Zhang ◽  
Ling Wu ◽  
Dan Liu ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Proteome Analysis ◽  
Transcript Level ◽  
Utilization Efficiency ◽  
Differentially Expressed ◽  
Phosphate Deficiency ◽  
Absolute Quantitation ◽  
Protein Levels ◽  
Isobaric Tags

A deficiency in the macronutrient phosphate (Pi) brings about various changes in plants at the morphological, physiological and molecular levels. However, the molecular mechanism for regulating Pi homeostasis in response to low-Pi remains poorly understood, particularly in maize (Zea mays L.), which is a staple crop and requires massive amounts of Pi. Therefore, in this study, we performed expression profiling of the shoots and roots of maize seedlings with Pi-tolerant genotype at both the transcriptomic and proteomic levels using RNA sequencing and isobaric tags for relative and absolute quantitation (iTRAQ). We identified 1944 differentially expressed transcripts and 340 differentially expressed proteins under low-Pi conditions. Most of the differentially expressed genes were clustered as regulators, such as transcription factors involved in the Pi signaling pathway at the transcript level. However, the more functional and metabolism-related genes showed expression changes at the protein level. Moreover, under low-Pi conditions, Pi transporters and phosphatases were specifically induced in the roots at both the transcript and protein levels, and increased amounts of mRNA and protein of two purple acid phosphatases (PAPs) and one UDP-sulfoquinovose synthase (SQD) were specifically detected in the roots. The new insights provided by this study will help to improve the P-utilization efficiency of maize.

scholarly journals Seed Vigour of Parental Lines and its Hybrid in Maize (Zea mays L.)

2021 ◽  
Vol 10 (01) ◽  
pp. 2230-2236
Author(s):  
Jayant Kumar ◽  
Mukesh Kumar ◽  
Arun Kumar ◽  
Manju Kumari
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Seed Vigour ◽  
Parental Lines

scholarly journals Inheritance of Yield Related Traits in a Half Diallel Crosses of Some Maize (Zea mays L.) Genotypes

2020 ◽  
Vol 18 (1) ◽  
pp. 18-25
Author(s):  
FMA Haydar
Keyword(s):  
Zea Mays ◽  
Grain Yield ◽  
Combining Ability ◽  
Zea Mays L ◽  
General Combining Ability ◽  
Gene Effect ◽  
Maize Populations ◽  
Diallel Crossing ◽  
Parental Lines ◽  
Half Diallel

Inheritance of grain yield, heterosis and combining ability were investigated in maize populations obtained from half-diallel crossing among six inbred parental lines. General (GCA) and specific (SCA) combining ability effects were significantly different among parental lines. The grain yield was under the partial gene effect. The parents P1, P3 and P5were considered suitable according to their yield contributing attributes couple with general combining ability effects. The midparentheterosis values ranged from -27.246 (P2 xP5) to 15.209% (P1 xP2) whereas the better parent heterosis values varied between 22.375(P2x P3) to 40.363% (P2 x P5) only seven crosses had higher grain yields. Of those crosses, P1×P2, P2×P5 and P1×P3 were considered most promising hybrids yielding heterosis as 15.880%, 20.363% and 40.363 respectively over their parents. The Agriculturists 2020; 18(1) 18-25

Investigation on direct and reciprocal crosses in maize ( Zea mays L.)

2008 ◽  
Vol 56 (2) ◽  
pp. 179-185
Author(s):  
Z. Bódi ◽  
P. Pepó ◽  
A. Kovács
Keyword(s):  
Zea Mays ◽  
Plant Height ◽  
Seed Production ◽  
Zea Mays L ◽  
Side Branch ◽  
Reciprocal Crosses ◽  
Positive Correlation ◽  
Stalk Diameter ◽  
Parental Lines ◽  
Medium Correlation

Seven traits of twelve hybrids derived from direct and reciprocal crosses of four parental lines were examined during three years in Hungary. No significant differences were found between the direct and reciprocal crosses for stalk diameter or leaf number over the average of three years in any of the hybrids. Significant differences in the number of primary tassel branches were observed between UDH5 and its reciprocal UDH8, and between UDH6 and its reciprocal UDH11. It was evident in both instances that the degree of heterosis in the reciprocal crosses far exceeded that in the direct ones. A high number of tassel branches was dominant over a low number, so this trait was preferentially inherited in the hybrids. A positive correlation was observed between plant height and main ear attachment height (r = 0.67**). A positive correlation (r = 0.89**) was also found between the length of the main tassel axis above the lowest and above the uppermost side-branch. A medium correlation was observed between the number of primary tassel branches and the plant height (r = −0.42**). The number of primary tassel branches exhibited the highest heterosis. These results can be utilized in practical selection and seed production.

scholarly journals Seedlings of zea mays l. (poaceae) and pterogyne nitens tul. (fabaceae): Morphoanatomic and terminological considerations / Plântulas de zea mays l. (poaceae) e pterogyne nitens tul. (fabaceae): Considerações morfoanatômicas e terminológicas

