Molecular level differentiation between end-capped and intramolecular azofunctional oligo(ε-caprolactone) positional isomers through liquid chromatography multistage mass spectrometry

Investigation of archeological and paleontological artifacts at the molecular level has become a trend over the recent years. Paleogenomics provides information about the evolution of ancient species; paleoproteomics was recently established as branch of conventional proteomics dedicated to the study of proteomes of extinct organisms. At the same time paleolipidomics, which could focus on the investigation of lipids of ancient species, is not developed yet, though the investigation of fossil lipids may have potential to provide information about past diseases or diet. Here we present the investigation of an ancient fossil bone of a Siberian mammoth using combined proteomics and lipidomics approaches based on high-resolution tandem mass spectrometry coupled with liquid chromatography (LC-MS/MS). Using various experimental approaches, we have reliably identified 98 proteins and 73 lipids in three tissue regions of the mammoth bone. We have also compared the proteome profile of ancient mammoth bone with those of a modern elephant.

Download Full-text

Simultaneous Quantification of Mixed-Acid Triacylglycerol Positional Isomers and Enantiomers in Palm Oil and Lard by Chiral High-Performance Liquid Chromatography Coupled with Mass Spectrometry

Symmetry ◽

10.3390/sym12091385 ◽

2020 ◽

Vol 12 (9) ◽

pp. 1385

Author(s):

Toshiharu Nagai ◽

Tetsuaki Kinoshita ◽

Erika Kasamatsu ◽

Kazuaki Yoshinaga ◽

Hoyo Mizobe ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Palm Oil ◽

High Performance ◽

Positional Distribution ◽

Positional Isomers ◽

Mixed Acid ◽

Edible Fats ◽

Natural Oils ◽

The Difference

Palm oil and lard are edible fats which are rich in palmitic (P) and oleic acids (O). In this study, triacylglycerol (TAG) positional isomers (symmetric and asymmetric isomers) and enantiomers (asymmetric isomers) in palm oil and lard were quantified simultaneously by using liquid chromatography/mass spectrometry. The CHIRALPAK IF-3 column used in our previous study recognized the difference of TAG isomers consisting of P and O in palm oil and lard, separated sn-OPP/sn-PPO/sn-POP and sn-OPO/sn-OOP/sn-POO into each isomer peak, and enabled the quantification of these TAG isomers with good recovery (95–120%). Although sn-POP and sn-OPO were the major TAGs in palm oil and lard, a comparison of the abundance ratios of TAG enantiomers such as sn-PPO/sn-OPP and sn-OOP/sn-POO revealed that there were slightly more TAG enantiomers with O at the sn-1 position and P at the sn-3 position in palm oil and P at the sn-1 position and O at the sn-3 position in lard. These results were consistent with previous reports for the positional distribution of fatty acids of palm oil and lard. This is the first study that has enabled all TAG isomers consisting of P and O in natural oils and fats to be individually quantified by mass spectrometry.

Download Full-text