2021 ◽  
Vol 7 (12) ◽  
pp. 118717-118725
Author(s):  
Tatiane Martins Da Silva ◽  
Nara Alves Mendes Barella ◽  
Luiz Antonio De Souza
Keyword(s):  
Zea Mays ◽  
Undergraduate Students ◽  
Zea Mays L ◽  
Cell Layer ◽  
Primary Root ◽  
Transition Structure ◽  
Palisade Parenchyma ◽  
Wild Peanut ◽  
Seedling Leaf ◽  
Embryonic Root

Seedlings of Zea mays L. (maize), Poaceae, and Pterogyne nitens Tul. (wild peanut), Leguminosae, are described morphologically and anatomically in order to characterize the species, but particularly to disseminate the terminology about the seedling, which is little known by non-specialist researchers and undergraduate students. Seedlings were obtained in the laboratory, using Petri dishes. Seedling was considered as the initial plant development phase, which comprises the period from germination to formation of the eophyll. Zea mays seedling is hypogeal and cryptocotyledonous, and it consists of coleorhiza, considered the primary root, endogenous embryonic root, commonly considered in the literature as radicle, reduced hypocotyl, and coleoptile, considered here as eophyll. The second seedling leaf of Z. mays is made up of uniseriate epidermis and homogeneous mesophyll. Pterogyne nitens exhibits epigeal and phanerocotyledonous seedling, and consists of primary root, long hypocotyl, two cotyledons, epicotyl, and opposite eophylls difoliolated or trifoliolated. The hypocotyl has root/shoot transition structure and the eophylls are dorsiventral consisting of one cell layer palisade parenchyma and pluriseriate spongy parenchyma. Seedlings of both species show significant morphological and anatomical differences and specific terminology, especially that of Z. mays.

scholarly journals Mejoramiento de la línea MLS4-1 de maíz (Zea mays L.) a través del método de retrocruza.

2016 ◽  
Vol 7 (1) ◽  
pp. 62
Author(s):  
Jesús Arreola ◽  
Gustavo Burciaga ◽  
José Gutiérrez ◽  
Cristina Vega ◽  
Enrique Navarro
Keyword(s):  
Zea Mays ◽  
Combining Ability ◽  
Zea Mays L ◽  
Agronomic Traits ◽  
Research Work ◽  
Source Population ◽  
Male Line ◽  
Parental Lines ◽  
Original Line

This research work involves the improvement of the male line MLS4-1 of the hybrid AN-388 which was released by the Instituto Mexicano del Maíz of the Universidad Autónoma Agraria “Antonio Narro”. This line has excellent combining ability with other parental lines; however, it is susceptible to some diseases so that we are interested in improving it by using the backcross procedure. The Nepo population was used as a source population which is resistant to mildew spp. Three backcrosses and two recombinations were practiced before deriving lines and cross them with some testers. The objectives of this work were: 1. To evaluate the performance of recovered lines in combination with the original line MLS4-1, and with un-related testers, taking into account their combining ability and agronomic traits in relation to the hybrid AN-388, 2. To now the effectiveness of the backcross method to improve the MLS4-1 line. The results of this study allowed us to identify superior recovered lines that, in combination with the diverse testers, exceeded the agronomic value of thehybrid AN-388. Some recovered lines crossed with the MLS4-1 line produced excellent single crosses, indicating the possibility of getting new versions of the hybrid AN-388. Finally, we conclude that the improvement of the MLS4-1 line has permitted us to identify the best lines with higher combining ability and better agronomic traits.

scholarly journals Dissecting the Regulatory Network of Leaf Premature Senescence in Maize (Zea mays L.) Using Transcriptome Analysis of ZmELS5 Mutant

Genes ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 944
Author(s):  
Chai ◽  
Guo ◽  
Shi ◽  
Li ◽  
Tang ◽  
...  
Keyword(s):  
Zea Mays ◽  
Differentially Expressed Genes ◽  
Leaf Senescence ◽  
Transcriptome Analysis ◽  
Regulatory Network ◽  
Zea Mays L ◽  
Differentially Expressed ◽  
Premature Senescence ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Leaf premature senescence largely determines maize (Zea mays L.) grain yield and quality. A natural recessive premature-senescence mutant was selected from the breeding population, and near-isogenic lines were constructed using Jing24 as the recurrent parent. In the near-isogenic lines, the dominant homozygous material was wild-type (WT), and the recessive material of early leaf senescence was the premature-senescence-type ZmELS5. To identify major genes and regulatory mechanisms involved in leaf senescence, a transcriptome analysis of the ZmELS5 and WT near-isogenic lines (NILs) was performed. A total of 8,796 differentially expressed transcripts were identified between ZmELS5 and WT, including 3,811 up-regulated and 4,985 down-regulated transcripts. By combining gene ontology, Kyoto Encyclopedia of Genes and Genomes, gene set, and transcription factor enrichment analyses, key differentially expressed genes were screened. The senescence regulatory network was predicted based on these key differentially expressed genes, which indicated that the senescence process is mainly regulated by bHLH, WRKY, and AP2/EREBP family transcription factors, leading to the accumulations of jasmonic acid and ethylene. This causes stress responses and reductions in the chlorophyll a/b-binding protein activity level. Then, decreased ATP synthase activity leads to increased photosystem II photodamage, ultimately leading to leaf senescence.

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